Bioluminescence flow system for determination of branched-chain l-amino acids in serum and urine
A continuous-flow bioluminescence method for determinations of branched-chain l-amino acids in serum and urine is described. Serum can be analyzed directly after simple filtration. Response is linear for 20-2000 pmol in the biological matrix. Leucine dehydrogenase is immobilized onto a nylon coil se...
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| Veröffentlicht in: | Analytica chimica acta 1988, Vol.205 (1-2), p.229-237 |
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| container_end_page | 237 |
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| container_issue | 1-2 |
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| container_title | Analytica chimica acta |
| container_volume | 205 |
| creator | Girotti, S. Roda, A. Angellotti, M.A. Ghini, S. Carrea, G. Bovara, R. Piazzi, S. Merighi, R. |
| description | A continuous-flow bioluminescence method for determinations of branched-chain
l-amino acids in serum and urine is described. Serum can be analyzed directly after simple filtration. Response is linear for 20-2000 pmol in the biological matrix. Leucine dehydrogenase is immobilized onto a nylon coil separated from the reactor coil containing bacterial bioluminescence enzymes. The stability of the immobilized is high (lifetime > two months) and more than 900 samples can be analyzed with the use of a few mg of enzymes. The results obtained agree well with those obtained by ion-exchange chromatography (amino acid analyzer). |
| doi_str_mv | 10.1016/S0003-2670(00)82332-6 |
| format | Article |
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l-amino acids in serum and urine is described. Serum can be analyzed directly after simple filtration. Response is linear for 20-2000 pmol in the biological matrix. Leucine dehydrogenase is immobilized onto a nylon coil separated from the reactor coil containing bacterial bioluminescence enzymes. The stability of the immobilized is high (lifetime > two months) and more than 900 samples can be analyzed with the use of a few mg of enzymes. The results obtained agree well with those obtained by ion-exchange chromatography (amino acid analyzer).</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/S0003-2670(00)82332-6</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>amino acids ; Analysis of complex biological substances ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Blood and biological fluids ; Fundamental and applied biological sciences. Psychology ; serum ; urine</subject><ispartof>Analytica chimica acta, 1988, Vol.205 (1-2), p.229-237</ispartof><rights>1988</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-e2ebcd7aeb3a405b0293d3f8c1bd99f5ed81c73e98fd2653db077122e3d7fdc93</citedby><cites>FETCH-LOGICAL-c370t-e2ebcd7aeb3a405b0293d3f8c1bd99f5ed81c73e98fd2653db077122e3d7fdc93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0003-2670(00)82332-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,777,781,786,787,3537,4010,4036,4037,23911,23912,25121,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19558532$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Girotti, S.</creatorcontrib><creatorcontrib>Roda, A.</creatorcontrib><creatorcontrib>Angellotti, M.A.</creatorcontrib><creatorcontrib>Ghini, S.</creatorcontrib><creatorcontrib>Carrea, G.</creatorcontrib><creatorcontrib>Bovara, R.</creatorcontrib><creatorcontrib>Piazzi, S.</creatorcontrib><creatorcontrib>Merighi, R.</creatorcontrib><title>Bioluminescence flow system for determination of branched-chain l-amino acids in serum and urine</title><title>Analytica chimica acta</title><description>A continuous-flow bioluminescence method for determinations of branched-chain
