IFN‐τ Acts in a Dose‐Dependent Manner on Prostaglandin Production by Buffalo Endometrial Stromal Cells Cultured in vitro
Interferon‐τ (IFN‐τ) has been recognized as the primary embryonic signal responsible for maternal recognition of pregnancy. Uterine endometrium produces both prostaglandin F₂α (PGF₂α) and prostaglandin E₂ (PGE₂). PGF₂α is responsible for the luteolysis; however, PGE₂ favours establishment of pregnan...
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| Veröffentlicht in: | Reproduction in domestic animals 2014-06, Vol.49 (3), p.403-408 |
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| creator | Chethan, SG Singh, SK Nongsiej, J Rakesh, HB Singh, RP Kumar, N Agarwal, SK |
| description | Interferon‐τ (IFN‐τ) has been recognized as the primary embryonic signal responsible for maternal recognition of pregnancy. Uterine endometrium produces both prostaglandin F₂α (PGF₂α) and prostaglandin E₂ (PGE₂). PGF₂α is responsible for the luteolysis; however, PGE₂ favours establishment of pregnancy by its luteoprotective action. In this study, the dose‐response effect of recombinant bovine IFN‐τ (rbIFN‐τ) on prostaglandin (PG) production by buffalo endometrial stromal cells cultured in vitro was studied. Buffalo endometrial stromal cells were isolated by double enzymatic digestion, initially with trypsin III followed by a cocktail of trypsin III, collagenase type II and DNase I and subsequently cultured till confluence. Further, cells were treated with different doses of rbIFN‐τ (0.001, 0.01, 0.1, 1.0 and 10 μg/ml) and keeping a separate set of control. Culture supernatant was collected after 6, 12 and 24 h of treatment. PG levels in the culture supernatant were measured by enzyme immune assay (EIA) and total cellular protein estimated by Bradford method. Results indicated that buffalo endometrial stromal cells following rbIFN‐τ treatment enhanced the secretion of both PGE₂ and PGF₂α, and also its ratio in a strict dose‐dependent manner with a significant increase (p < 0.01) in PGE₂ production at 1 μg/ml dose of rbIFN‐τ and maximal stimulation for both PG was observed at 10 μg/ml. Further, both PG production and its ratio were increased significantly (p < 0.01) in a time‐dependent fashion in all the groups at 6, 12 and 24 h post‐treatment with highest level achieved at 24 h as compared with control. Absolute levels of PGE₂ remained higher than PGF₂α indicating PGE₂ as the major PG produced by endometrial stromal cells. The dose‐dependent response of rbIFN‐τ signifies the importance of optimum concentration of IFN‐τ for the embryonic development especially during the critical period to establish successful pregnancy. |
| doi_str_mv | 10.1111/rda.12287 |
| format | Article |
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Uterine endometrium produces both prostaglandin F₂α (PGF₂α) and prostaglandin E₂ (PGE₂). PGF₂α is responsible for the luteolysis; however, PGE₂ favours establishment of pregnancy by its luteoprotective action. In this study, the dose‐response effect of recombinant bovine IFN‐τ (rbIFN‐τ) on prostaglandin (PG) production by buffalo endometrial stromal cells cultured in vitro was studied. Buffalo endometrial stromal cells were isolated by double enzymatic digestion, initially with trypsin III followed by a cocktail of trypsin III, collagenase type II and DNase I and subsequently cultured till confluence. Further, cells were treated with different doses of rbIFN‐τ (0.001, 0.01, 0.1, 1.0 and 10 μg/ml) and keeping a separate set of control. Culture supernatant was collected after 6, 12 and 24 h of treatment. PG levels in the culture supernatant were measured by enzyme immune assay (EIA) and total cellular protein estimated by Bradford method. Results indicated that buffalo endometrial stromal cells following