effect of vaccination, ploidy and smolt production regime on pathological melanin depositions in muscle tissue of Atlantic salmon, Salmo salar L
The presence of melanin in muscle fillets of farmed salmon represents a considerable quality problem for the salmon industry with major economic concerns. In this study, we have examined the presence of abnormal pigmentation in vaccinated versus unvaccinated Atlantic salmon, Salmo salar L., and eval...
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| Veröffentlicht in: | Journal of fish diseases 2014-04, Vol.37 (4), p.327-340 |
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| creator | Larsen, H A S Austbø, L Nødtvedt, A Fraser, T W K Rimstad, E Fjelldal, P G Hansen, T Koppang, E O |
| description | The presence of melanin in muscle fillets of farmed salmon represents a considerable quality problem for the salmon industry with major economic concerns. In this study, we have examined the presence of abnormal pigmentation in vaccinated versus unvaccinated Atlantic salmon, Salmo salar L., and evaluated possible differences between diploid and triploid fish. Furthermore, the impact of the smolt production regime at ambient (4.5 °C) versus elevated temperature (16 °C) was investigated. Pigmented muscle spots were analysed for the expression of genes involved in melanization (tyrosinase gene family) and immune‐related response in addition to morphological investigations. The proportion of fish with intramuscular melanin deposits was not significantly different between vaccinated and unvaccinated fish, regardless of ploidy. However, an interaction between vaccination and smolt regime was shown, where smoltification at elevated temperature after vaccination increased the number of affected individuals compared with vaccination followed by simulated natural smoltification. Furthermore, there were overall more fish with melanin spots amongst the triploids compared with their diploid counterparts. Transcription of the tyrosinase gene family confirmed an onsite melanogenesis in all pigment spots. The histological examination and the expression of the immune‐related genes revealed a chronic polyphasic myopathy that was not affected by vaccination, ploidy or smolt production regime. |
| doi_str_mv | 10.1111/jfd.12106 |
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In this study, we have examined the presence of abnormal pigmentation in vaccinated versus unvaccinated Atlantic salmon, Salmo salar L., and evaluated possible differences between diploid and triploid fish. Furthermore, the impact of the smolt production regime at ambient (4.5 °C) versus elevated temperature (16 °C) was investigated. Pigmented muscle spots were analysed for the expression of genes involved in melanization (tyrosinase gene family) and immune‐related response in addition to morphological investigations. The proportion of fish with intramuscular melanin deposits was not significantly different between vaccinated and unvaccinated fish, regardless of ploidy. However, an interaction between vaccination and smolt regime was shown, where smoltification at elevated temperature after vaccination increased the number of affected individuals compared with vaccination followed by simulated natural smoltification. Furthermore, there were overall more fish with melanin spots amongst the triploids compared with their diploid counterparts. Transcription of the tyrosinase gene family confirmed an onsite melanogenesis in all pigment spots. The histological examination and the expression of the immune‐related genes revealed a chronic polyphasic myopathy that was not affected by vaccination, ploidy or smolt production regime.</description><identifier>ISSN: 0140-7775</identifier><identifier>EISSN: 1365-2761</identifier><identifier>DOI: 10.1111/jfd.12106</identifier><identifier>PMID: 23646928</identifier><language>eng</language><publisher>England: Blackwell Science</publisher><subject>Animals ; Aquaculture ; diploid ; Diploidy ; farmed fish ; fillets ; Fish Diseases - genetics ; Fish Diseases - pathology ; fish industry ; Fish Proteins - genetics ; Fish Proteins - metabolism ; genes ; Inflammation - etiology ; Inflammation - genetics ; Inflammation - pathology ; Inflammation - veterinary ; Marine ; melanin ; Melanins - metabolism ; melanization ; melanogenesis ; melanomacrophage ; muscle tissues ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - pathology ; muscular diseases ; Ploidies ; Real-Time Polymerase Chain Reaction - veterinary ; Salmo salar ; smoltification ; Temperature ; triploid ; Triploidy ; vaccination ; Vaccination - adverse effects</subject><ispartof>Journal of fish diseases, 2014-04, Vol.37 (4), p.327-340</ispartof><rights>2013 John Wiley & Sons Ltd</rights><rights>2013 