Thermostable Hemicellulases of a Bacterium, Geobacillus sp. DC3, Isolated from the Former Homestake Gold Mine in Lead, South Dakota
A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulase...
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| description | A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulases including β-xylosidase (0.209 U/mg) and arabinofuranosidase (0.230 U/mg), after the bacterium was grown in xylan for 24 h. Partially purified DC3 endoxylanase exhibited a molecular mass of approximately 43 kDa according to zymography with an optimal pH of 7 and optimal temperature of 70 °C. The kinetic constants, K ₘ and V ₘₐₓ, were 13.8 mg/mL and 77.5 μmol xylose/min·mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 °C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses. |
| doi_str_mv | 10.1007/s12010-014-0784-7 |
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The kinetic constants, K ₘ and V ₘₐₓ, were 13.8 mg/mL and 77.5 μmol xylose/min·mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 °C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-014-0784-7</identifier><identifier>PMID: 24549802</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Bacteria ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biochemistry ; Biological and medical sciences ; biomass ; Biomass energy ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Enzyme Stability ; Fundamental and applied biological sciences. Psychology ; Geobacillus ; Geobacillus - classification ; Geobacillus - enzymology ; Geobacillus - genetics ; Geobacillus - isolation & purification ; Geologic Sediments - microbiology ; Glycoside Hydrolases - chemistry ; Glycoside Hydrolases - genetics ; Glycoside Hydrolases - metabolism ; Gold ; Gold mines & mining ; Hydrogen-Ion Concentration ; hydrolysis ; industrial applications ; Microbiology ; Mining ; Molecular Sequence Data ; molecular weight ; Phylogeny ; South Dakota ; temperature ; thermal stability ; xylan 1,4-beta-xylosidase ; xylose</subject><ispartof>Applied biochemistry and biotechnology, 2014-04, Vol.172 (7), p.3488-3501</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-dfb7b810a5b105b3ad3ec6c1906d20c0ffef974d6f027847561c45191316f6563</citedby><cites>FETCH-LOGICAL-c525t-dfb7b810a5b105b3ad3ec6c1906d20c0ffef974d6f027847561c45191316f6563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-014-0784-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-014-0784-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28575610$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24549802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bergdale, Terran E</creatorcontrib><creatorcontrib>Hughes, Stephen R</creatorcontrib><creatorcontrib>Bang, Sookie S</creatorcontrib><title>Thermostable Hemicellulases of a Bacterium, Geobacillus sp. DC3, Isolated from the Former Homestake Gold Mine in Lead, South Dakota</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulases including β-xylosidase (0.209 U/mg) and arabinofuranosidase (0.230 U/mg), after the bacterium was grown in xylan for 24 h. Partially purified DC3 endoxylanase exhibited a molecular mass of approximately 43 kDa according to zymography with an optimal pH of 7 and optimal temperature of 70 °C. The kinetic constants, K ₘ and V ₘₐₓ, were 13.8 mg/mL and 77.5 μmol xylose/min·mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 °C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses.</description><subject>Bacteria</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>biomass</subject><subject>Biomass energy</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzyme Stability</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geobacillus</subject><subject>Geobacillus - classification</subject><subject>Geobacillus - enzymology</subject><subject>Geobacillus - genetics</subject><subject>Geobacillus - isolation & purification</subject><subject>Geologic Sediments - microbiology</subject><subject>Glycoside Hydrolases - chemistry</subject><subject>Glycoside Hydrolases - genetics</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>Gold</subject><subject>Gold mines & mining</subject><subject>Hydrogen-Ion Concentration</subject><subject>hydrolysis</subject><subject>industrial applications</subject><subject>Microbiology</subject><subject>Mining</subject><subject>Molecular