Transient expression of VP2 in Nicotiana benthamiana and its use as a plant-based vaccine against Infectious Bursal Disease Virus

Highlights • IBDV's VP2 was expressed in N. benthamiana retaining its immunogenicity. • Immunized chickens produced humoral immune response. • Intramuscular inoculation of chickens elicits neutralizing antibodies.

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Bibliographische Detailangaben
Veröffentlicht in:	Vaccine 2013-05, Vol.31 (23), p.2623-2627
Hauptverfasser:	Gómez, Evangelina, Lucero, María Soledad, Chimeno Zoth, Silvina, Carballeda, Juan Manuel, Gravisaco, María José, Berinstein, Analía
Format:	Artikel
Sprache:	eng
Schlagworte:	agroinfiltration Allergy and Immunology Animals Antibodies, Neutralizing - biosynthesis Antibodies, Neutralizing - immunology Applied microbiology Biological and medical sciences Birnaviridae Infections - immunology Birnaviridae Infections - prevention & control Birnaviridae Infections - veterinary Chick Embryo Chickens Economics epitopes etiological agents Fundamental and applied biological sciences. Psychology humoral immunity IBDV Immune response immunosuppressive agents Infectious bursal disease virus Infectious bursal disease virus - genetics Infectious bursal disease virus - immunology leaves Microbiology Miscellaneous neutralization Nicotiana - genetics Nicotiana - immunology Nicotiana - metabolism Nicotiana - microbiology Nicotiana benthamiana Plant expression Poultry Diseases - immunology Poultry Diseases - prevention & control Poultry Diseases - virology poultry industry Recombinant vaccines subunit vaccines T-Lymphocytes - immunology Vaccination - veterinary Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Subunit - biosynthesis Vaccines, Subunit - immunology Vaccines, Synthetic - biosynthesis Vaccines, Synthetic - immunology Veterinary antigens Viral Structural Proteins - biosynthesis Viral Structural Proteins - genetics Viral Structural Proteins - immunology Viral Vaccines - biosynthesis Viral Vaccines - immunology Virology
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Beschreibung
Zusammenfassung:	Highlights • IBDV's VP2 was expressed in N. benthamiana retaining its immunogenicity. • Immunized chickens produced humoral immune response. • Intramuscular inoculation of chickens elicits neutralizing antibodies.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2013.03.064