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfd.12106$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfd.12106$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23646928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Larsen, H A S</creatorcontrib><creatorcontrib>Austbø, L</creatorcontrib><creatorcontrib>Nødtvedt, A</creatorcontrib><creatorcontrib>Fraser, T W K</creatorcontrib><creatorcontrib>Rimstad, E</creatorcontrib><creatorcontrib>Fjelldal, P G</creatorcontrib><creatorcontrib>Hansen, T</creatorcontrib><creatorcontrib>Koppang, E O</creatorcontrib><title>effect of vaccination, ploidy and smolt production regime on pathological melanin depositions in muscle tissue of Atlantic salmon, Salmo salar L</title><title>Journal of fish diseases</title><addtitle>J Fish Dis</addtitle><description>The presence of melanin in muscle fillets of farmed salmon represents a considerable quality problem for the salmon industry with major economic concerns. In this study, we have examined the presence of abnormal pigmentation in vaccinated versus unvaccinated Atlantic salmon, Salmo salar L., and evaluated possible differences between diploid and triploid fish. Furthermore, the impact of the smolt production regime at ambient (4.5 °C) versus elevated temperature (16 °C) was investigated. Pigmented muscle spots were analysed for the expression of genes involved in melanization (tyrosinase gene family) and immune‐related response in addition to morphological investigations. The proportion of fish with intramuscular melanin deposits was not significantly different between vaccinated and unvaccinated fish, regardless of ploidy. However, an interaction between vaccination and smolt regime was shown, where smoltification at elevated temperature after vaccination increased the number of affected individuals compared with vaccination followed by simulated natural smoltification. Furthermore, there were overall more fish with melanin spots amongst the triploids compared with their diploid counterparts. Transcription of the tyrosinase gene family confirmed an onsite melanogenesis in all pigment spots. The histological examination and the expression of the immune‐related genes revealed a chronic polyphasic myopathy that was not affected by vaccination, ploidy or smolt production regime.</description><subject>Animals</subject><subject>Aquaculture</subject><subject>diploid</subject><subject>Diploidy</subject><subject>farmed fish</subject><subject>fillets</subject><subject>Fish Diseases - genetics</subject><subject>Fish Diseases - pathology</subject><subject>fish industry</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>genes</subject><subject>Inflammation - etiology</subject><subject>Inflammation - genetics</subject><subject>Inflammation - pathology</subject><subject>Inflammation - veterinary</subject><subject>Marine</subject><subject>melanin</subject><subject>Melanins - metabolism</subject><subject>melanization</subject><subject>melanogenesis</subject><subject>melanomacrophage</subject><subject>muscle tissues</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - pathology</subject><subject>muscular diseases</subject><subject>Ploidies</subject><subject>Real-Time Polymerase Chain Reaction - veterinary</subject><subject>Salmo salar</subject><subject>smoltification</subject><subject>Temperature</subject><subject>triploid</subject><subject>Triploidy</subject><subject>vaccination</subject><subject>Vaccination - adverse effects</subject><issn>0140-7775</issn><issn>1365-2761</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks9u1DAQxi0EokvhwAuAjxxI6_G_bI5toQW0AomlqsTFcu3x4uLES5wA-xY8Mkm39IwvM6P5fR7bnwl5DuwIpnV8E_wRcGD6AVmA0KritYaHZMFAsqqua3VAnpRywxjUCvRjcsCFlrrhywX5gyGgG2gO9Kd1LnZ2iLl7TbcpR7-jtvO0tDkNdNtnP7q5SXvcxBbplG3t8C2nvInOJtpisl3sqMdtLnEmC53KdiwuIR1iKSPOc06GiRuio8Wmdp61nuNc2Z6unpJHwaaCz-7iIbk8f_vl7F21-nTx_uxkVQUhta6CR-mtXEpmxdL5BoMHhigQIFjNJOpGiIZxZMp5zlgj0SEoWMK14I0K4pC82u87XezHiGUwbSwO03Q2zGMxoDg0wGsu_wMFXUslmhl9cYeO1y16s-1ja_ud-ffgE3C8B37FhLv7PjAzO2kmJ82tk-bD-ZvbZFJUe0UsA_6-V9j-u9G1qJW5-nhh1Nd1w6_gszmd-Jd7Pths7KaPxVyu-fwVGHBZgxB_AVymqd4</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Larsen, H A S</creator><creator>Austbø, L</creator><creator>Nødtvedt, A</creator><creator>Fraser, T W K</creator><creator>Rimstad, E</creator><creator>Fjelldal, P G</creator><creator>Hansen, T</creator><creator>Koppang, E O</creator><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>effect of vaccination, ploidy and smolt production regime on pathological melanin depositions in muscle tissue of Atlantic salmon, Salmo salar L</title><author>Larsen, H A S ; Austbø, L ; Nødtvedt, A ; Fraser, T W K ; Rimstad, E ; Fjelldal, P G ; Hansen, T ; Koppang, E