Increasing gene expression in yeast by fusion to ubiquitin

Heterologous gene expression in yeast can be increased up to several hundred-fold by expressing a foreign gene as a fusion to the ubiquitin gene. An endogenous yeast endoprotease (Ub-Xase) removes the ubiquitin from the fusion product to produce the authentic protein. The utility of this technique h...

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Veröffentlicht in:	The Journal of biological chemistry 1989-05, Vol.264 (13), p.7715-7719
Hauptverfasser:	Ecker, D J, Stadel, J M, Butt, T R, Marsh, J A, Monia, B P, Powers, D A, Gorman, J A, Clark, P E, Warren, F, Shatzman, A, Crooker, S T
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Differentiation, T-Lymphocyte - genetics Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology GENE Gene expression Gene Expression Regulation gene fusion GENES Genetic engineering Genetic technics Genetic Vectors GTP-Binding Proteins - genetics guanine nucleotide-binding protein Methods. Procedures. Technologies Molecular and cellular biology Molecular cloning Molecular genetics plasminogen activator Protein Processing, Post-Translational PROTEIN SYNTHESIS PROTEINAS PROTEINE PROTEINS Recombinant Fusion Proteins - genetics Recombinant Proteins - genetics SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics sCD4 protein SINTESIS DE PROTEINAS Solubility Structure-Activity Relationship SYNTHESE PROTEIQUE Ubiquitins - genetics Urokinase-Type Plasminogen Activator - genetics
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