Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis

Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia an...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The veterinary journal (1997) 2013-12, Vol.198 (3), p.714-716
Hauptverfasser: Heilmann, Romy M., Xenoulis, Panagiotis G., Barr, James W., Dowd, Scot E., Lawhon, Sara D., Suchodolski, Jan S., Steiner, Jörg M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 716
container_issue 3
container_start_page 714
container_title The veterinary journal (1997)
container_volume 198
creator Heilmann, Romy M.
Xenoulis, Panagiotis G.
Barr, James W.
Dowd, Scot E.
Lawhon, Sara D.
Suchodolski, Jan S.
Steiner, Jörg M.
description Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.
doi_str_mv 10.1016/j.tvjl.2013.10.001
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1520373362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1090023313005121</els_id><sourcerecordid>1464496059</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</originalsourceid><addsrcrecordid>eNqFkUtr3DAUhUVoaZJJ_0AWrZbdeKK3beimDGlTCKTksRaydJ1qsC1Xsickv74yM-0yWUnc-53D5RyEzilZU0LVxXY97bbdmhHK82BNCD1CJ1RyVrC6pO_yn9SkIIzzY3Sa0pYQUgvBPqBjJhiVZVmdILMJ_WiiT2HAocW_NrfYDA7bMOxgmHwYTIebLoQ8mrtpjoCnkAnTPb_AYdHG0GMXHhN-8tNvnOY0gp3A4QRj8ukMvW9Nl-Dj4V2hh--X95ur4vrmx8_Nt-vCCsqnoqyEssooIZmUTUUbU3Nnq9oyYLSmShrKnJSW8ZI5yzmtOMhGVVXFZcsl4Sv0Ze87xvBnhjTp3icLXWcGCHPSVDLCS84VexsVSohaEVlnlO1RG0NKEVo9Rt-b-Kwp0UsLequXFvTSwjLLLWTRp4P_3PTg_kv-xZ6Bz3ugNUGbxxy_frjLDjKrJV_OXKGvewJyZDsPUSfrYbDgfMzpahf8axf8BcMIoJ4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1464496059</pqid></control><display><type>article</type><title>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Heilmann, Romy M. ; Xenoulis, Panagiotis G. ; Barr, James W. ; Dowd, Scot E. ; Lawhon, Sara D. ; Suchodolski, Jan S. ; Steiner, Jörg M.</creator><creatorcontrib>Heilmann, Romy M. ; Xenoulis, Panagiotis G. ; Barr, James W. ; Dowd, Scot E. ; Lawhon, Sara D. ; Suchodolski, Jan S. ; Steiner, Jörg M.</creatorcontrib><description>Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.</description><identifier>ISSN: 1090-0233</identifier><identifier>EISSN: 1532-2971</identifier><identifier>DOI: 10.1016/j.tvjl.2013.10.001</identifier><identifier>PMID: 24215778</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; bacteremia ; Bacteremia - diagnosis ; Bacteremia - microbiology ; Bacteremia - veterinary ; Bacterial DNA ; Bacteriological Techniques - methods ; Bacteriological Techniques - veterinary ; blood ; Blood culture ; Dog Diseases - diagnosis ; Dog Diseases - microbiology ; Dogs ; endocarditis ; Female ; hematologic tests ; humans ; Male ; Molecular technique ; pathogens ; PCR ; Pilot Projects ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; prognosis ; Sepsis ; Sepsis - diagnosis ; Sepsis - microbiology ; Sepsis - veterinary ; therapeutics</subject><ispartof>The veterinary journal (1997), 2013-12, Vol.198 (3), p.714-716</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</citedby><cites>FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tvjl.2013.10.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24215778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heilmann, Romy M.</creatorcontrib><creatorcontrib>Xenoulis, Panagiotis G.</creatorcontrib><creatorcontrib>Barr, James W.</creatorcontrib><creatorcontrib>Dowd, Scot E.</creatorcontrib><creatorcontrib>Lawhon, Sara D.</creatorcontrib><creatorcontrib>Suchodolski, Jan S.</creatorcontrib><creatorcontrib>Steiner, Jörg M.</creatorcontrib><title>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</title><title>The veterinary journal (1997)</title><addtitle>Vet J</addtitle><description>Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.</description><subject>Animals</subject><subject>bacteremia</subject><subject>Bacteremia - diagnosis</subject><subject>Bacteremia - microbiology</subject><subject>Bacteremia - veterinary</subject><subject>Bacterial DNA</subject><subject>Bacteriological Techniques - methods</subject><subject>Bacteriological Techniques - veterinary</subject><subject>blood</subject><subject>Blood culture</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>endocarditis</subject><subject>Female</subject><subject>hematologic tests</subject><subject>humans</subject><subject>Male</subject><subject>Molecular technique</subject><subject>pathogens</subject><subject>PCR</subject><subject>Pilot Projects</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>prognosis</subject><subject>Sepsis</subject><subject>Sepsis - diagnosis</subject><subject>Sepsis - microbiology</subject><subject>Sepsis - veterinary</subject><subject>therapeutics</subject><issn>1090-0233</issn><issn>1532-2971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3DAUhUVoaZJJ_0AWrZbdeKK3beimDGlTCKTksRaydJ1qsC1Xsickv74yM-0yWUnc-53D5RyEzilZU0LVxXY97bbdmhHK82BNCD1CJ1RyVrC6pO_yn9SkIIzzY3Sa0pYQUgvBPqBjJhiVZVmdILMJ_WiiT2HAocW_NrfYDA7bMOxgmHwYTIebLoQ8mrtpjoCnkAnTPb_AYdHG0GMXHhN-8tNvnOY0gp3A4QRj8ukMvW9Nl-Dj4V2hh--X95ur4vrmx8_Nt-vCCsqnoqyEssooIZmUTUUbU3Nnq9oyYLSmShrKnJSW8ZI5yzmtOMhGVVXFZcsl4Sv0Ze87xvBnhjTp3icLXWcGCHPSVDLCS84VexsVSohaEVlnlO1RG0NKEVo9Rt-b-Kwp0UsLequXFvTSwjLLLWTRp4P_3PTg_kv-xZ6Bz3ugNUGbxxy_frjLDjKrJV_OXKGvewJyZDsPUSfrYbDgfMzpahf8axf8BcMIoJ4</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Heilmann, Romy M.