Sequence Data</subject><subject>molecular weight</subject><subject>Phylogeny</subject><subject>South Dakota</subject><subject>temperature</subject><subject>thermal stability</subject><subject>xylan 1,4-beta-xylosidase</subject><subject>xylose</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU9v1DAQxS0EokvhA3ABSwiJw6bMOHGcHGFLdyst4tD2HDnOuJs2ibd2cuDMF8dLlj_igDj5ML_3PG8eYy8RzhBAvQ8oACEBzBJQRZaoR2yBUpYJiBIfswUIlSZCFOUJexbCHQCKQqqn7ERkMisLEAv27XpHvndh1HVHfEN9a6jrpk4HCtxZrvlHbUby7dQv-ZpcrU0b54GH_Rk_X6VLfhlcp0dquPWu5-OO-IXzPXm-cT1F33via9c1_HM7EG8HviXdLPmVm8YdP9f3btTP2ROru0Avju8pu7n4dL3aJNsv68vVh21ipJBj0tha1QWCljWCrFPdpGRygyXkjQAD1pItVdbkNuYuMiVzNJnEElPMbS7z9JS9m3333j1Mcbeqb8Mhrh7ITaFCKSBVpUD8DxRzJdKilBF98xd65yY_xCA_qOiYZQdDnCnjXQiebLX3ba_91wqhOpRZzWVWsczqUGaloubV0Xmqe2p-KX62F4G3R0AHozvr9WDa8JuLZccjQOTEzIU4Gm7J_7HiP35_PYusdpW-9dH45ipCGQAUssA8_Q77Wr0b</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Bergdale, Terran E</creator><creator>Hughes, Stephen R</creator><creator>Bang, Sookie S</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QO</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20140401</creationdate><title>Thermostable Hemicellulases of a Bacterium, Geobacillus sp. 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Psychology</topic><topic>Geobacillus</topic><topic>Geobacillus - classification</topic><topic>Geobacillus - enzymology</topic><topic>Geobacillus - genetics</topic><topic>Geobacillus - isolation & purification</topic><topic>Geologic Sediments - microbiology</topic><topic>Glycoside Hydrolases - chemistry</topic><topic>Glycoside Hydrolases - genetics</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>Gold</topic><topic>Gold mines & mining</topic><topic>Hydrogen-Ion Concentration</topic><topic>hydrolysis</topic><topic>industrial applications</topic><topic>Microbiology</topic><topic>Mining</topic><topic>Molecular Sequence Data</topic><topic>molecular weight</topic><topic>Phylogeny</topic><topic>South Dakota</topic><topic>temperature</topic><topic>thermal stability</topic><topic>xylan 1,4-beta-xylosidase</topic><topic>xylose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bergdale, Terran E</creatorcontrib><creatorcontrib>Hughes, Stephen R</creatorcontrib><creatorcontrib>Bang, Sookie S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bergdale, Terran E</au><au>Hughes, Stephen R</au><au>Bang, Sookie S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thermostable Hemicellulases of a Bacterium, Geobacillus sp. DC3, Isolated from the Former Homestake Gold Mine in Lead, South Dakota</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>172</volume><issue>7</issue><spage>3488</spage><epage>3501</epage><pages>3488-3501</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulases including β-xylosidase (0.209 U/mg) and arabinofuranosidase (0.230 U/mg), after the bacterium was grown in xylan for 24 h. Partially purified DC3 endoxylanase exhibited a molecular mass of approximately 43 kDa according to zymography with an optimal pH of 7 and optimal temperature of 70 °C. The kinetic constants, K ₘ and V ₘₐₓ, were 13.8 mg/mL and 77.5 μmol xylose/min·mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 °C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>24549802</pmid><doi>10.1007/s12010-014-0784-7</doi><tpages>14</tpages></addata></record> |
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| subjects | Bacteria Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biochemistry Biological and medical sciences biomass Biomass energy Biotechnology Chemistry Chemistry and Materials Science Enzyme Stability Fundamental and applied biological sciences. Psychology Geobacillus Geobacillus - classification Geobacillus - enzymology Geobacillus - genetics Geobacillus - isolation & purification Geologic Sediments - microbiology Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism Gold Gold mines & mining Hydrogen-Ion Concentration hydrolysis industrial applications Microbiology Mining Molecular Sequence Data molecular weight Phylogeny South Dakota temperature thermal stability xylan 1,4-beta-xylosidase xylose |
| title | Thermostable Hemicellulases of a Bacterium, Geobacillus sp. DC3, Isolated from the Former Homestake Gold Mine in Lead, South Dakota |
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