N Latex FLC serum free light-chain assays in patients with renal impairment
The aim of this study was to establish ranges for N Latex free light-chain (FLC) monoclonal-based nephelometric assays in patients with renal impairment. In this retrospective study, serum samples from 284 patients with chronic kidney disease (CKD) stages 1–5 were measured with N Latex and Freelite™...
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| Veröffentlicht in: | Clinical chemistry and laboratory medicine 2014-06, Vol.52 (6), p.853-859 |
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| creator | Jacobs, Joannes F.M. Hoedemakers, Rein M.J. Teunissen, Elisa te Velthuis, Henk |
| description | The aim of this study was to establish ranges for N Latex free light-chain (FLC) monoclonal-based nephelometric assays in patients with renal impairment.
In this retrospective study, serum samples from 284 patients with chronic kidney disease (CKD) stages 1–5 were measured with N Latex and Freelite™ FLC reagents on the Siemens BN™II system and compared with controls without renal impairment.
Both κFLC and λFLC concentrations increased with the N Latex FLC and the Freelite™ assays with each increment in CKD stage. No difference was found in FLC κ concentrations between the two methods. In patients with renal failure, N Latex FLC detected higher concentrations of λFLC (CKD5 median, 128 mg/L; 95% range, 43–302) compared with Freelite™ (89.5 mg/L, 35–197) (p |
| doi_str_mv | 10.1515/cclm-2013-0864 |
| format | Article |
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In this retrospective study, serum samples from 284 patients with chronic kidney disease (CKD) stages 1–5 were measured with N Latex and Freelite™ FLC reagents on the Siemens BN™II system and compared with controls without renal impairment.
Both κFLC and λFLC concentrations increased with the N Latex FLC and the Freelite™ assays with each increment in CKD stage. No difference was found in FLC κ concentrations between the two methods. In patients with renal failure, N Latex FLC detected higher concentrations of λFLC (CKD5 median, 128 mg/L; 95% range, 43–302) compared with Freelite™ (89.5 mg/L, 35–197) (p<0.0001). This resulted in significantly different κ/λ ratios in patients with CKD for the two tests. The Freelite™ κ/λ ratio in the CKD5 group (median, 1.22; min–max, 0.22–2.70) was significantly increased compared with healthy controls (p<0.0001), and several individual samples were outside the reference range for healthy controls (0.26–1.65). In contrast, none of the 284 patients with CKD had an FLC κ/λ ratio exceeding the N Latex reference limits for healthy controls (0.31–1.56). The N Latex FLC κ/λ ratio in the CKD5 group (0.69, 0.32–1.54) was significantly lower compared with the control group (p<0.0001).
These findings demonstrate that the N Latex FLC κ/λ ratio in patients with renal failure did not differ from the reference limits for healthy controls.</description><identifier>ISSN: 1434-6621</identifier><identifier>EISSN: 1437-4331</identifier><identifier>DOI: 10.1515/cclm-2013-0864</identifier><identifier>PMID: 24406282</identifier><language>eng</language><publisher>Germany: De Gruyter</publisher><subject>Blood Chemical Analysis - methods ; chronic kidney disease (CKD) ; free light-chain (FLC) ; free light-chains (FLCs) ; Humans ; Immunoglobulin Light Chains - blood ; Latex ; N Latex FLC ; renal failure ; Renal Insufficiency, Chronic - blood ; Retrospective Studies</subject><ispartof>Clinical chemistry and laboratory medicine, 2014-06, Vol.52 (6), p.853-859</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-2296b2d399e4108b1e08c171c1888942087cfc5ff7444bc62929f14f9283b2aa3</citedby><cites>FETCH-LOGICAL-c348t-2296b2d399e4108b1e08c171c1888942087cfc5ff7444bc62929f14f9283b2aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.degruyter.com/document/doi/10.1515/cclm-2013-0864/pdf$$EPDF$$P50$$Gwalterdegruyter$$H</linktopdf><linktohtml>$$Uhttps://www.degruyter.com/document/doi/10.1515/cclm-2013-0864/html$$EHTML$$P50$$Gwalterdegruyter$$H</linktohtml><link.rule.ids>315,782,786,27933,27934,66763,68547</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24406282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jacobs, Joannes F.M.</creatorcontrib><creatorcontrib>Hoedemakers, Rein M.J.</creatorcontrib><creatorcontrib>Teunissen, Elisa</creatorcontrib><creatorcontrib>te Velthuis, Henk</creatorcontrib><title>N Latex FLC serum free light-chain assays in patients with renal impairment</title><title>Clinical chemistry and laboratory medicine</title><addtitle>Clin Chem Lab Med</addtitle><description>The aim of this study was to establish ranges for N Latex free light-chain (FLC) monoclonal-based nephelometric assays in patients with renal impairment.
In this retrospective study, serum samples from 284 patients with chronic kidney disease (CKD) stages 1–5 were measured with N Latex and Freelite™ FLC reagents on the Siemens BN™II system and compared with controls without renal impairment.
Both κFLC and λFLC concentrations increased with the N Latex FLC and the Freelite™ assays with each increment in CKD stage. No difference was found in FLC κ concentrations between the two methods. In patients with renal failure, N Latex FLC detected higher concentrations of λFLC (CKD5 median, 128 mg/L; 95% range, 43–302) compared with Freelite™ (89.5 mg/L, 35–197) (p<0.0001). This resulted in significantly different κ/λ ratios in patients with CKD for the two tests. The Freelite™ κ/λ ratio in the CKD5 group (median, 1.22; min–max, 0.22–2.70) was significantly increased compared with healthy controls (p<0.0001), and several individual samples were outside the reference range for healthy controls (0.26–1.65). In contrast, none of the 284 patients with CKD had an FLC κ/λ ratio exceeding the N Latex reference limits for healthy controls (0.31–1.56). The N Latex FLC κ/λ ratio in the CKD5 group (0.69, 0.32–1.54) was significantly lower compared with the control group (p<0.0001).
