Comparison of chemical washing and physical cell-disruption approaches to assess the surface adsorption and internalization of cadmium by Cupriavidus metallidurans CH34

•Subcellular distribution of cadmium in Cupriavidus metallidurans CH34 cells.•Comparison of a chemical (EDTA washing) and a physical method (physical disruption).•EDTA washings strongly overestimated membrane-bound Cd concentrations.•The physical method revealed surprisingly over 80% of Cd internali...

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Veröffentlicht in:	Journal of hazardous materials 2014-05, Vol.273, p.231-238
Hauptverfasser:	Desaunay, Aurélien, Martins, Jean M.F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Applied sciences Bacteria Biological and medical sciences Biological Transport Biotechnology Cadmium - chemistry Cadmium - metabolism Cd biosorption Cell Membrane - chemistry Cell Membrane - metabolism Cell Membrane - ultrastructure Chelating Agents - chemistry Chemical engineering Cupriavidus - chemistry Cupriavidus - metabolism Cupriavidus - ultrastructure Cytoplasm - metabolism Decontamination. Miscellaneous Edetic Acid - chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology Internalization Methods. Procedures. Technologies Microscopy, Electron, Transmission Others Pollution Pressure Soil and sediments pollution Subcellular distribution Surface complexation Various methods and equipments
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