O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f3466-fde4da4840a38cd9efd10ee3e11fa604e6933902e05cd20094ece15181b3295f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Aquaculture</topic><topic>diploid</topic><topic>Diploidy</topic><topic>farmed fish</topic><topic>fillets</topic><topic>Fish Diseases - genetics</topic><topic>Fish Diseases - pathology</topic><topic>fish industry</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>genes</topic><topic>Inflammation - etiology</topic><topic>Inflammation - genetics</topic><topic>Inflammation - pathology</topic><topic>Inflammation - veterinary</topic><topic>Marine</topic><topic>melanin</topic><topic>Melanins - metabolism</topic><topic>melanization</topic><topic>melanogenesis</topic><topic>melanomacrophage</topic><topic>muscle tissues</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - pathology</topic><topic>muscular diseases</topic><topic>Ploidies</topic><topic>Real-Time Polymerase Chain Reaction - veterinary</topic><topic>Salmo salar</topic><topic>smoltification</topic><topic>Temperature</topic><topic>triploid</topic><topic>Triploidy</topic><topic>vaccination</topic><topic>Vaccination - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Larsen, H A S</creatorcontrib><creatorcontrib>Austbø, L</creatorcontrib><creatorcontrib>Nødtvedt, A</creatorcontrib><creatorcontrib>Fraser, T W K</creatorcontrib><creatorcontrib>Rimstad, E</creatorcontrib><creatorcontrib>Fjelldal, P G</creatorcontrib><creatorcontrib>Hansen, T</creatorcontrib><creatorcontrib>Koppang, E O</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fish diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Larsen, H A S</au><au>Austbø, L</au><au>Nødtvedt, A</au><au>Fraser, T W K</au><au>Rimstad, E</au><au>Fjelldal, P G</au><au>Hansen, T</au><au>Koppang, E O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effect of vaccination, ploidy and smolt production regime on pathological melanin depositions in muscle tissue of Atlantic salmon, Salmo salar L</atitle><jtitle>Journal of fish diseases</jtitle><addtitle>J Fish Dis</addtitle><date>2014-04</date><risdate>2014</risdate><volume>37</volume><issue>4</issue><spage>327</spage><epage>340</epage><pages>327-340</pages><issn>0140-7775</issn><eissn>1365-2761</eissn><abstract>The presence of melanin in muscle fillets of farmed salmon represents a considerable quality problem for the salmon industry with major economic concerns. In this study, we have examined the presence of abnormal pigmentation in vaccinated versus unvaccinated Atlantic salmon, Salmo salar L., and evaluated possible differences between diploid and triploid fish. Furthermore, the impact of the smolt production regime at ambient (4.5 °C) versus elevated temperature (16 °C) was investigated. Pigmented muscle spots were analysed for the expression of genes involved in melanization (tyrosinase gene family) and immune‐related response in addition to morphological investigations. The proportion of fish with intramuscular melanin deposits was not significantly different between vaccinated and unvaccinated fish, regardless of ploidy. However, an interaction between vaccination and smolt regime was shown, where smoltification at elevated temperature after vaccination increased the number of affected individuals compared with vaccination followed by simulated natural smoltification. Furthermore, there were overall more fish with melanin spots amongst the triploids compared with their diploid counterparts. Transcription of the tyrosinase gene family confirmed an onsite melanogenesis in all pigment spots. The histological examination and the expression of the immune‐related genes revealed a chronic polyphasic myopathy that was not affected by vaccination, ploidy or smolt production regime.</abstract><cop>England</cop><pub>Blackwell Science</pub><pmid>23646928</pmid><doi>10.1111/jfd.12106</doi><tpages>14</tpages></addata></record> |
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| identifier | ISSN: 0140-7775 |
| ispartof | Journal of fish diseases, 2014-04, Vol.37 (4), p.327-340 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Aquaculture diploid Diploidy farmed fish fillets Fish Diseases - genetics Fish Diseases - pathology fish industry Fish Proteins - genetics Fish Proteins - metabolism genes Inflammation - etiology Inflammation - genetics Inflammation - pathology Inflammation - veterinary Marine melanin Melanins - metabolism melanization melanogenesis melanomacrophage muscle tissues Muscle, Skeletal - metabolism Muscle, Skeletal - pathology muscular diseases Ploidies Real-Time Polymerase Chain Reaction - veterinary Salmo salar smoltification Temperature triploid Triploidy vaccination Vaccination - adverse effects |
| title | effect of vaccination, ploidy and smolt production regime on pathological melanin depositions in muscle tissue of Atlantic salmon, Salmo salar L |
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