These findings demonstrate that the N Latex FLC κ/λ ratio in patients with renal failure did not differ from the reference limits for healthy controls.</description><subject>Blood Chemical Analysis - methods</subject><subject>chronic kidney disease (CKD)</subject><subject>free light-chain (FLC)</subject><subject>free light-chains (FLCs)</subject><subject>Humans</subject><subject>Immunoglobulin Light Chains - blood</subject><subject>Latex</subject><subject>N Latex FLC</subject><subject>renal failure</subject><subject>Renal Insufficiency, Chronic - blood</subject><subject>Retrospective Studies</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EolBYGZFHlhSf7TiOxIIqCogIFpgtx3XaVPnCdlT670loYWO6V6fnXp0ehK6AzCCG-NaYqo4oARYRKfgROgPOkogzBsc_mUdCUJigc-83hEAc8-QUTSjnRFBJz9DLK850sF94kc2xt66vceGsxVW5WofIrHXZYO293nk8pE6H0jbB420Z1tjZRle4rDtdunpYX6CTQlfeXh7mFH0sHt7nT1H29vg8v88iw7gMEaWpyOmSpanlQGQOlkgDCRiQUqacEpmYwsRFkXDOcyNoStMCeJFSyXKqNZuim31v59rP3vqg6tIbW1W6sW3vFcSUCgmCkAGd7VHjWu-dLVTnylq7nQKiRoFqFKhGgWoUOBxcH7r7vLbLP_zX2ADc7YGtroJ1S7ty_W4IatP2bvDh_2mOh5dixr4Bun1-MQ</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Jacobs, Joannes F.M.</creator><creator>Hoedemakers, Rein M.J.</creator><creator>Teunissen, Elisa</creator><creator>te Velthuis, Henk</creator><general>De Gruyter</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>N Latex FLC serum free light-chain assays in patients with renal impairment</title><author>Jacobs, Joannes F.M. ; Hoedemakers, Rein M.J. ; Teunissen, Elisa ; te Velthuis, Henk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-2296b2d399e4108b1e08c171c1888942087cfc5ff7444bc62929f14f9283b2aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Blood Chemical Analysis - methods</topic><topic>chronic kidney disease (CKD)</topic><topic>free light-chain (FLC)</topic><topic>free light-chains (FLCs)</topic><topic>Humans</topic><topic>Immunoglobulin Light Chains - blood</topic><topic>Latex</topic><topic>N Latex FLC</topic><topic>renal failure</topic><topic>Renal Insufficiency, Chronic - blood</topic><topic>Retrospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jacobs, Joannes F.M.</creatorcontrib><creatorcontrib>Hoedemakers, Rein M.J.</creatorcontrib><creatorcontrib>Teunissen, Elisa</creatorcontrib><creatorcontrib>te Velthuis, Henk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry and laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jacobs, Joannes F.M.</au><au>Hoedemakers, Rein M.J.</au><au>Teunissen, Elisa</au><au>te Velthuis, Henk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N Latex FLC serum free light-chain assays in patients with renal impairment</atitle><jtitle>Clinical chemistry and laboratory medicine</jtitle><addtitle>Clin Chem Lab Med</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>52</volume><issue>6</issue><spage>853</spage><epage>859</epage><pages>853-859</pages><issn>1434-6621</issn><eissn>1437-4331</eissn><abstract>The aim of this study was to establish ranges for N Latex free light-chain (FLC) monoclonal-based nephelometric assays in patients with renal impairment.
In this retrospective study, serum samples from 284 patients with chronic kidney disease (CKD) stages 1–5 were measured with N Latex and Freelite™ FLC reagents on the Siemens BN™II system and compared with controls without renal impairment.
Both κFLC and λFLC concentrations increased with the N Latex FLC and the Freelite™ assays with each increment in CKD stage. No difference was found in FLC κ concentrations between the two methods. In patients with renal failure, N Latex FLC detected higher concentrations of λFLC (CKD5 median, 128 mg/L; 95% range, 43–302) compared with Freelite™ (89.5 mg/L, 35–197) (p<0.0001). This resulted in significantly different κ/λ ratios in patients with CKD for the two tests. The Freelite™ κ/λ ratio in the CKD5 group (median, 1.22; min–max, 0.22–2.70) was significantly increased compared with healthy controls (p<0.0001), and several individual samples were outside the reference range for healthy controls (0.26–1.65). In contrast, none of the 284 patients with CKD had an FLC κ/λ ratio exceeding the N Latex reference limits for healthy controls (0.31–1.56). The N Latex FLC κ/λ ratio in the CKD5 group (0.69, 0.32–1.54) was significantly lower compared with the control group (p<0.0001).
These findings demonstrate that the N Latex FLC κ/λ ratio in patients with renal failure did not differ from the reference limits for healthy controls.</abstract><cop>Germany</cop><pub>De Gruyter</pub><pmid>24406282</pmid><doi>10.1515/cclm-2013-0864</doi><tpages>7</tpages></addata></record> |
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| ispartof | Clinical chemistry and laboratory medicine, 2014-06, Vol.52 (6), p.853-859 |
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| source | MEDLINE; De Gruyter journals |
| subjects | Blood Chemical Analysis - methods chronic kidney disease (CKD) free light-chain (FLC) free light-chains (FLCs) Humans Immunoglobulin Light Chains - blood Latex N Latex FLC renal failure Renal Insufficiency, Chronic - blood Retrospective Studies |
| title | N Latex FLC serum free light-chain assays in patients with renal impairment |
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