rbIFN‐τ treatment enhanced the secretion of both PGE₂ and PGF₂α, and also its ratio in a strict dose‐dependent manner with a significant increase (p < 0.01) in PGE₂ production at 1 μg/ml dose of rbIFN‐τ and maximal stimulation for both PG was observed at 10 μg/ml. Further, both PG production and its ratio were increased significantly (p < 0.01) in a time‐dependent fashion in all the groups at 6, 12 and 24 h post‐treatment with highest level achieved at 24 h as compared with control. Absolute levels of PGE₂ remained higher than PGF₂α indicating PGE₂ as the major PG produced by endometrial stromal cells. The dose‐dependent response of rbIFN‐τ signifies the importance of optimum concentration of IFN‐τ for the embryonic development especially during the critical period to establish successful pregnancy.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12287</identifier><identifier>PMID: 24612212</identifier><language>eng</language><publisher>Germany: Blackwell Science</publisher><subject>Animals ; Buffaloes ; Cattle ; Cells, Cultured ; collagenase ; cultured cells ; deoxyribonuclease I ; Dinoprost - analysis ; Dinoprost - biosynthesis ; Dinoprostone - analysis ; Dinoprostone - biosynthesis ; embryogenesis ; endometrium ; Endometrium - cytology ; Female ; Interferon Type I - pharmacology ; luteolysis ; Pregnancy ; Pregnancy Proteins - pharmacology ; prostaglandins ; Prostaglandins - biosynthesis ; Recombinant Proteins - pharmacology ; secretion ; stromal cells ; Stromal Cells - drug effects ; Stromal Cells - metabolism ; trypsin</subject><ispartof>Reproduction in domestic animals, 2014-06, Vol.49 (3), p.403-408</ispartof><rights>2014 Blackwell Verlag GmbH</rights><rights>2014 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3877-fc531324fa4c2213321720ee5c72de181c19f64ee62a1ad1358a96701d20b5f43</citedby><cites>FETCH-LOGICAL-c3877-fc531324fa4c2213321720ee5c72de181c19f64ee62a1ad1358a96701d20b5f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12287$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12287$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24612212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chethan, SG</creatorcontrib><creatorcontrib>Singh, SK</creatorcontrib><creatorcontrib>Nongsiej, J</creatorcontrib><creatorcontrib>Rakesh, HB</creatorcontrib><creatorcontrib>Singh, RP</creatorcontrib><creatorcontrib>Kumar, N</creatorcontrib><creatorcontrib>Agarwal, SK</creatorcontrib><title>IFN‐τ Acts in a Dose‐Dependent Manner on Prostaglandin Production by Buffalo Endometrial Stromal Cells Cultured in vitro</title><title>Reproduction in domestic animals</title><addtitle>Reprod Dom Anim</addtitle><description>Interferon‐τ (IFN‐τ) has been recognized as the primary embryonic signal responsible for maternal recognition of pregnancy. Uterine endometrium produces both prostaglandin F₂α (PGF₂α) and prostaglandin E₂ (PGE₂). PGF₂α is responsible for the luteolysis; however, PGE₂ favours establishment of pregnancy by its luteoprotective action. In this study, the dose‐response effect of recombinant bovine IFN‐τ (rbIFN‐τ) on prostaglandin (PG) production by buffalo endometrial stromal cells cultured in vitro was studied. Buffalo endometrial stromal cells were isolated by double enzymatic digestion, initially with trypsin III followed by a cocktail of trypsin III, collagenase type II and DNase I and subsequently cultured till confluence. Further, cells were treated with different doses of rbIFN‐τ (0.001, 0.01, 0.1, 1.0 and 10 μg/ml) and keeping a separate set of control. Culture supernatant was collected after 6, 12 and 24 h of treatment. PG levels in the culture supernatant were measured by enzyme immune assay (EIA) and total cellular protein estimated by Bradford method. Results indicated that buffalo endometrial stromal cells