Controlled expression of heterologous cytochrome P450e cDNA in Saccharomyces cerevisiae. I. Construction and expression of a complete rat cytochrome P450e cDNA

A complete rat cytochrome P450e cDNA was constructed and placed between the yeast-inducible acid phosphatase (PHO5) promoter and terminator. This expression cassette was inserted into the yeast-replicating vector pDP34 which contains the entire yeast 2 μ plasmid element, the dLEU2 and the URA3 marke...

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Veröffentlicht in:	Journal of biotechnology 1989, Vol.9 (4), p.255-272
Hauptverfasser:	Zurbriggen, Beat, Böhlen, Elizabeth, Sanglard, Dominique, Käppeli, Othmar, Fiechter, Armin
Format:	Artikel
Sprache:	eng
Schlagworte:	cDNA cloning cloning vectors Complete cDNA Cytochrome P450e Deleted cDNA PHO5 expression system plasmids Saccharomyces cerevisiae
Online-Zugang:	Volltext
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