Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood
•Phenotypically distinct CD205Hi and CD205Lo myeloid DC subpopulations are present in bovine blood.•CD205Hi and CD205Lo myeloid DCs have an equal capacity to acquire antigen.•CD205Lo but not CD205Hi myeloid DCs efficiently activate T cell.•CD205Lo and CD205Hi myeloid DCs may represent developmentall...
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| Veröffentlicht in: | Developmental and comparative immunology 2014-06, Vol.44 (2), p.378-388 |
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| creator | González-Cano, Patricia Arsic, Natasa Popowych, Yurij I. Griebel, Philip J. |
| description | •Phenotypically distinct CD205Hi and CD205Lo myeloid DC subpopulations are present in bovine blood.•CD205Hi and CD205Lo myeloid DCs have an equal capacity to acquire antigen.•CD205Lo but not CD205Hi myeloid DCs efficiently activate T cell.•CD205Lo and CD205Hi myeloid DCs may represent developmentally distinct DC lineages.
Immature myeloid (m)DCs circulating in the blood of cattle have been defined as lineage negative (Lin−)MHCII+CD11c+CD205+ cells. Lin−MHCII+CD11c+CD205+ mDCs (0.2% blood mononuclear cells) isolated from bovine blood were heterogeneous in cell size and CD205 expression. Using highspeed cell sorting, Lin−MHCII+CD11c+CD205+ DCs were sorted into CD205Hi and CD205Lo subpopulations which were phenotypically distinct and differed significantly (P |
| doi_str_mv | 10.1016/j.dci.2014.01.014 |
| format | Article |
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Immature myeloid (m)DCs circulating in the blood of cattle have been defined as lineage negative (Lin−)MHCII+CD11c+CD205+ cells. Lin−MHCII+CD11c+CD205+ mDCs (0.2% blood mononuclear cells) isolated from bovine blood were heterogeneous in cell size and CD205 expression. Using highspeed cell sorting, Lin−MHCII+CD11c+CD205+ DCs were sorted into CD205Hi and CD205Lo subpopulations which were phenotypically distinct and differed significantly (P<0.01) in TLR gene expression. CD205Hi and CD205Lo mDCs were more efficient in macropinocytosis than monocytes and expressed no or little detectable non-specific esterase activity. CD205Lo mDCs efficiently activated purified allogeneic T cells and the addition of TLR agonists did not significantly alter this antigen presentation capacity. T cell activation by CD205Lo mDCs was associated with differential up-regulation of CD40, CD80, CD86 and TGFβ1 gene expression when compared to CD205Hi mDCs. In conclusion, two phenotypically and functionally distinct CD11c+CD205+ mDCs were isolated from blood that had an equal capacity to acquire antigen but markedly different capacities to activate T cells.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2014.01.014</identifier><identifier>PMID: 24502939</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Antigen Presentation ; Antigens, CD - metabolism ; Blood Circulation ; Bovine ; Cattle - immunology ; CD11c Antigen - metabolism ; Cell Differentiation ; Cell Lineage ; Cells, Cultured ; Co-stimulatory molecules ; Dendritic Cells - immunology ; Lectins, C-Type - metabolism ; Minor Histocompatibility Antigens ; Myeloid Cells - immunology ; Myeloid dendritic cells ; Receptors, Cell Surface - metabolism ; Toll-like receptor</subject><ispartof>Developmental and comparative immunology, 2014-06, Vol.44 (2), p.378-388</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-7f22aa423c226df3e7515dcc5f0ebac3ad71ea1a0547003a6e45ed93c1f12cf43</citedby><cites>FETCH-LOGICAL-c452t-7f22aa423c226df3e7515dcc5f0ebac3ad71ea1a0547003a6e45ed93c1f12cf43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2014.01.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24502939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>González-Cano, Patricia</creatorcontrib><creatorcontrib>Arsic, Natasa</creatorcontrib><creatorcontrib>Popowych, Yurij I.</creatorcontrib><creatorcontrib>Griebel, Philip J.</creatorcontrib><title>Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>•Phenotypically distinct CD205Hi and CD205Lo myeloid DC subpopulations are present in bovine blood.•CD205Hi and CD205Lo myeloid DCs have an equal capacity to acquire antigen.•CD205Lo but not CD205Hi myeloid DCs efficiently activate T cell.•CD205Lo and CD205Hi myeloid DCs may represent developmentally distinct DC lineages.