following rbIFN‐τ treatment enhanced the secretion of both PGE₂ and PGF₂α, and also its ratio in a strict dose‐dependent manner with a significant increase (p < 0.01) in PGE₂ production at 1 μg/ml dose of rbIFN‐τ and maximal stimulation for both PG was observed at 10 μg/ml. Further, both PG production and its ratio were increased significantly (p < 0.01) in a time‐dependent fashion in all the groups at 6, 12 and 24 h post‐treatment with highest level achieved at 24 h as compared with control. Absolute levels of PGE₂ remained higher than PGF₂α indicating PGE₂ as the major PG produced by endometrial stromal cells. The dose‐dependent response of rbIFN‐τ signifies the importance of optimum concentration of IFN‐τ for the embryonic development especially during the critical period to establish successful pregnancy.</description><subject>Animals</subject><subject>Buffaloes</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>collagenase</subject><subject>cultured cells</subject><subject>deoxyribonuclease I</subject><subject>Dinoprost - analysis</subject><subject>Dinoprost - biosynthesis</subject><subject>Dinoprostone - analysis</subject><subject>Dinoprostone - biosynthesis</subject><subject>embryogenesis</subject><subject>endometrium</subject><subject>Endometrium - cytology</subject><subject>Female</subject><subject>Interferon Type I - pharmacology</subject><subject>luteolysis</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - pharmacology</subject><subject>prostaglandins</subject><subject>Prostaglandins - biosynthesis</subject><subject>Recombinant Proteins - pharmacology</subject><subject>secretion</subject><subject>stromal cells</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - metabolism</subject><subject>trypsin</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9S1TAUxjOOjFzRhS-gWeqikD9t0y6vvYCMgIxXcJnJTU6ZaNtck1S9C2dc-AC8me_gkxgosCObM8n5nS_f-RB6QckuTWfPG7VLGavEIzSjOa8zUnD6GM1IzcusFGW1jZ6G8IUQWlRCPEHbLC8TT9kM_To6OP33--rvHzzXMWA7YIUXLkB6W8AaBgNDxCdqGMBjN-Az70JUl50ajL25mVFHmxqrDX47tq3qHN4fjOsheqs6vIze9ak20HUBN2MXRw_m-pvvNrWeoa00EuD5bd1B5wf7n5p32fGHw6NmfpxpngxnrU77cJa3KtfJNueMCkYACi2YAVpRTeu2zAFKpqgylBeVqktBqGFkVbQ530GvJ921d99GCFH2NujkSQ3gxiBpwWid0mJVQt9MqE6rBg-tXHvbK7-RlMjrtGVKW96kndiXt7LjqgdzT97Fm4C9CfhhO9g8rCQ_LuZ3ktk0YUOEn_cTyn-VpeCikJ9PDyU_uXjfXCxruUz8q4lvlZPq0tsgz5eM0JwQIgjnBf8PasWjZQ</recordid><startdate>201406</startdate><enddate>201406</enddate><creator>Chethan, SG</creator><creator>Singh, SK</creator><creator>Nongsiej, J</creator><creator>Rakesh, HB</creator><creator>Singh, RP</creator><creator>Kumar, N</creator><creator>Agarwal, SK</creator><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201406</creationdate><title>IFN‐τ Acts in a Dose‐Dependent Manner on Prostaglandin Production by Buffalo Endometrial Stromal Cells Cultured in vitro</title><author>Chethan, SG ; Singh, SK ; Nongsiej, J ; Rakesh, HB ; Singh, RP ; Kumar, N ; Agarwal, SK</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3877-fc531324fa4c2213321720ee5c72de181c19f64ee62a1ad1358a96701d20b5f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Buffaloes</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>collagenase</topic><topic>cultured cells</topic><topic>deoxyribonuclease I</topic><topic>Dinoprost - analysis</topic><topic>Dinoprost - biosynthesis</topic><topic>Dinoprostone - analysis</topic><topic>Dinoprostone - biosynthesis</topic><topic>embryogenesis</topic><topic>endometrium</topic><topic>Endometrium - cytology</topic><topic>Female</topic><topic>Interferon Type I - pharmacology</topic><topic>luteolysis</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - pharmacology</topic><topic>prostaglandins</topic><topic>Prostaglandins - biosynthesis</topic><topic>Recombinant Proteins - pharmacology</topic><topic>secretion</topic><topic>stromal