Optimal resolution of eye lens γ-crystallins by cation-exchange high-performance liquid chromatography on synchropak CM300
Cation-exchange high-performance liquid chromatography on SynChropak CM300 in Tris-acetate buffers of pH 5–7, using sodium acetate gradients, produces an excellent separation of the various γ-crystallin gene products and their post-synthetically modified forms from eye lens. With a single analysis o...
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| Veröffentlicht in: | Journal of Chromatography 1988-07, Vol.444, p.239-250 |
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| container_title | Journal of Chromatography |
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| creator | Siezen, Roland J. Kaplan, Elizabeth D. |
| description | Cation-exchange high-performance liquid chromatography on SynChropak CM300 in Tris-acetate buffers of pH 5–7, using sodium acetate gradients, produces an excellent separation of the various γ-crystallin gene products and their post-synthetically modified forms from eye lens. With a single analysis of total lens extract, the γ-crystallins can be resolved, quantified and collected for amino acid analysis.
Experimental conditions are presented for optimal resolution of individual human, rat, bovine and dogfish shark γ-crystallins. Applications presented include determinations of differntial synthesis of γ-crystallins and chemical modification (oxidation by hydrogen peroxide)
in situ. |
| doi_str_mv | 10.1016/S0021-9673(01)94027-0 |
| format | Article |
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Experimental conditions are presented for optimal resolution of individual human, rat, bovine and dogfish shark γ-crystallins. Applications presented include determinations of differntial synthesis of γ-crystallins and chemical modification (oxidation by hydrogen peroxide)
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Experimental conditions are presented for optimal resolution of individual human, rat, bovine and dogfish shark γ-crystallins. Applications presented include determinations of differntial synthesis of γ-crystallins and chemical modification (oxidation by hydrogen peroxide)
in situ.</description><subject>Aging - metabolism</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Cattle</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Crystallins - analysis</subject><subject>Dogfish</subject><subject>eye lens</subject><subject>gamma -crystallin</subject><subject>high-performance liquid chromatography</subject><subject>Humans</subject><subject>Infant</subject><subject>Ion Exchange Resins</subject><subject>Lens, Crystalline - analysis</subject><subject>Rats</subject><subject>Species Specificity</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1O5DAQhb1gBAxwBCSvRswiULaTTrJCo9bwI4FYAGvL7ZQ7ZpI42MmIiGNxD86EQ7fYsirVq_eqVB8hxwxOGbDF2T0AZ0m5yMUJsN9lCjxPYIfsf8l75GcITwAsh5zvkl3BIWUi3Sevd_1gW9VQj8E142BdR52hOCFtsAv0_S3RfgqDahob29VEtZpNCb7oWnVrpLVd10mP3jjfqk7HnH0ebUV17V2rBrf2qq8nGveGqZvFXv2jy1sBcEh-GNUEPNrWA_J48fdheZXc3F1eL__cJFosYEhMWhhkuqr0wqRZVZaAZZYzlqUrwwtuYJWboqi04KhYAQa1LoqsjLLIgCEXB-TXZm_v3fOIYZCtDRqbRnXoxiBZxpngoozGbGPU3oXg0cjeRzh-kgzkDFp-gpYzUQmxzqAlxNzx9sC4arH6Sm0px_n5Zo7xy_8WvQzaYmRVWY96kJWz31z4AFU4kic</recordid><startdate>19880701</startdate><enddate>19880701</enddate><creator>Siezen, Roland J.</creator><creator>Kaplan, Elizabeth D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope></search><sort><creationdate>19880701</creationdate><title>Optimal resolution of eye lens γ-crystallins by cation-exchange high-performance liquid chromatography on synchropak CM300</title><author>Siezen, Roland J. ; Kaplan, Elizabeth D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-f48fe1cddc6f45d990e9571154bf282f0b7f88dc32ea180fecc88590b73501e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Aging - metabolism</topic><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Cattle</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Crystallins - analysis</topic><topic>Dogfish</topic><topic>eye lens</topic><topic>gamma -crystallin</topic><topic>high-performance liquid chromatography</topic><topic>Humans</topic><topic>Infant</topic><topic>Ion Exchange Resins</topic><topic>Lens, Crystalline - analysis</topic><topic>Rats</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siezen, Roland J.</creatorcontrib><creatorcontrib>Kaplan, Elizabeth D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of Chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siezen, Roland J.</au><au>Kaplan, Elizabeth D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimal resolution of eye lens γ-crystallins by cation-exchange high-performance liquid chromatography on synchropak CM300</atitle><jtitle>Journal of Chromatography</jtitle><addtitle>J Chromatogr</addtitle><date>1988-07-01</date><risdate>1988</risdate><volume>444</volume><spage>239</spage><epage>250</epage><pages>239-250</pages><issn>0021-9673</issn><abstract>Cation-exchange high-performance liquid chromatography on SynChropak CM300 in Tris-acetate buffers of pH 5–7, using sodium acetate gradients, produces an excellent separation of the various γ-crystallin gene products and their post-synthetically modified forms from eye lens. With a single analysis of total lens extract, the γ-crystallins can be resolved, quantified and collected for amino acid analysis.
Experimental conditions are presented for optimal resolution of individual human, rat, bovine and dogfish shark γ-crystallins. Applications presented include determinations of differntial synthesis of γ-crystallins and chemical modification (oxidation by hydrogen peroxide)
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| ispartof | Journal of Chromatography, 1988-07, Vol.444, p.239-250 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aging - metabolism Amino Acids - analysis Animals Cattle Chromatography, High Pressure Liquid Chromatography, Ion Exchange Crystallins - analysis Dogfish eye lens gamma -crystallin high-performance liquid chromatography Humans Infant Ion Exchange Resins Lens, Crystalline - analysis Rats Species Specificity |
| title | Optimal resolution of eye lens γ-crystallins by cation-exchange high-performance liquid chromatography on synchropak CM300 |
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