l-amino acids in serum and urine is described. Serum can be analyzed directly after simple filtration. Response is linear for 20-2000 pmol in the biological matrix. Leucine dehydrogenase is immobilized onto a nylon coil separated from the reactor coil containing bacterial bioluminescence enzymes. The stability of the immobilized is high (lifetime > two months) and more than 900 samples can be analyzed with the use of a few mg of enzymes. The results obtained agree well with those obtained by ion-exchange chromatography (amino acid analyzer).</description><subject>amino acids</subject><subject>Analysis of complex biological substances</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blood and biological fluids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>serum</subject><subject>urine</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQQK0KpG5pf0IlX0D0EBjb6zg5VQWVDwmJA_TsOvZYa5TEi5204t_X2UX0yGk0M29mNI-QrwzOGLD6_AEARMVrBacA3xouBK_qD2TFGiWqteDrA7J6Qz6STzk_lZQzWK_I74sQ-3kII2aLo0Xq-_iX5pc84UB9TNThhKn0zRTiSKOnXTKj3aCr7MaEkfaVKd1IjQ0u01LImOaBmtHROZW1n8mhN33GL6_xiPy6-vl4eVPd3V_fXv64q6xQMFXIsbNOGeyEWYPsgLfCCd9Y1rm29RJdw6wS2Dbe8VoK14FSjHMUTnlnW3FETvZ7tyk-z5gnPYTyUt-bEeOcNZMc2lbJAso9aFPMOaHX2xQGk140A7341DufepGlAfTOp67L3PHrAZOt6f2iIeT_w62UjRS8cN_3HJZv_wRMOtuwqHUhoZ20i-GdS_8ANCaLUQ</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Girotti, S.</creator><creator>Roda, A.</creator><creator>Angellotti, M.A.</creator><creator>Ghini, S.</creator><creator>Carrea, G.</creator><creator>Bovara, R.</creator><creator>Piazzi, S.</creator><creator>Merighi, R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>1988</creationdate><title>Bioluminescence flow system for determination of branched-chain l-amino acids in serum and urine</title><author>Girotti, S. ; Roda, A. ; Angellotti, M.A. ; Ghini, S. ; Carrea, G. ; Bovara, R. ; Piazzi, S. ; Merighi, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-e2ebcd7aeb3a405b0293d3f8c1bd99f5ed81c73e98fd2653db077122e3d7fdc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>amino acids</topic><topic>Analysis of complex biological substances</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blood and biological fluids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>serum</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Girotti, S.</creatorcontrib><creatorcontrib>Roda, A.</creatorcontrib><creatorcontrib>Angellotti, M.A.</creatorcontrib><creatorcontrib>Ghini, S.</creatorcontrib><creatorcontrib>Carrea, G.</creatorcontrib><creatorcontrib>Bovara, R.</creatorcontrib><creatorcontrib>Piazzi, S.</creatorcontrib><creatorcontrib>Merighi, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Girotti, S.</au><au>Roda, A.</au><au>Angellotti, M.A.</au><au>Ghini, S.</au><au>Carrea, G.</au><au>Bovara, R.</au><au>Piazzi, S.</au><au>Merighi, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioluminescence flow system for determination of branched-chain l-amino acids in serum and urine</atitle><jtitle>Analytica chimica acta</jtitle><date>1988</date><risdate>1988</risdate><volume>205</volume><issue>1-2</issue><spage>229</spage><epage>237</epage><pages>229-237</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>A continuous-flow bioluminescence method for determinations of branched-chain
l-amino acids in serum and urine is described. Serum can be analyzed directly after simple filtration. Response is linear for 20-2000 pmol in the biological matrix. Leucine dehydrogenase is immobilized onto a nylon coil separated from the reactor coil containing bacterial bioluminescence enzymes. The stability of the immobilized is high (lifetime > two months) and more than 900 samples can be analyzed with the use of a few mg of enzymes. The results obtained agree well with those obtained by ion-exchange chromatography (amino acid analyzer).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0003-2670(00)82332-6</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0003-2670 |
| ispartof | Analytica chimica acta, 1988, Vol.205 (1-2), p.229-237 |
| issn | 0003-2670 1873-4324 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15209975 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | amino acids Analysis of complex biological substances Analytical, structural and metabolic biochemistry Biological and medical sciences Blood and biological fluids Fundamental and applied biological sciences. Psychology serum urine |
| title | Bioluminescence flow system for determination of branched-chain l-amino acids in serum and urine |
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