Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks
Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The...
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| Veröffentlicht in: | Research in veterinary science 2013-06, Vol.94 (3), p.545-548 |
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| creator | Lawrence, J. Karpuzoglu, E. Vance, A. Vandenplas, M. Saba, C. Turek, M. Gogal, R.M. |
| description | Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX. |
| doi_str_mv | 10.1016/j.rvsc.2013.01.007 |
| format | Article |
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Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2013.01.007</identifier><identifier>PMID: 23375429</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibodies - immunology ; Blotting, Western - veterinary ; Cat Diseases - metabolism ; Cat Diseases - radiotherapy ; Cats ; Cell Line, Tumor ; Chemiluminescence ; DNA ; DNA Breaks, Double-Stranded - radiation effects ; DNA damage ; Feline ; Histones - biosynthesis ; Histones - immunology ; Histones - radiation effects ; Immunocytochemistry ; injection site ; mice ; monoclonal antibodies ; Reagent Kits, Diagnostic - veterinary ; sarcoma ; Sarcoma - metabolism ; Sarcoma - radiotherapy ; Sarcoma - veterinary ; Tumor ; Western Blot ; Western blotting</subject><ispartof>Research in veterinary science, 2013-06, Vol.94 (3), p.545-548</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-9c311d6fad2ad2cf4bd10d178231a1b3248d29b7b30efeafa61a5838075877cb3</citedby><cites>FETCH-LOGICAL-c413t-9c311d6fad2ad2cf4bd10d178231a1b3248d29b7b30efeafa61a5838075877cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S003452881300009X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23375429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawrence, J.</creatorcontrib><creatorcontrib>Karpuzoglu, E.</creatorcontrib><creatorcontrib>Vance, A.</creatorcontrib><creatorcontrib>Vandenplas, M.</creatorcontrib><creatorcontrib>Saba, C.</creatorcontrib><creatorcontrib>Turek, M.</creatorcontrib><creatorcontrib>Gogal, R.M.</creatorcontrib><title>Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Blotting, Western - veterinary</subject><subject>Cat Diseases - metabolism</subject><subject>Cat Diseases - radiotherapy</subject><subject>Cats</subject><subject>Cell Line, Tumor</subject><subject>Chemiluminescence</subject><subject>DNA</subject><subject>DNA Breaks, Double-Stranded - radiation effects</subject><subject>DNA damage</subject><subject>Feline</subject><subject>Histones - biosynthesis</subject><subject>Histones - immunology</subject><subject>Histones - radiation effects</subject><subject>Immunocytochemistry</subject><subject>injection site</subject><subject>mice</subject><subject>monoclonal antibodies</subject><subject>Reagent Kits, Diagnostic - veterinary</subject><subject>sarcoma</subject><subject>Sarcoma - metabolism</subject><subject>Sarcoma - radiotherapy</subject><subject>Sarcoma - veterinary</subject><subject>Tumor</subject><subject>Western Blot</subject><subject>Western blotting</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EokPhBViAl2wSfO04ThCb0fDTShUsoBI7y7Gvi4dMPNiZqjxX34NnwtEUlkWydCXr3E9X3yHkObAaGLSvt3W6zrbmDETNoGZMPSArkIJXvG3hIVkxJppK8q47IU9y3jLGGgD1mJxwIZRseL8i2813M11hpmGiv2-rM77-RvFmnzDnEKflN6RkXDAzOupxDBPSbJKNO0MtjmN-Q9cL5oI1c0w0euriYRgLNSczOfru05oOCc2P_JQ88mbM-OxunpLLD--_bs6qi88fzzfri8o2IOaqtwLAtd44Xp71zeCAOVAdF2BgELzpHO8HNQiGHo03LRjZiY4p2SllB3FKXh1z9yn-PGCe9S7k5VYzYTxkDZIz0fWNbP6PCi7F0pUqKD-iNsWcE3q9T2Fn0i8NTC869FYvOvSiQzPQRUdZenGXfxh26P6t_O2_AC-PgDdRm6sUsr78UhJkcdVzxpeIt0cCS2XXAZPONuBk0YWEdtYuhvsu-AP01KSX</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Lawrence, J.</creator><creator>Karpuzoglu, E.</creator><creator>Vance, A.</creator><creator>Vandenplas, M.</creator><creator>Saba, C.</creator><creator>Turek, M.</creator><creator>Gogal, R.M.</creator><general>Elsevier Ltd</general><general>Elsevier India Pvt Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20130601</creationdate><title>Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks</title><author>Lawrence, J. ; Karpuzoglu, E. ; Vance, A. ; Vandenplas, M. ; Saba, C. ; Turek, M. ; Gogal, R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-9c311d6fad2ad2cf4bd10d178231a1b3248d29b7b30efeafa61a5838075877cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Blotting, Western - veterinary</topic><topic>Cat Diseases - metabolism</topic><topic>Cat Diseases - radiotherapy</topic><topic>Cats</topic><topic>Cell Line, Tumor</topic><topic>Chemiluminescence</topic><topic>DNA</topic><topic>DNA Breaks, Double-Stranded - radiation effects</topic><topic>DNA damage</topic><topic>Feline</topic><topic>Histones - biosynthesis</topic><topic>Histones - immunology</topic><topic>Histones - radiation effects</topic><topic>Immunocytochemistry</topic><topic>injection site</topic><topic>mice</topic><topic>monoclonal antibodies</topic><topic>Reagent Kits, Diagnostic - veterinary</topic><topic>sarcoma</topic><topic>Sarcoma - metabolism</topic><topic>Sarcoma - radiotherapy</topic><topic>Sarcoma - veterinary</topic><topic>Tumor</topic><topic>Western Blot</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lawrence, J.</creatorcontrib><creatorcontrib>Karpuzoglu, E.</creatorcontrib><creatorcontrib>Vance, A.</creatorcontrib><creatorcontrib>Vandenplas, M.</creatorcontrib><creatorcontrib>Saba, C.</creatorcontrib><creatorcontrib>Turek, M.</creatorcontrib><creatorcontrib>Gogal, R.M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lawrence, J.</au><au>Karpuzoglu, E.</au><au>Vance, A.</au><au>Vandenplas, M.</au><au>Saba, C.</au><au>Turek, M.</au><au>Gogal, R.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>94</volume><issue>3</issue><spage>545</spage><epage>548</epage><pages>545-548</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23375429</pmid><doi>10.1016/j.rvsc.2013.01.007</doi><tpages>4</tpages></addata></record> |
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| ispartof | Research in veterinary science, 2013-06, Vol.94 (3), p.545-548 |
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| subjects | Animals Antibodies - immunology Blotting, Western - veterinary Cat Diseases - metabolism Cat Diseases - radiotherapy Cats Cell Line, Tumor Chemiluminescence DNA DNA Breaks, Double-Stranded - radiation effects DNA damage Feline Histones - biosynthesis Histones - immunology Histones - radiation effects Immunocytochemistry injection site mice monoclonal antibodies Reagent Kits, Diagnostic - veterinary sarcoma Sarcoma - metabolism Sarcoma - radiotherapy Sarcoma - veterinary Tumor Western Blot Western blotting |
| title | Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks |
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