A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation
We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene ( dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin...
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| Veröffentlicht in: | Gene 1988-12, Vol.73 (2), p.531-535 |
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| container_end_page | 535 |
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| container_title | Gene |
| container_volume | 73 |
| creator | Parker, Breck Marinus, M.G. |
| description | We describe the isolation of a strain of
Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the
dam gene (
dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of
E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in
dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of
E. coli |
| doi_str_mv | 10.1016/0378-1119(88)90517-3 |
| format | Article |
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Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the
dam gene (
dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of
E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in
dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of
E. coli</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(88)90517-3</identifier><identifier>PMID: 2854098</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>bacteria ; Biological and medical sciences ; Chromosome Deletion ; Chromosome Mapping ; Chromosomes, Bacterial ; conjugation ; Crosses, Genetic ; DNA Transposable Elements ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Bacterial ; Genotype ; methylation ; Methyltransferases ; Molecular and cellular biology ; Molecular genetics ; Mutagenesis. Repair ; Mutation ; recombinant DNA ; Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics ; Transduction, Genetic</subject><ispartof>Gene, 1988-12, Vol.73 (2), p.531-535</ispartof><rights>1988</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-293c8729ca35c5bdcd4158bc6372337fe9a219f8edf1a1a2e8460609d4015bf13</citedby><cites>FETCH-LOGICAL-c483t-293c8729ca35c5bdcd4158bc6372337fe9a219f8edf1a1a2e8460609d4015bf13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(88)90517-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6575820$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2854098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parker, Breck</creatorcontrib><creatorcontrib>Marinus, M.G.</creatorcontrib><title>A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation</title><title>Gene</title><addtitle>Gene</addtitle><description>We describe the isolation of a strain of
Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the
dam gene (
dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of
E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in
dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of
E. coli</description><subject>bacteria</subject><subject>Biological and medical sciences</subject><subject>Chromosome Deletion</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Bacterial</subject><subject>conjugation</subject><subject>Crosses, Genetic</subject><subject>DNA Transposable Elements</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genotype</subject><subject>methylation</subject><subject>Methyltransferases</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutagenesis. Repair</subject><subject>Mutation</subject><subject>recombinant DNA</subject><subject>Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics</subject><subject>Transduction, Genetic</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1q3DAUhUVJSadp3yAFLUJpF270Y9lSFoUQ0h8IZJOuhSxdMwq2NdGVC9302WtnhoFsqo2EzncPl4-Qc86-cMabSyZbXXHOzSetPxumeFvJV2TDdWsqxqQ-IZsj8oa8RXxky1FKnJJToVXNjN6Qv9cU47gbgLop0Ox2MdARyjYFWhJNXXFxojh3WGKZS0wTHefi1gfSJblFv4Uc_TY66tMQr2jENDznNPXU0eBGGmCA9ecyTgj5Rck78rp3A8L7w31Gfn27fbj5Ud3df_95c31X-VrLUgkjvW6F8U4qr7rgQ82V7nwjWyFl24NxgpteQ-i5406ArhvWMBNqxlXXc3lGPu57dzk9zYDFjhE9DIObIM1ouRJcGdksYL0HfU6IGXq7y3F0-Y_lzK7a7erUrk6t1vZZu5XL2IdD_9yNEI5DB89LfnHIHXo39NlNPuIRa1SrtGAL9nWPweLid4Rs0UeYPISYwRcbUvz_Hv8AYxKf-w</recordid><startdate>19881220</startdate><enddate>19881220</enddate><creator>Parker, Breck</creator><creator>Marinus, M.G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19881220</creationdate><title>A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation</title><author>Parker, Breck ; Marinus, M.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-293c8729ca35c5bdcd4158bc6372337fe9a219f8edf1a1a2e8460609d4015bf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>bacteria</topic><topic>Biological and medical sciences</topic><topic>Chromosome Deletion</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Bacterial</topic><topic>conjugation</topic><topic>Crosses, Genetic</topic><topic>DNA Transposable Elements</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genotype</topic><topic>methylation</topic><topic>Methyltransferases</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutagenesis. Repair</topic><topic>Mutation</topic><topic>recombinant DNA</topic><topic>Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics</topic><topic>Transduction, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parker, Breck</creatorcontrib><creatorcontrib>Marinus, M.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parker, Breck</au><au>Marinus, M.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1988-12-20</date><risdate>1988</risdate><volume>73</volume><issue>2</issue><spage>531</spage><epage>535</epage><pages>531-535</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>We describe the isolation of a strain of
Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the
dam gene (
dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of
E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in
dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of
E. coli</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2854098</pmid><doi>10.1016/0378-1119(88)90517-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1988-12, Vol.73 (2), p.531-535 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | bacteria Biological and medical sciences Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial conjugation Crosses, Genetic DNA Transposable Elements Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Genotype methylation Methyltransferases Molecular and cellular biology Molecular genetics Mutagenesis. Repair Mutation recombinant DNA Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics Transduction, Genetic |
| title | A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation |
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