Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis
•Seeds of pgp1pgp2 double mutants of Arabidopsis cannot germinate.•Embryos of pgp1pgp2 double mutants of Arabidopsis cannot synthesize PG.•PG is required for embryo development in Arabidopsis.•PG is required for normal membrane structure of chloroplasts and mitochondria. Phosphatidylglycerophosphate...
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| Veröffentlicht in: | FEBS letters 2014-05, Vol.588 (9), p.1680-1685 |
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| creator | Tanoue, Ryo Kobayashi, Megumi Katayama, Kenta Nagata, Noriko Wada, Hajime |
| description | •Seeds of pgp1pgp2 double mutants of Arabidopsis cannot germinate.•Embryos of pgp1pgp2 double mutants of Arabidopsis cannot synthesize PG.•PG is required for embryo development in Arabidopsis.•PG is required for normal membrane structure of chloroplasts and mitochondria.
Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the majority of seeds did not germinate. Thylakoid membranes did not develop in plastids, mitochondrial membrane structures were abnormal in the mutant embryos, and radiolabeling of phospholipids showed that radioactivity was not significantly incorporated into PG. These results demonstrated that PG biosynthesis is essential for the development of embryos and normal membrane structures of chloroplasts and mitochondria. |
| doi_str_mv | 10.1016/j.febslet.2014.03.010 |
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Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the majority of seeds did not germinate. Thylakoid membranes did not develop in plastids, mitochondrial membrane structures were abnormal in the mutant embryos, and radiolabeling of phospholipids showed that radioactivity was not significantly incorporated into PG. These results demonstrated that PG biosynthesis is essential for the development of embryos and normal membrane structures of chloroplasts and mitochondria.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.03.010</identifier><identifier>PMID: 24632290</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis - ultrastructure ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; cardiolipin ; Chloroplast ; Chloroplasts - metabolism ; Chloroplasts - ultrastructure ; Development ; DGDG ; digalactosyldiacylglycerol ; Embryo ; gas chromatography ; Germination ; MGDG ; Mitochondria - metabolism ; Mitochondria - ultrastructure ; Mitochondrial Membranes - metabolism ; Mitochondrial Membranes - ultrastructure ; Mitochondrion ; monogalactosyldiacylglycerol ; Murashige and Skoog ; PGP ; phosphatidic acid ; Phosphatidylglycerol ; Phosphatidylglycerols - biosynthesis ; phosphatidylglycerophosphate ; Phospholipid ; Phospholipids - metabolism ; Seeds - growth & development ; Seeds - metabolism ; Seeds - ultrastructure ; SQDG ; sulfoquinovosyldiacylglycerol ; thin-layer chromatography ; Thylakoids - metabolism ; Thylakoids - ultrastructure ; TLC</subject><ispartof>FEBS letters, 2014-05, Vol.588 (9), p.1680-1685</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543X-419423e9329e70db386ac59e97e557e9cf03b951c0370a2ceb503d0101e1143</citedby><cites>FETCH-LOGICAL-c543X-419423e9329e70db386ac59e97e557e9cf03b951c0370a2ceb503d0101e1143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.03.010$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S001457931400204X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24632290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanoue, Ryo</creatorcontrib><creatorcontrib>Kobayashi, Megumi</creatorcontrib><creatorcontrib>Katayama, Kenta</creatorcontrib><creatorcontrib>Nagata, Noriko</creatorcontrib><creatorcontrib>Wada, Hajime</creatorcontrib><title>Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Seeds of pgp1pgp2 double mutants of Arabidopsis cannot germinate.•Embryos of pgp1pgp2 double mutants of Arabidopsis cannot synthesize PG.•PG is required for embryo development in Arabidopsis.•PG is required for normal membrane structure of chloroplasts and mitochondria.
Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the majority of seeds did not germinate. Thylakoid membranes did not develop in plastids, mitochondrial membrane structures were abnormal in the mutant embryos, and radiolabeling of phospholipids showed that radioactivity was not significantly incorporated into PG. These results demonstrated that PG biosynthesis is essential for the development of embryos and normal membrane structures of chloroplasts and mitochondria.</description><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - ultrastructure</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>cardiolipin</subject><subject>Chloroplast</subject><subject>Chloroplasts - metabolism</subject><subject>Chloroplasts - ultrastructure</subject><subject>Development</subject><subject>DGDG</subject><subject>digalactosyldiacylglycerol</subject><subject>Embryo</subject><subject>gas chromatography</subject><subject>Germination</subject><subject>MGDG</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - ultrastructure</subject><subject>Mitochondrial Membranes - metabolism</subject><subject>Mitochondrial Membranes - ultrastructure</subject><subject>Mitochondrion</subject><subject>monogalactosyldiacylglycerol</subject><subject>Murashige and Skoog</subject><subject>PGP</subject><subject>phosphatidic acid</subject><subject>Phosphatidylglycerol</subject><subject>Phosphatidylglycerols - biosynthesis</subject><subject>phosphatidylglycerophosphate</subject><subject>Phospholipid</subject><subject>Phospholipids - metabolism</subject><subject>Seeds - growth & development</subject><subject>Seeds - metabolism</subject><subject>Seeds - ultrastructure</subject><subject>SQDG</subject><subject>sulfoquinovosyldiacylglycerol</subject><subject>thin-layer chromatography</subject><subject>Thylakoids - metabolism</subject><subject>Thylakoids - ultrastructure</subject><subject>TLC</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EosPAI4C8ZJPgn2QyXqFStRSpEkhl0Z3l2DfEIydObacoz8LL4igDW5AsWb4-95yr-yH0lpKSEnr4cCo7aKODVDJCq5LwklDyDO3oseEFrw7H52hH8k9RN4JfoFcxnkh-H6l4iS5YdeCMCbJDv771Pk69StYs7odbNATvcGt9XMbUQ7QR5xPgcbYBDO58wLmMDTyB89MAY8K-wzC0YfERq9Hg0YdBOTysNTUCjinMOs0B4qrUvfPBT07FtMkHm7zu_WiCVdiO-DKo1ho_5eTX6EWnXIQ353uP7m-uv1_dFndfP3-5urwrdF3xh6KiomIcBGcCGmJafjwoXQsQDdR1A0J3hLeipprwhiimoa0JN3lbFCit-B6931yn4B9niEkONmpwLg_v5yhpzQjPAfnsUb1JdfAxBujkFOygwiIpkSsVeZJnKnKlIgmXOSf3vTtHzO0A5m_XHwxZcLsJfloHy_-5ypvrT-x-RbwSphUhjFQP2erjZgV5Y08WgozawqjBZIA6SePtP6b9DcvwulA</recordid><startdate>20140502</startdate><enddate>20140502</enddate><creator>Tanoue, Ryo</creator><creator>Kobayashi, Megumi</creator><creator>Katayama, Kenta</creator><creator>Nagata, Noriko</creator><creator>Wada, Hajime</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140502</creationdate><title>Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis</title><author>Tanoue, Ryo ; Kobayashi, Megumi ; Katayama, Kenta ; Nagata, Noriko ; Wada, Hajime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543X-419423e9329e70db386ac59e97e557e9cf03b951c0370a2ceb503d0101e1143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - ultrastructure</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>cardiolipin</topic><topic>Chloroplast</topic><topic>Chloroplasts - metabolism</topic><topic>Chloroplasts - ultrastructure</topic><topic>Development</topic><topic>DGDG</topic><topic>digalactosyldiacylglycerol</topic><topic>Embryo</topic><topic>gas chromatography</topic><topic>Germination</topic><topic>MGDG</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - ultrastructure</topic><topic>Mitochondrial Membranes - metabolism</topic><topic>Mitochondrial Membranes - ultrastructure</topic><topic>Mitochondrion</topic><topic>monogalactosyldiacylglycerol</topic><topic>Murashige and Skoog</topic><topic>PGP</topic><topic>phosphatidic acid</topic><topic>Phosphatidylglycerol</topic><topic>Phosphatidylglycerols - biosynthesis</topic><topic>phosphatidylglycerophosphate</topic><topic>Phospholipid</topic><topic>Phospholipids - metabolism</topic><topic>Seeds - growth & development</topic><topic>Seeds - metabolism</topic><topic>Seeds - ultrastructure</topic><topic>SQDG</topic><topic>sulfoquinovosyldiacylglycerol</topic><topic>thin-layer chromatography</topic><topic>Thylakoids - metabolism</topic><topic>Thylakoids - ultrastructure</topic><topic>TLC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanoue, Ryo</creatorcontrib><creatorcontrib>Kobayashi, Megumi</creatorcontrib><creatorcontrib>Katayama, Kenta</creatorcontrib><creatorcontrib>Nagata, Noriko</creatorcontrib><creatorcontrib>Wada, Hajime</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanoue, Ryo</au><au>Kobayashi, Megumi</au><au>Katayama, Kenta</au><au>Nagata, Noriko</au><au>Wada, Hajime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-05-02</date><risdate>2014</risdate><volume>588</volume><issue>9</issue><spage>1680</spage><epage>1685</epage><pages>1680-1685</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Seeds of pgp1pgp2 double mutants of Arabidopsis cannot germinate.•Embryos of pgp1pgp2 double mutants of Arabidopsis cannot synthesize PG.•PG is required for embryo development in Arabidopsis.•PG is required for normal membrane structure of chloroplasts and mitochondria.
Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the majority of seeds did not germinate. Thylakoid membranes did not develop in plastids, mitochondrial membrane structures were abnormal in the mutant embryos, and radiolabeling of phospholipids showed that radioactivity was not significantly incorporated into PG. These results demonstrated that PG biosynthesis is essential for the development of embryos and normal membrane structures of chloroplasts and mitochondria.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>24632290</pmid><doi>10.1016/j.febslet.2014.03.010</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis - ultrastructure Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism cardiolipin Chloroplast Chloroplasts - metabolism Chloroplasts - ultrastructure Development DGDG digalactosyldiacylglycerol Embryo gas chromatography Germination MGDG Mitochondria - metabolism Mitochondria - ultrastructure Mitochondrial Membranes - metabolism Mitochondrial Membranes - ultrastructure Mitochondrion monogalactosyldiacylglycerol Murashige and Skoog PGP phosphatidic acid Phosphatidylglycerol Phosphatidylglycerols - biosynthesis phosphatidylglycerophosphate Phospholipid Phospholipids - metabolism Seeds - growth & development Seeds - metabolism Seeds - ultrastructure SQDG sulfoquinovosyldiacylglycerol thin-layer chromatography Thylakoids - metabolism Thylakoids - ultrastructure TLC |
| title | Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis |
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