Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines
Human milk oligosaccharides help to prevent infectious diseases in breastfed infants. Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2′-fucosyll...
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| Veröffentlicht in: | Nutrition research (New York, N.Y.) N.Y.), 2013-10, Vol.33 (10), p.831-838 |
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| creator | Weichert, Stefan Jennewein, Stefan Hüfner, Eric Weiss, Christel Borkowski, Julia Putze, Johannes Schroten, Horst |
| description | Human milk oligosaccharides help to prevent infectious diseases in breastfed infants. Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL) synthesized by whole-cell biocatalysis for their biological activity in vitro. Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2′-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 (reduction of 26%, 18%, 12%, and 17%, respectively), as could be shown for 3-FL (enteropathogenic E coli 29%, P aeruginosa 26%). Furthermore, adherence of P aeruginosa to the human respiratory epithelial cell line A549 was significantly inhibited by 2′-FL and 3-FL (reduction of 24% and 23%, respectively). These results confirm the biological and functional activity of biotechnologically synthesized human milk oligosaccharides. Mass-tailored human milk oligosaccharides could be used in the future to supplement infant formula ingredients or as preventatives to reduce the impact of infectious diseases. |
| doi_str_mv | 10.1016/j.nutres.2013.07.009 |
| format | Article |
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Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL) synthesized by whole-cell biocatalysis for their biological activity in vitro. Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2′-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 (reduction of 26%, 18%, 12%, and 17%, respectively), as could be shown for 3-FL (enteropathogenic E coli 29%, P aeruginosa 26%). Furthermore, adherence of P aeruginosa to the human respiratory epithelial cell line A549 was significantly inhibited by 2′-FL and 3-FL (reduction of 24% and 23%, respectively). These results confirm the biological and functional activity of biotechnologically synthesized human milk oligosaccharides. Mass-tailored human milk oligosaccharides could be used in the future to supplement infant formula ingredients or as preventatives to reduce the impact of infectious diseases.</description><identifier>ISSN: 0271-5317</identifier><identifier>EISSN: 1879-0739</identifier><identifier>DOI: 10.1016/j.nutres.2013.07.009</identifier><identifier>PMID: 24074741</identifier><identifier>CODEN: NTRSDC</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>2′-fucosyllactose ; 3′-fucosyllactose ; adhesion ; animal models ; Anti-Bacterial Agents - biosynthesis ; Anti-Bacterial Agents - pharmacology ; Bacterial adhesion ; Bacterial Adhesion - drug effects ; bioactive properties ; Biocatalysis ; Bioengineering ; Biological and medical sciences ; Breast Feeding ; breast milk ; Caco-2 Cells ; Campylobacter jejuni ; Campylobacter jejuni - drug effects ; Campylobacter jejuni - pathogenicity ; Cell culture techniques ; clinical trials ; enteropathogenic Escherichia coli ; Enteropathogenic Escherichia coli - drug effects ; Enteropathogenic Escherichia coli - pathogenicity ; Epithelial Cells - drug effects ; Epithelial Cells - microbiology ; Escherichia coli ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; Gastroenterology and Hepatology ; Gram-Negative Bacteria - drug effects ; Gram-Negative Bacteria - pathogenicity ; Human milk ; Humans ; infant formulas ; infants ; Infection - microbiology ; infectious diseases ; Intestines - drug effects ; Intestines - microbiology ; Milk, Human - chemistry ; oligosaccharides ; Oligosaccharides - biosynthesis ; Oligosaccharides - pharmacology ; pathogens ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - pathogenicity ; Respiratory System - drug effects ; Respiratory System - microbiology ; Salmonella enterica ; Salmonella enterica - drug effects ; Salmonella enterica - pathogenicity ; Trisaccharides - biosynthesis ; Trisaccharides - pharmacology ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Nutrition research (New York, N.Y.), 2013-10, Vol.33 (10), p.831-838</ispartof><rights>Elsevier Inc.</rights><rights>2013 Elsevier Inc.</rights><rights>2014 INIST-CNRS</rights><rights>2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-559eb65e19f1702d8ea9a7ba41b1f29ce60e1cb1f4e03e9a999599c50e7022453</citedby><cites>FETCH-LOGICAL-c570t-559eb65e19f1702d8ea9a7ba41b1f29ce60e1cb1f4e03e9a999599c50e7022453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0271531713001723$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27793098$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24074741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weichert, Stefan</creatorcontrib><creatorcontrib>Jennewein, Stefan</creatorcontrib><creatorcontrib>Hüfner, Eric</creatorcontrib><creatorcontrib>Weiss, Christel</creatorcontrib><creatorcontrib>Borkowski, Julia</creatorcontrib><creatorcontrib>Putze, Johannes</creatorcontrib><creatorcontrib>Schroten, Horst</creatorcontrib><title>Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines</title><title>Nutrition research (New York, N.Y.)