cells</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - metabolism</topic><topic>trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chethan, SG</creatorcontrib><creatorcontrib>Singh, SK</creatorcontrib><creatorcontrib>Nongsiej, J</creatorcontrib><creatorcontrib>Rakesh, HB</creatorcontrib><creatorcontrib>Singh, RP</creatorcontrib><creatorcontrib>Kumar, N</creatorcontrib><creatorcontrib>Agarwal, SK</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chethan, SG</au><au>Singh, SK</au><au>Nongsiej, J</au><au>Rakesh, HB</au><au>Singh, RP</au><au>Kumar, N</au><au>Agarwal, SK</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IFN‐τ Acts in a Dose‐Dependent Manner on Prostaglandin Production by Buffalo Endometrial Stromal Cells Cultured in vitro</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Dom Anim</addtitle><date>2014-06</date><risdate>2014</risdate><volume>49</volume><issue>3</issue><spage>403</spage><epage>408</epage><pages>403-408</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Interferon‐τ (IFN‐τ) has been recognized as the primary embryonic signal responsible for maternal recognition of pregnancy. Uterine endometrium produces both prostaglandin F₂α (PGF₂α) and prostaglandin E₂ (PGE₂). PGF₂α is responsible for the luteolysis; however, PGE₂ favours establishment of pregnancy by its luteoprotective action. In this study, the dose‐response effect of recombinant bovine IFN‐τ (rbIFN‐τ) on prostaglandin (PG) production by buffalo endometrial stromal cells cultured in vitro was studied. Buffalo endometrial stromal cells were isolated by double enzymatic digestion, initially with trypsin III followed by a cocktail of trypsin III, collagenase type II and DNase I and subsequently cultured till confluence. Further, cells were treated with different doses of rbIFN‐τ (0.001, 0.01, 0.1, 1.0 and 10 μg/ml) and keeping a separate set of control. Culture supernatant was collected after 6, 12 and 24 h of treatment. PG levels in the culture supernatant were measured by enzyme immune assay (EIA) and total cellular protein estimated by Bradford method. Results indicated that buffalo endometrial stromal cells following rbIFN‐τ treatment enhanced the secretion of both PGE₂ and PGF₂α, and also its ratio in a strict dose‐dependent manner with a significant increase (p < 0.01) in PGE₂ production at 1 μg/ml dose of rbIFN‐τ and maximal stimulation for both PG was observed at 10 μg/ml. Further, both PG production and its ratio were increased significantly (p < 0.01) in a time‐dependent fashion in all the groups at 6, 12 and 24 h post‐treatment with highest level achieved at 24 h as compared with control. Absolute levels of PGE₂ remained higher than PGF₂α indicating PGE₂ as the major PG produced by endometrial stromal cells. The dose‐dependent response of rbIFN‐τ signifies the importance of optimum concentration of IFN‐τ for the embryonic development especially during the critical period to establish successful pregnancy.</abstract><cop>Germany</cop><pub>Blackwell Science</pub><pmid>24612212</pmid><doi>10.1111/rda.12287</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Buffaloes Cattle Cells, Cultured collagenase cultured cells deoxyribonuclease I Dinoprost - analysis Dinoprost - biosynthesis Dinoprostone - analysis Dinoprostone - biosynthesis embryogenesis endometrium Endometrium - cytology Female Interferon Type I - pharmacology luteolysis Pregnancy Pregnancy Proteins - pharmacology prostaglandins Prostaglandins - biosynthesis Recombinant Proteins - pharmacology secretion stromal cells Stromal Cells - drug effects Stromal Cells - metabolism trypsin |
| title | IFN‐τ Acts in a Dose‐Dependent Manner on Prostaglandin Production by Buffalo Endometrial Stromal Cells Cultured in vitro |
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