Controlled expression of heterologous cytochrome P450e cDNA in Saccharomyces cerevisiae. II. Development of cultivation process for heterologous cytochrome P450e production

Cytochrome P450e was expressed in Saccharomyces cerevisiae under the regime of the PHO5 promoter and terminator. Derepression of the expression system was achieved by shifting the cells from C- to P-limited growth in a continuous culture. Alongside the production of heterologous cytochrome P450e, a...

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Veröffentlicht in:	Journal of biotechnology 1989, Vol.9 (4), p.273-285
Hauptverfasser:	Zurbriggen, Beat, Kühne, Anton B., Kallio, Pauli, Käppeli, Othmar, Fiechter, Armin
Format:	Artikel
Sprache:	eng
Schlagworte:	cDNA Continuous culture Controlled expression Cytochrome P450e gene expression PHO5 expression system Repeated fed-batch Saccharomyces cerevisiae
Online-Zugang:	Volltext
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