</title><addtitle>Nutr Res</addtitle><description>Human milk oligosaccharides help to prevent infectious diseases in breastfed infants. Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL) synthesized by whole-cell biocatalysis for their biological activity in vitro. Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2′-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 (reduction of 26%, 18%, 12%, and 17%, respectively), as could be shown for 3-FL (enteropathogenic E coli 29%, P aeruginosa 26%). Furthermore, adherence of P aeruginosa to the human respiratory epithelial cell line A549 was significantly inhibited by 2′-FL and 3-FL (reduction of 24% and 23%, respectively). These results confirm the biological and functional activity of biotechnologically synthesized human milk oligosaccharides. Mass-tailored human milk oligosaccharides could be used in the future to supplement infant formula ingredients or as preventatives to reduce the impact of infectious diseases.</description><subject>2′-fucosyllactose</subject><subject>3′-fucosyllactose</subject><subject>adhesion</subject><subject>animal models</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial adhesion</subject><subject>Bacterial Adhesion - drug effects</subject><subject>bioactive properties</subject><subject>Biocatalysis</subject><subject>Bioengineering</subject><subject>Biological and medical sciences</subject><subject>Breast Feeding</subject><subject>breast milk</subject><subject>Caco-2 Cells</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - drug effects</subject><subject>Campylobacter jejuni - pathogenicity</subject><subject>Cell culture techniques</subject><subject>clinical trials</subject><subject>enteropathogenic Escherichia coli</subject><subject>Enteropathogenic Escherichia coli - drug effects</subject><subject>Enteropathogenic Escherichia coli - pathogenicity</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - microbiology</subject><subject>Escherichia coli</subject><subject>Feeding. Feeding behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastroenterology and Hepatology</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-Negative Bacteria - pathogenicity</subject><subject>Human milk</subject><subject>Humans</subject><subject>infant formulas</subject><subject>infants</subject><subject>Infection - microbiology</subject><subject>infectious diseases</subject><subject>Intestines - drug effects</subject><subject>Intestines - microbiology</subject><subject>Milk, Human - chemistry</subject><subject>oligosaccharides</subject><subject>Oligosaccharides - biosynthesis</subject><subject>Oligosaccharides - pharmacology</subject><subject>pathogens</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - pathogenicity</subject><subject>Respiratory System - drug effects</subject><subject>Respiratory System - microbiology</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - drug effects</subject><subject>Salmonella enterica - pathogenicity</subject><subject>Trisaccharides - biosynthesis</subject><subject>Trisaccharides - pharmacology</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0271-5317</issn><issn>1879-0739</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9uEzEQxlcIREPhDRD4gsRlw9hex-sLUqnKH6kSSKVny_HOJg4bO9hepNx4nh55JJ4EbxNA6qUnW9Zvvvk831TVcwpzCnTxZjP3Y46Y5gwon4OcA6gH1Yy2UtUguXpYzYBJWgtO5Un1JKUNAJWU88fVCWtANrKhs-rmnQvoV84jRuwI-_3zV92PNqT9MBibQ0JifEf43Ufn127pMsnrAnRrTC54EnryJeHYhW3wJhGDcSzKIZlbDfQZo7NkZ_I6rNAnkgNZj1vji1rGlJ03wy1ZfrVz0eQQ98TiMJCh-EtPq0e9GRI-O56n1fX7i6_nH-vLzx8-nZ9d1lZIyLUQCpcLgVT1VALrWjTKyKVp6JL2TFlcAFJb7g0CR2WUUkIpKwALzRrBT6vXB91dDN_H4ktvXZpsGI9hTJoKBryVi4bfjza8FVJAywraHFAbQ0oRe72LbmviXlPQU6B6ow-B6ilQDVKXQEvZi2OHcbnF7l_R3wQL8OoImGTN0EfjrUv_OSkVB9UW7uWB603QZhULc31VOjUAIKGhk8O3BwLLcH84jDpZh95i5yLarLvg7vN6V8CW4Fxx9Q33mDZhjCXhMhWdmAZ9Na3ntJ2UT5vJOP8DpjPjNQ</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Weichert, Stefan</creator><creator>Jennewein, Stefan</creator><creator>Hüfner, Eric</creator><creator>Weiss, Christel</creator><creator>Borkowski, Julia</creator><creator>Putze, Johannes</creator><creator>Schroten, Horst</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20131001</creationdate><title>Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines</title><author>Weichert, Stefan ; Jennewein, Stefan ; Hüfner, Eric ; Weiss, Christel ; Borkowski, Julia ; Putze, Johannes ; Schroten, Horst</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-559eb65e19f1702d8ea9a7ba41b1f29ce60e1cb1f4e03e9a999599c50e7022453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>2′-fucosyllactose</topic><topic>3′-fucosyllactose</topic><topic>adhesion</topic><topic>animal models</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial adhesion</topic><topic>Bacterial Adhesion - drug effects</topic><topic>bioactive properties</topic><topic>Biocatalysis</topic><topic>Bioengineering</topic><topic>Biological and medical sciences</topic><topic>Breast Feeding</topic><topic>breast milk</topic><topic>Caco-2 Cells</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - drug