Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents
Three strains of regular, long, Gram-stain-positive bacterial rods were isolated using TPY, M.R.S. and Rogosa agar under anaerobic conditions from the digestive tract of wild mice (Mus musculus). All 16S rRNA gene sequences of these isolates were most similar to sequences of Lactobacillus gasseri AT...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2014-05, Vol.64 (Pt 5), p.1526-1533 |
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| container_issue | Pt 5 |
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| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 64 |
| creator | Killer, J Havlík, J Vlková, E Rada, V Pechar, R Benada, O Kopečný, J Kofroňová, O Sechovcová, H |
| description | Three strains of regular, long, Gram-stain-positive bacterial rods were isolated using TPY, M.R.S. and Rogosa agar under anaerobic conditions from the digestive tract of wild mice (Mus musculus). All 16S rRNA gene sequences of these isolates were most similar to sequences of Lactobacillus gasseri ATCC 33323T and Lactobacillus johnsonii ATCC 33200T (97.3% and 97.2% sequence similarities, respectively). The novel strains shared 99.2-99.6% 16S rRNA gene sequence similarities. Type strains of L. gasseri and L. johnsonii were also most related to the newly isolated strains according to rpoA (83.9-84.0% similarities), pheS (84.6-87.8%), atpA (86.2-87.7%), hsp60 (89.4-90.4%) and tuf (92.7-93.6%) gene sequence similarities. Phylogenetic studies based on 16S rRNA, hsp60, rpoA, atpA and pheS gene sequences, other genotypic and many phenotypic characteristics (results of API 50 CHL, Rapid ID 32A and API ZYM biochemical tests; cellular fatty acid profiles; cellular polar lipid profiles; end products of glucose fermentation) showed that these bacterial strains represent a novel species within the genus Lactobacillus. The name Lactobacillus rodentium sp. nov. is proposed to accommodate this group of new isolates. The type strain is MYMRS/TLU1T (=DSM 24759T=CCM 7945T). |
| doi_str_mv | 10.1099/ijs.0.054924-0 |
| format | Article |
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All 16S rRNA gene sequences of these isolates were most similar to sequences of Lactobacillus gasseri ATCC 33323T and Lactobacillus johnsonii ATCC 33200T (97.3% and 97.2% sequence similarities, respectively). The novel strains shared 99.2-99.6% 16S rRNA gene sequence similarities. Type strains of L. gasseri and L. johnsonii were also most related to the newly isolated strains according to rpoA (83.9-84.0% similarities), pheS (84.6-87.8%), atpA (86.2-87.7%), hsp60 (89.4-90.4%) and tuf (92.7-93.6%) gene sequence similarities. Phylogenetic studies based on 16S rRNA, hsp60, rpoA, atpA and pheS gene sequences, other genotypic and many phenotypic characteristics (results of API 50 CHL, Rapid ID 32A and API ZYM biochemical tests; cellular fatty acid profiles; cellular polar lipid profiles; end products of glucose fermentation) showed that these bacterial strains represent a novel species within the genus Lactobacillus. The name Lactobacillus rodentium sp. nov. is proposed to accommodate this group of new isolates. The type strain is MYMRS/TLU1T (=DSM 24759T=CCM 7945T).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.054924-0</identifier><identifier>PMID: 24478214</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Bacterial Typing Techniques ; Base Composition ; Czech Republic ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Fermentation ; Genes, Bacterial ; Intestine, Small - microbiology ; Lactobacillus - classification ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Mice - microbiology ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Peptidoglycan - chemistry ; Phylogeny ; Rectum - microbiology ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA</subject><ispartof>International journal of systematic and evolutionary microbiology, 2014-05, Vol.64 (Pt 