</creator><creator>Xenoulis, Panagiotis G.</creator><creator>Barr, James W.</creator><creator>Dowd, Scot E.</creator><creator>Lawhon, Sara D.</creator><creator>Suchodolski, Jan S.</creator><creator>Steiner, Jörg M.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20131201</creationdate><title>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</title><author>Heilmann, Romy M. ; Xenoulis, Panagiotis G. ; Barr, James W. ; Dowd, Scot E. ; Lawhon, Sara D. ; Suchodolski, Jan S. ; Steiner, Jörg M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-7846c6a645255b81ba93dc89c2e219165a12d55c2372dc33183e5b688835f3503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>bacteremia</topic><topic>Bacteremia - diagnosis</topic><topic>Bacteremia - microbiology</topic><topic>Bacteremia - veterinary</topic><topic>Bacterial DNA</topic><topic>Bacteriological Techniques - methods</topic><topic>Bacteriological Techniques - veterinary</topic><topic>blood</topic><topic>Blood culture</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>endocarditis</topic><topic>Female</topic><topic>hematologic tests</topic><topic>humans</topic><topic>Male</topic><topic>Molecular technique</topic><topic>pathogens</topic><topic>PCR</topic><topic>Pilot Projects</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>prognosis</topic><topic>Sepsis</topic><topic>Sepsis - diagnosis</topic><topic>Sepsis - microbiology</topic><topic>Sepsis - veterinary</topic><topic>therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heilmann, Romy M.</creatorcontrib><creatorcontrib>Xenoulis, Panagiotis G.</creatorcontrib><creatorcontrib>Barr, James W.</creatorcontrib><creatorcontrib>Dowd, Scot E.</creatorcontrib><creatorcontrib>Lawhon, Sara D.</creatorcontrib><creatorcontrib>Suchodolski, Jan S.</creatorcontrib><creatorcontrib>Steiner, Jörg M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>The veterinary journal (1997)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heilmann, Romy M.</au><au>Xenoulis, Panagiotis G.</au><au>Barr, James W.</au><au>Dowd, Scot E.</au><au>Lawhon, Sara D.</au><au>Suchodolski, Jan S.</au><au>Steiner, Jörg M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis</atitle><jtitle>The veterinary journal (1997)</jtitle><addtitle>Vet J</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>198</volume><issue>3</issue><spage>714</spage><epage>716</epage><pages>714-716</pages><issn>1090-0233</issn><eissn>1532-2971</eissn><abstract>Sepsis carries a poor prognosis in critically ill dogs. PCR-based diagnostics could be more sensitive for detecting bacteremia than conventional blood culture, allowing earlier initiation of appropriate therapy. Molecular techniques have been considered as ancillary tools for detecting bacteremia and identifying pathogens in humans and in dogs with bacterial endocarditis. This study compared PCR analysis and blood culture for detecting bacteremia in six dogs with suspected sepsis and six healthy control dogs. One blood culture from a dog with suspected sepsis was positive but none from the healthy controls. PCR was negative for extracts from all dogs. This pilot study does not support the hypothesis that culture-independent PCR-based techniques used directly on small samples of blood are useful for diagnosing bacteremia in dogs with suspected sepsis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>24215778</pmid><doi>10.1016/j.tvjl.2013.10.001</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1090-0233
ispartof The veterinary journal (1997), 2013-12, Vol.198 (3), p.714-716
issn 1090-0233
1532-2971
language eng
recordid cdi_proquest_miscellaneous_1520373362
source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Animals
bacteremia
Bacteremia - diagnosis
Bacteremia - microbiology
Bacteremia - veterinary
Bacterial DNA
Bacteriological Techniques - methods
Bacteriological Techniques - veterinary
blood
Blood culture
Dog Diseases - diagnosis
Dog Diseases - microbiology
Dogs
endocarditis
Female
hematologic tests
humans
Male
Molecular technique
pathogens
PCR
Pilot Projects
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
prognosis
Sepsis
Sepsis - diagnosis
Sepsis - microbiology
Sepsis - veterinary
therapeutics
title Comparison of PCR and conventional blood culture to analyze blood from dogs with suspected sepsis
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T19%3A15%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20PCR%20and%20conventional%20blood%20culture%20to%20analyze%20blood%20from%20dogs%20with%20suspected%20sepsis&rft.jtitle=The%20veterinary%20journal%20(1997)&rft.au=Heilmann,%20Romy%20M.&rft.date=2013-12-01&rft.volume=198&rft.issue=3&rft.spage=714&rft.epage=716&rft.pages=714-716&rft.issn=1090-0233&rft.eissn=1532-2971&rft_id=info:doi/10.1016/j.tvjl.2013.10.001&rft_dat=%3Cproquest_cross%3E1464496059%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1464496059&rft_id=info:pmid/24215778&rft_els_id=S1090023313005121&rfr_iscdi=true