Identification and characterization of a basic thaumatin‐like protein (TLP 2) as an allergen in sapodilla plum (Manilkara zapota)
SCOPE: Cases of oral allergy syndrome following the ingestion of sapodilla plum (Manilkara zapota) have been reported rarely. As the causative allergens are not known, the main objective of this study was to identify and characterize the important allergens in sapodilla. METHODS AND RESULTS: Allergy...
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Veröffentlicht in: | Molecular nutrition & food research 2014-04, Vol.58 (4), p.894-902 |
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description | SCOPE: Cases of oral allergy syndrome following the ingestion of sapodilla plum (Manilkara zapota) have been reported rarely. As the causative allergens are not known, the main objective of this study was to identify and characterize the important allergens in sapodilla. METHODS AND RESULTS: Allergy to sapodilla was diagnosed by case history, skin prick test, and serum allergen–specific IgE. The allergen was detected by IgE immunoblotting, purified on SP–Sepharose and characterized by native/SDS–PAGE, IEF, MS, and amino acid composition. Several cases of allergy to sapodilla fruit were identified; majority of the sapodilla‐allergic subjects (6/7) experienced typical oral allergy syndrome symptoms, and allergen–specific IgE to the purified protein was positive. The allergen has a pI ≥9.5 and high contents of arginine, threonine, glycine, and cysteine. Circular dichroism revealed a secondary structure rich in beta sheets/turns. Based on its N‐terminal sequence of A‐T‐F‐D‐I‐Q‐N‐N‐C‐X‐Y‐, the allergen (21 578 Da) was identified as a thaumatin‐like protein by homology. CONCLUSION: The causative allergen in sapodilla plum has been identified and characterized as a highly basic thaumatin‐like protein belonging to the pathogenesis‐related protein (PR–5) family, which has been recognized as a new family of conserved, cross‐reactive plant allergens. |
doi_str_mv | 10.1002/mnfr.201300261 |
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As the causative allergens are not known, the main objective of this study was to identify and characterize the important allergens in sapodilla. METHODS AND RESULTS: Allergy to sapodilla was diagnosed by case history, skin prick test, and serum allergen–specific IgE. The allergen was detected by IgE immunoblotting, purified on SP–Sepharose and characterized by native/SDS–PAGE, IEF, MS, and amino acid composition. Several cases of allergy to sapodilla fruit were identified; majority of the sapodilla‐allergic subjects (6/7) experienced typical oral allergy syndrome symptoms, and allergen–specific IgE to the purified protein was positive. The allergen has a pI ≥9.5 and high contents of arginine, threonine, glycine, and cysteine. Circular dichroism revealed a secondary structure rich in beta sheets/turns. Based on its N‐terminal sequence of A‐T‐F‐D‐I‐Q‐N‐N‐C‐X‐Y‐, the allergen (21 578 Da) was identified as a thaumatin‐like protein by homology. CONCLUSION: The causative allergen in sapodilla plum has been identified and characterized as a highly basic thaumatin‐like protein belonging to the pathogenesis‐related protein (PR–5) family, which has been recognized as a new family of conserved, cross‐reactive plant allergens.</description><identifier>ISSN: 1613-4125</identifier><identifier>EISSN: 1613-4133</identifier><identifier>DOI: 10.1002/mnfr.201300261</identifier><identifier>PMID: 24311523</identifier><language>eng</language><publisher>Weinheim: Blackwell Publishing Ltd</publisher><subject>allergens ; amino acid composition ; Antigens, Plant - immunology ; Antigens, Plant - isolation & purification ; arginine ; Arginine-rich protein ; Biological and medical sciences ; Cross Reactions ; cysteine ; Electrophoresis, Polyacrylamide Gel ; Feeding. Feeding behavior ; Food Hypersensitivity - immunology ; Fruit allergen ; Fundamental and applied biological sciences. Psychology ; Humans ; hypersensitivity ; immunoblotting ; Immunoglobulin E ; ingestion ; Manilkara ; Manilkara zapota ; new family ; OAS ; Plant Proteins - immunology ; Prunus ; Sapodilla ; sapodillas ; Sequence Homology, Amino Acid ; Skin Tests ; Thaumatin-like protein ; threonine ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Molecular nutrition & food research, 2014-04, Vol.58 (4), p.894-902</ispartof><rights>2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>2013 WILEY-VCH Verlag GmbH & Co. 