5), p.1526-1533</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-c3a8aa1e3f8fd65d3ad87bc87f8e070407e40ac49412138a431503dd9549e2e93</citedby><cites>FETCH-LOGICAL-c401t-c3a8aa1e3f8fd65d3ad87bc87f8e070407e40ac49412138a431503dd9549e2e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24478214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Killer, J</creatorcontrib><creatorcontrib>Havlík, J</creatorcontrib><creatorcontrib>Vlková, E</creatorcontrib><creatorcontrib>Rada, V</creatorcontrib><creatorcontrib>Pechar, R</creatorcontrib><creatorcontrib>Benada, O</creatorcontrib><creatorcontrib>Kopečný, J</creatorcontrib><creatorcontrib>Kofroňová, O</creatorcontrib><creatorcontrib>Sechovcová, H</creatorcontrib><title>Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Three strains of regular, long, Gram-stain-positive bacterial rods were isolated using TPY, M.R.S. and Rogosa agar under anaerobic conditions from the digestive tract of wild mice (Mus musculus). All 16S rRNA gene sequences of these isolates were most similar to sequences of Lactobacillus gasseri ATCC 33323T and Lactobacillus johnsonii ATCC 33200T (97.3% and 97.2% sequence similarities, respectively). The novel strains shared 99.2-99.6% 16S rRNA gene sequence similarities. Type strains of L. gasseri and L. johnsonii were also most related to the newly isolated strains according to rpoA (83.9-84.0% similarities), pheS (84.6-87.8%), atpA (86.2-87.7%), hsp60 (89.4-90.4%) and tuf (92.7-93.6%) gene sequence similarities. Phylogenetic studies based on 16S rRNA, hsp60, rpoA, atpA and pheS gene sequences, other genotypic and many phenotypic characteristics (results of API 50 CHL, Rapid ID 32A and API ZYM biochemical tests; cellular fatty acid profiles; cellular polar lipid profiles; end products of glucose fermentation) showed that these bacterial strains represent a novel species within the genus Lactobacillus. The name Lactobacillus rodentium sp. nov. is proposed to accommodate this group of new isolates. The type strain is MYMRS/TLU1T (=DSM 24759T=CCM 7945T).</description><subject>Animals</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Czech Republic</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Fermentation</subject><subject>Genes, Bacterial</subject><subject>Intestine, Small - microbiology</subject><subject>Lactobacillus - classification</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - isolation & purification</subject><subject>Mice - microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Peptidoglycan - chemistry</subject><subject>Phylogeny</subject><subject>Rectum - microbiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhC0EoqVw5Yh85EDC-pHEOSLEU5W4wNly_QBXSV3spIh_j6uWnna1mhntfAhdEigJtO2tX6YSSqh4S3kBR2hKeF0XFTB-fNhpPUFnKS0B8gHgFE0o542ghE_R61zpISyU9l03JhyDsavBjz1O6xKvwqa8wS6GHg9fFhv_adPgNxYPMbtwcPjHd2ZvSufoxKku2Yv9nKGPx4f3--di_vb0cn83LzQHMhSaKaEUscwJZ-rKMGVEs9CiccJCAxway0Fp3nJCCROKM5LrGNPmjpbals3Q9S53HcP3mD-SvU_adp1a2TAmSSrKcgrLrhkqd1IdQ0rROrmOvlfxVxKQW34y85Mgd_zk1nC1zx4XvTUH-T8w9gdYX2sD</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Killer, J</creator><creator>Havlík, J</creator><creator>Vlková, E</creator><creator>Rada, V</creator><creator>Pechar, R</creator><creator>Benada, O</creator><creator>Kopečný, J</creator><creator>Kofroňová, O</creator><creator>Sechovcová, H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140501</creationdate><title>Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents</title><author>Killer, J ; 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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Typing Techniques Base Composition Czech Republic DNA, Bacterial - genetics Fatty Acids - chemistry Fermentation Genes, Bacterial Intestine, Small - microbiology Lactobacillus - classification Lactobacillus - genetics Lactobacillus - isolation & purification Mice - microbiology Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan - chemistry Phylogeny Rectum - microbiology RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| title | Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents |
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