Immature myeloid (m)DCs circulating in the blood of cattle have been defined as lineage negative (Lin−)MHCII+CD11c+CD205+ cells. Lin−MHCII+CD11c+CD205+ mDCs (0.2% blood mononuclear cells) isolated from bovine blood were heterogeneous in cell size and CD205 expression. Using highspeed cell sorting, Lin−MHCII+CD11c+CD205+ DCs were sorted into CD205Hi and CD205Lo subpopulations which were phenotypically distinct and differed significantly (P<0.01) in TLR gene expression. CD205Hi and CD205Lo mDCs were more efficient in macropinocytosis than monocytes and expressed no or little detectable non-specific esterase activity. CD205Lo mDCs efficiently activated purified allogeneic T cells and the addition of TLR agonists did not significantly alter this antigen presentation capacity. T cell activation by CD205Lo mDCs was associated with differential up-regulation of CD40, CD80, CD86 and TGFβ1 gene expression when compared to CD205Hi mDCs. In conclusion, two phenotypically and functionally distinct CD11c+CD205+ mDCs were isolated from blood that had an equal capacity to acquire antigen but markedly different capacities to activate T cells.</description><subject>Animals</subject><subject>Antigen Presentation</subject><subject>Antigens, CD - metabolism</subject><subject>Blood Circulation</subject><subject>Bovine</subject><subject>Cattle - immunology</subject><subject>CD11c Antigen - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Cells, Cultured</subject><subject>Co-stimulatory molecules</subject><subject>Dendritic Cells - immunology</subject><subject>Lectins, C-Type - metabolism</subject><subject>Minor Histocompatibility Antigens</subject><subject>Myeloid Cells - immunology</subject><subject>Myeloid dendritic cells</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Toll-like receptor</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1rGzEQhkVpqR23P6CXoGMu64y-dr3kFELaBgy5uFDoQWilWZBZrzbSroP_fbXY6TF0GBgknnkZHkK-MVgzYOXtfu2sX3Ngcg0st_xAlmxT1QXApv5IlvlHFQLU7wW5SmkPuTYMPpMFlwp4Leol-bN7DbSdejv60JuuO1Hn0-jzmx5O2AXvqMPeRT96Sy12HU1TM4Rh6sy8kaiJSIeICfuR-p424eh7pE0XgvtCPrWmS_j1Mlfk1_fH3cPPYvv84-nhfltYqfhYVC3nxkguLOelawVWiilnrWoBG2OFcRVDwwwoWQEIU6JU6GphWcu4baVYkZtz7hDDy4Rp1Aef5ltNj2FKmikOotqUsvwPFEoJVSXqjLIzamNIKWKrh-gPJp40Az3r13ud9etZvwaWe77k-hI_NQd0_zbefGfg7gxg9nH0GHWyHnuLzke0o3bBvxP_F7dNlq8</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>González-Cano, Patricia</creator><creator>Arsic, Natasa</creator><creator>Popowych, Yurij I.</creator><creator>Griebel, Philip J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20140601</creationdate><title>Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood</title><author>González-Cano, Patricia ; Arsic, Natasa ; Popowych, Yurij I. ; Griebel, Philip J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-7f22aa423c226df3e7515dcc5f0ebac3ad71ea1a0547003a6e45ed93c1f12cf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antigen Presentation</topic><topic>Antigens, CD - metabolism</topic><topic>Blood Circulation</topic><topic>Bovine</topic><topic>Cattle - immunology</topic><topic>CD11c Antigen - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Lineage</topic><topic>Cells, Cultured</topic><topic>Co-stimulatory molecules</topic><topic>Dendritic Cells - immunology</topic><topic>Lectins, C-Type - metabolism</topic><topic>Minor Histocompatibility Antigens</topic><topic>Myeloid Cells - immunology</topic><topic>Myeloid dendritic cells</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Toll-like receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>González-Cano, Patricia</creatorcontrib><creatorcontrib>Arsic, Natasa</creatorcontrib><creatorcontrib>Popowych, Yurij I.</creatorcontrib><creatorcontrib>Griebel, Philip J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>González-Cano, Patricia</au><au>Arsic, Natasa</au><au>Popowych, Yurij I.</au><au>Griebel, Philip J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>44</volume><issue>2</issue><spage>378</spage><epage>388</epage><pages>378-388</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>•Phenotypically distinct CD205Hi and CD205Lo myeloid DC subpopulations are present in bovine blood.•CD205Hi and CD205Lo myeloid DCs have an equal capacity to acquire antigen.•CD205Lo but not CD205Hi myeloid DCs efficiently activate T cell.•CD205Lo and CD205Hi myeloid DCs may represent developmentally distinct DC lineages.
Immature myeloid (m)DCs circulating in the blood of cattle have been defined as lineage negative (Lin−)MHCII+CD11c+CD205+ cells. Lin−MHCII+CD11c+CD205+ mDCs (0.2% blood mononuclear cells) isolated from bovine blood were heterogeneous in cell size and CD205 expression. Using highspeed cell sorting, Lin−MHCII+CD11c+CD205+ DCs were sorted into CD205Hi and CD205Lo subpopulations which were phenotypically distinct and differed significantly (P<0.01) in TLR gene expression. CD205Hi and CD205Lo mDCs were more efficient in macropinocytosis than monocytes and expressed no or little detectable non-specific esterase activity. CD205Lo mDCs efficiently activated purified allogeneic T cells and the addition of TLR agonists did not significantly alter this antigen presentation capacity. T cell activation by CD205Lo mDCs was associated with differential up-regulation of CD40, CD80, CD86 and TGFβ1 gene expression when compared to CD205Hi mDCs. In conclusion, two phenotypically and functionally distinct CD11c+CD205+ mDCs were isolated from blood that had an equal capacity to acquire antigen but markedly different capacities to activate T cells.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>24502939</pmid><doi>10.1016/j.dci.2014.01.014</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Antigen Presentation Antigens, CD - metabolism Blood Circulation Bovine Cattle - immunology CD11c Antigen - metabolism Cell Differentiation Cell Lineage Cells, Cultured Co-stimulatory molecules Dendritic Cells - immunology Lectins, C-Type - metabolism Minor Histocompatibility Antigens Myeloid Cells - immunology Myeloid dendritic cells Receptors, Cell Surface - metabolism Toll-like receptor |
| title | Two functionally distinct myeloid dendritic cell subpopulations are present in bovine blood |
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