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Nutr. Food Res</addtitle><description>SCOPE: Cases of oral allergy syndrome following the ingestion of sapodilla plum (Manilkara zapota) have been reported rarely. As the causative allergens are not known, the main objective of this study was to identify and characterize the important allergens in sapodilla. METHODS AND RESULTS: Allergy to sapodilla was diagnosed by case history, skin prick test, and serum allergen–specific IgE. The allergen was detected by IgE immunoblotting, purified on SP–Sepharose and characterized by native/SDS–PAGE, IEF, MS, and amino acid composition. Several cases of allergy to sapodilla fruit were identified; majority of the sapodilla‐allergic subjects (6/7) experienced typical oral allergy syndrome symptoms, and allergen–specific IgE to the purified protein was positive. The allergen has a pI ≥9.5 and high contents of arginine, threonine, glycine, and cysteine. Circular dichroism revealed a secondary structure rich in beta sheets/turns. Based on its N‐terminal sequence of A‐T‐F‐D‐I‐Q‐N‐N‐C‐X‐Y‐, the allergen (21 578 Da) was identified as a thaumatin‐like protein by homology. CONCLUSION: The causative allergen in sapodilla plum has been identified and characterized as a highly basic thaumatin‐like protein belonging to the pathogenesis‐related protein (PR–5) family, which has been recognized as a new family of conserved, cross‐reactive plant allergens.</description><subject>allergens</subject><subject>amino acid composition</subject><subject>Antigens, Plant - immunology</subject><subject>Antigens, Plant - isolation & purification</subject><subject>arginine</subject><subject>Arginine-rich protein</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>cysteine</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Feeding. Feeding behavior</subject><subject>Food Hypersensitivity - immunology</subject><subject>Fruit allergen</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>hypersensitivity</subject><subject>immunoblotting</subject><subject>Immunoglobulin E</subject><subject>ingestion</subject><subject>Manilkara</subject><subject>Manilkara zapota</subject><subject>new family</subject><subject>OAS</subject><subject>Plant Proteins - immunology</subject><subject>Prunus</subject><subject>Sapodilla</subject><subject>sapodillas</subject><subject>Sequence Homology, Amino Acid</subject><subject>Skin Tests</subject><subject>Thaumatin-like protein</subject><subject>threonine</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>1613-4125</issn><issn>1613-4133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcluFDEQhlsIRELgyhF8QZocenB56eWIIjJEmgkhJOJoVbvtxEwvg90tSE5IvADPyJPgUQ_DES5eqr7_r1JVkjwHOgdK2eu2s37OKPD4yeBBcggZ8FQA5w_3byYPkichfKaUAxP8cXIQTwDJ-GHy46w23eCs0zi4viPY1UTfokc9GO_up2BvCZIKg9NkuMWxjdHu1_efjVsbsvH9YFxHZlfLC8KOCYboQbBpjL8xHYmZgJu-dk2DZNOMLZmtsHPNOpYg9zEz4PHT5JHFJphnu_souT59e3XyLl2-X5ydvFmmWmSSpVktMKugllBUArRgguW50bSCwnIp8toWIAuKAouihgxLaSnWNs8l1RR0zY-S2eQbe_4ymjCo1gVtYmed6ceg4kQoz0DK_D_QOFUpeQkRnU-o9n0I3li18a5Ff6eAqu2O1HZHar-jKHix8x6r1tR7_M9SIvBqB2DQ2FiPnXbhL1fwknEoIycm7qtrzN0_yqrV-eklA8aiLJ1kLgzm216Gfq2ynOdSfTpfqNWHsrzgi0u1ncXLibfYK7zxsZXrj9FXUEpzmhcl_w1i7MI8</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Hegde, Venkatesh L</creator><creator>Ashok Kumar, Hassan G</creator><creator>Sreenath, Kundimi</creator><creator>Hegde, Muralidhar L</creator><creator>Venkatesh, Yeldur P</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201404</creationdate><title>Identification and characterization of a basic thaumatin‐like protein (TLP 2) as an allergen in sapodilla plum (Manilkara zapota)</title><author>Hegde, Venkatesh L ; Ashok Kumar, Hassan G ; Sreenath, Kundimi ; Hegde, Muralidhar L ; Venkatesh, Yeldur P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4652-6d4a6b1d518b41c424277ec0b18f3547df81580a4a88d16a95f0adf7750c01cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>allergens</topic><topic>amino acid composition</topic><topic>Antigens, Plant - immunology</topic><topic>Antigens, Plant - isolation & purification</topic><topic>arginine</topic><topic>Arginine-rich protein</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>cysteine</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Feeding. Feeding behavior</topic><topic>Food Hypersensitivity - immunology</topic><topic>Fruit allergen</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>hypersensitivity</topic><topic>immunoblotting</topic><topic>Immunoglobulin E</topic><topic>ingestion</topic><topic>Manilkara</topic><topic>Manilkara zapota</topic><topic>new family</topic><topic>OAS</topic><topic>Plant Proteins - immunology</topic><topic>Prunus</topic><topic>Sapodilla</topic><topic>sapodillas</topic><topic>Sequence Homology, Amino Acid</topic><topic>Skin Tests</topic><topic>Thaumatin-like protein</topic><topic>threonine</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hegde, Venkatesh L</creatorcontrib><creatorcontrib>Ashok Kumar, Hassan G</creatorcontrib><creatorcontrib>Sreenath, Kundimi</creatorcontrib><creatorcontrib>Hegde, Muralidhar L</creatorcontrib><creatorcontrib>Venkatesh, Yeldur P</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Molecular nutrition & food research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hegde, Venkatesh L</au><au>Ashok Kumar, Hassan G</au><au>Sreenath, Kundimi</au><au>Hegde, Muralidhar L</au><au>Venkatesh, Yeldur P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a basic thaumatin‐like protein (TLP 2) as an allergen in sapodilla plum (Manilkara zapota)</atitle><jtitle>Molecular nutrition & food research</jtitle><addtitle>Mol. Nutr. Food Res</addtitle><date>2014-04</date><risdate>2014</risdate><volume>58</volume><issue>4</issue><spage>894</spage><epage>902</epage><pages>894-902</pages><issn>1613-4125</issn><eissn>1613-4133</eissn><abstract>SCOPE: Cases of oral allergy syndrome following the ingestion of sapodilla plum (Manilkara zapota) have been reported rarely. As the causative allergens are not known, the main objective of this study was to identify and characterize the important allergens in sapodilla. METHODS AND RESULTS: Allergy to sapodilla was diagnosed by case history, skin prick test, and serum allergen–specific IgE. The allergen was detected by IgE immunoblotting, purified on SP–Sepharose and characterized by native/SDS–PAGE, IEF, MS, and amino acid composition. Several cases of allergy to sapodilla fruit were identified; majority of the sapodilla‐allergic subjects (6/7) experienced typical oral allergy syndrome symptoms, and allergen–specific IgE to the purified protein was positive. The allergen has a pI ≥9.5 and high contents of arginine, threonine, glycine, and cysteine. Circular dichroism revealed a secondary structure rich in beta sheets/turns. Based on its N‐terminal sequence of A‐T‐F‐D‐I‐Q‐N‐N‐C‐X‐Y‐, the allergen (21 578 Da) was identified as a thaumatin‐like protein by homology. CONCLUSION: The causative allergen in sapodilla plum has been identified and characterized as a highly basic thaumatin‐like protein belonging to the pathogenesis‐related protein (PR–5) family, which has been recognized as a new family of conserved, cross‐reactive plant allergens.</abstract><cop>Weinheim</cop><pub>Blackwell Publishing Ltd</pub><pmid>24311523</pmid><doi>10.1002/mnfr.201300261</doi><tpages>9</tpages></addata></record> |
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subjects | allergens amino acid composition Antigens, Plant - immunology Antigens, Plant - isolation & purification arginine Arginine-rich protein Biological and medical sciences Cross Reactions cysteine Electrophoresis, Polyacrylamide Gel Feeding. Feeding behavior Food Hypersensitivity - immunology Fruit allergen Fundamental and applied biological sciences. Psychology Humans hypersensitivity immunoblotting Immunoglobulin E ingestion Manilkara Manilkara zapota new family OAS Plant Proteins - immunology Prunus Sapodilla sapodillas Sequence Homology, Amino Acid Skin Tests Thaumatin-like protein threonine Vertebrates: anatomy and physiology, studies on body, several organs or systems |
title | Identification and characterization of a basic thaumatin‐like protein (TLP 2) as an allergen in sapodilla plum (Manilkara zapota) |
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