effects</topic><topic>Campylobacter jejuni - pathogenicity</topic><topic>Cell culture techniques</topic><topic>clinical trials</topic><topic>enteropathogenic Escherichia coli</topic><topic>Enteropathogenic Escherichia coli - drug effects</topic><topic>Enteropathogenic Escherichia coli - pathogenicity</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - microbiology</topic><topic>Escherichia coli</topic><topic>Feeding. Feeding behavior</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroenterology and Hepatology</topic><topic>Gram-Negative Bacteria - drug effects</topic><topic>Gram-Negative Bacteria - pathogenicity</topic><topic>Human milk</topic><topic>Humans</topic><topic>infant formulas</topic><topic>infants</topic><topic>Infection - microbiology</topic><topic>infectious diseases</topic><topic>Intestines - drug effects</topic><topic>Intestines - microbiology</topic><topic>Milk, Human - chemistry</topic><topic>oligosaccharides</topic><topic>Oligosaccharides - biosynthesis</topic><topic>Oligosaccharides - pharmacology</topic><topic>pathogens</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - pathogenicity</topic><topic>Respiratory System - drug effects</topic><topic>Respiratory System - microbiology</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica - drug effects</topic><topic>Salmonella enterica - pathogenicity</topic><topic>Trisaccharides - biosynthesis</topic><topic>Trisaccharides - pharmacology</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weichert, Stefan</creatorcontrib><creatorcontrib>Jennewein, Stefan</creatorcontrib><creatorcontrib>Hüfner, Eric</creatorcontrib><creatorcontrib>Weiss, Christel</creatorcontrib><creatorcontrib>Borkowski, Julia</creatorcontrib><creatorcontrib>Putze, Johannes</creatorcontrib><creatorcontrib>Schroten, Horst</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Nutrition research (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weichert, Stefan</au><au>Jennewein, Stefan</au><au>Hüfner, Eric</au><au>Weiss, Christel</au><au>Borkowski, Julia</au><au>Putze, Johannes</au><au>Schroten, Horst</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines</atitle><jtitle>Nutrition research (New York, N.Y.)</jtitle><addtitle>Nutr Res</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>33</volume><issue>10</issue><spage>831</spage><epage>838</epage><pages>831-838</pages><issn>0271-5317</issn><eissn>1879-0739</eissn><coden>NTRSDC</coden><abstract>Human milk oligosaccharides help to prevent infectious diseases in breastfed infants. Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL) synthesized by whole-cell biocatalysis for their biological activity in vitro. Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2′-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 (reduction of 26%, 18%, 12%, and 17%, respectively), as could be shown for 3-FL (enteropathogenic E coli 29%, P aeruginosa 26%). Furthermore, adherence of P aeruginosa to the human respiratory epithelial cell line A549 was significantly inhibited by 2′-FL and 3-FL (reduction of 24% and 23%, respectively). These results confirm the biological and functional activity of biotechnologically synthesized human milk oligosaccharides. Mass-tailored human milk oligosaccharides could be used in the future to supplement infant formula ingredients or as preventatives to reduce the impact of infectious diseases.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>24074741</pmid><doi>10.1016/j.nutres.2013.07.009</doi><tpages>8</tpages></addata></record> |
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| ispartof | Nutrition research (New York, N.Y.), 2013-10, Vol.33 (10), p.831-838 |
| issn | 0271-5317 1879-0739 |
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| subjects | 2′-fucosyllactose 3′-fucosyllactose adhesion animal models Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - pharmacology Bacterial adhesion Bacterial Adhesion - drug effects bioactive properties Biocatalysis Bioengineering Biological and medical sciences Breast Feeding breast milk Caco-2 Cells Campylobacter jejuni Campylobacter jejuni - drug effects Campylobacter jejuni - pathogenicity Cell culture techniques clinical trials enteropathogenic Escherichia coli Enteropathogenic Escherichia coli - drug effects Enteropathogenic Escherichia coli - pathogenicity Epithelial Cells - drug effects Epithelial Cells - microbiology Escherichia coli Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Gastroenterology and Hepatology Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - pathogenicity Human milk Humans infant formulas infants Infection - microbiology infectious diseases Intestines - drug effects Intestines - microbiology Milk, Human - chemistry oligosaccharides Oligosaccharides - biosynthesis Oligosaccharides - pharmacology pathogens Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - pathogenicity Respiratory System - drug effects Respiratory System - microbiology Salmonella enterica Salmonella enterica - drug effects Salmonella enterica - pathogenicity Trisaccharides - biosynthesis Trisaccharides - pharmacology Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines |
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