Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis
Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia an...
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Veröffentlicht in: | The veterinary journal (1997) 2013-12, Vol.198 (3), p.714-716 |
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container_title | The veterinary journal (1997) |
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creator | Heilmann, Romy M. Xenoulis, Panagiotis G. Barr, James W. Dowd, Scot E. Lawhon, Sara D. Suchodolski, Jan S. Steiner, Jörg M. |
description | Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis. |
doi_str_mv | 10.1016/j.tvjl.2013.10.001 |
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PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.</description><identifier>ISSN: 1090-0233</identifier><identifier>EISSN: 1532-2971</identifier><identifier>DOI: 10.1016/j.tvjl.2013.10.001</identifier><identifier>PMID: 24215778</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; bacteremia ; Bacteremia - diagnosis ; Bacteremia - microbiology ; Bacteremia - veterinary ; Bacterial DNA ; Bacteriological Techniques - methods ; Bacteriological Techniques - veterinary ; blood ; Blood culture ; Dog Diseases - diagnosis ; Dog Diseases - microbiology ; Dogs ; endocarditis ; Female ; hematologic tests ; humans ; Male ; Molecular technique ; pathogens ; PCR ; Pilot Projects ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; prognosis ; Sepsis ; Sepsis - diagnosis ; Sepsis - microbiology ; Sepsis - veterinary ; therapeutics</subject><ispartof>The veterinary journal (1997), 2013-12, Vol.198 (3), p.714-716</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</citedby><cites>FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tvjl.2013.10.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24215778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heilmann, Romy M.</creatorcontrib><creatorcontrib>Xenoulis, Panagiotis G.</creatorcontrib><creatorcontrib>Barr, James W.</creatorcontrib><creatorcontrib>Dowd, Scot E.</creatorcontrib><creatorcontrib>Lawhon, Sara D.</creatorcontrib><creatorcontrib>Suchodolski, Jan S.</creatorcontrib><creatorcontrib>Steiner, Jörg M.</creatorcontrib><title>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</title><title>The veterinary journal (1997)</title><addtitle>Vet J</addtitle><description>Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.</description><subject>Animals</subject><subject>bacteremia</subject><subject>Bacteremia - diagnosis</subject><subject>Bacteremia - microbiology</subject><subject>Bacteremia - veterinary</subject><subject>Bacterial DNA</subject><subject>Bacteriological Techniques - methods</subject><subject>Bacteriological Techniques - veterinary</subject><subject>blood</subject><subject>Blood culture</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>endocarditis</subject><subject>Female</subject><subject>hematologic tests</subject><subject>humans</subject><subject>Male</subject><subject>Molecular technique</subject><subject>pathogens</subject><subject>PCR</subject><subject>Pilot Projects</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>prognosis</subject><subject>Sepsis</subject><subject>Sepsis - diagnosis</subject><subject>Sepsis - microbiology</subject><subject>Sepsis - veterinary</subject><subject>therapeutics</subject><issn>1090-0233</issn><issn>1532-2971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3DAUhUVoaZJJ_0AWrZbdeKK3beimDGlTCKTksRaydJ1qsC1Xsickv74yM-0yWUnc-53D5RyEzilZU0LVxXY97bbdmhHK82BNCD1CJ1RyVrC6pO_yn9SkIIzzY3Sa0pYQUgvBPqBjJhiVZVmdILMJ_WiiT2HAocW_NrfYDA7bMOxgmHwYTIebLoQ8mrtpjoCnkAnTPb_AYdHG0GMXHhN-8tNvnOY0gp3A4QRj8ukMvW9Nl-Dj4V2hh--X95ur4vrmx8_Nt-vCCsqnoqyEssooIZmUTUUbU3Nnq9oyYLSmShrKnJSW8ZI5yzmtOMhGVVXFZcsl4Sv0Ze87xvBnhjTp3icLXWcGCHPSVDLCS84VexsVSohaEVlnlO1RG0NKEVo9Rt-b-Kwp0UsLequXFvTSwjLLLWTRp4P_3PTg_kv-xZ6Bz3ugNUGbxxy_frjLDjKrJV_OXKGvewJyZDsPUSfrYbDgfMzpahf8axf8BcMIoJ4</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Heilmann, Romy M.</creator><creator>Xenoulis, Panagiotis G.</creator><creator>Barr, James W.</creator><creator>Dowd, Scot E.</creator><creator>Lawhon, Sara D.</creator><creator>Suchodolski, Jan S.</creator><creator>Steiner, Jörg M.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20131201</creationdate><title>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</title><author>Heilmann, Romy M. ; Xenoulis, Panagiotis G. ; Barr, James W. ; Dowd, Scot E. ; Lawhon, Sara D. ; Suchodolski, Jan S. ; Steiner, Jörg M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>bacteremia</topic><topic>Bacteremia - diagnosis</topic><topic>Bacteremia - microbiology</topic><topic>Bacteremia - veterinary</topic><topic>Bacterial DNA</topic><topic>Bacteriological Techniques - methods</topic><topic>Bacteriological Techniques - veterinary</topic><topic>blood</topic><topic>Blood culture</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>endocarditis</topic><topic>Female</topic><topic>hematologic tests</topic><topic>humans</topic><topic>Male</topic><topic>Molecular technique</topic><topic>pathogens</topic><topic>PCR</topic><topic>Pilot Projects</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>prognosis</topic><topic>Sepsis</topic><topic>Sepsis - diagnosis</topic><topic>Sepsis - microbiology</topic><topic>Sepsis - veterinary</topic><topic>therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heilmann, Romy M.</creatorcontrib><creatorcontrib>Xenoulis, Panagiotis G.</creatorcontrib><creatorcontrib>Barr, James W.</creatorcontrib><creatorcontrib>Dowd, Scot E.</creatorcontrib><creatorcontrib>Lawhon, Sara D.</creatorcontrib><creatorcontrib>Suchodolski, Jan S.</creatorcontrib><creatorcontrib>Steiner, Jörg M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>The veterinary journal (1997)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heilmann, Romy M.</au><au>Xenoulis, Panagiotis G.</au><au>Barr, James W.</au><au>Dowd, Scot E.</au><au>Lawhon, Sara D.</au><au>Suchodolski, Jan S.</au><au>Steiner, Jörg M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</atitle><jtitle>The veterinary journal (1997)</jtitle><addtitle>Vet J</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>198</volume><issue>3</issue><spage>714</spage><epage>716</epage><pages>714-716</pages><issn>1090-0233</issn><eissn>1532-2971</eissn><abstract>Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>24215778</pmid><doi>10.1016/j.tvjl.2013.10.001</doi><tpages>3</tpages></addata></record> |
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subjects | Animals bacteremia Bacteremia - diagnosis Bacteremia - microbiology Bacteremia - veterinary Bacterial DNA Bacteriological Techniques - methods Bacteriological Techniques - veterinary blood Blood culture Dog Diseases - diagnosis Dog Diseases - microbiology Dogs endocarditis Female hematologic tests humans Male Molecular technique pathogens PCR Pilot Projects polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary prognosis Sepsis Sepsis - diagnosis Sepsis - microbiology Sepsis - veterinary therapeutics |
title | Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis |
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