Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells
Triptolide, a diterpene derived from Tripterygium wilfordii Hook f., a Chinese medicinal herb, has been reported to inhibit cell proliferation and induce apoptosis in various human cancer cells, but its anticancer effects on human osteosarcoma cells have not yet been elucidated. In this study, we in...
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| Veröffentlicht in: | Indian journal of biochemistry & biophysics 2013-12, Vol.50 (6), p.485-491 |
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| container_title | Indian journal of biochemistry & biophysics |
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| creator | Kwon, Haw-Young Kim, Kyoung-Sook An, Hyun-Kyu Moon, Hyung-In Kim, Hyun-Jun Lee, Young-Choon |
| description | Triptolide, a diterpene derived from Tripterygium wilfordii Hook f., a Chinese medicinal herb, has been reported to inhibit cell proliferation and induce apoptosis in various human cancer cells, but its anticancer effects on human osteosarcoma cells have not yet been elucidated. In this study, we investigated whether triptolide induces apoptosis in human osteosarcoma cells and the underlying molecular mechanisms. We firstly demonstrated that triptolide inhibited cell growth and induced apoptosis in U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2, Bid and mitochondrial cytochrome c were downregulated in triptolide-treated U2OS cells, whereas the levels of Fas, FasL, Bax, cytosolic cytochrome c, cleaved caspase-3 and cleaved PARP were upregulated. These results suggest that triptolide induces apoptosis in U2OS cells by activating both death receptor and mitochondrial apoptotic pathways. |
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In this study, we investigated whether triptolide induces apoptosis in human osteosarcoma cells and the underlying molecular mechanisms. We firstly demonstrated that triptolide inhibited cell growth and induced apoptosis in U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2, Bid and mitochondrial cytochrome c were downregulated in triptolide-treated U2OS cells, whereas the levels of Fas, FasL, Bax, cytosolic cytochrome c, cleaved caspase-3 and cleaved PARP were upregulated. 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In this study, we investigated whether triptolide induces apoptosis in human osteosarcoma cells and the underlying molecular mechanisms. We firstly demonstrated that triptolide inhibited cell growth and induced apoptosis in U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2, Bid and mitochondrial cytochrome c were downregulated in triptolide-treated U2OS cells, whereas the levels of Fas, FasL, Bax, cytosolic cytochrome c, cleaved caspase-3 and cleaved PARP were upregulated. These results suggest that triptolide induces apoptosis in U2OS cells by activating both death receptor and mitochondrial apoptotic pathways.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Diterpenes - pharmacology</subject><subject>Enzyme Activation - drug effects</subject><subject>Epoxy Compounds - pharmacology</subject><subject>G2 Phase Cell Cycle Checkpoints - drug effects</subject><subject>Humans</subject><subject>M Phase Cell Cycle Checkpoints - drug effects</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Osteosarcoma - pathology</subject><subject>Phenanthrenes - pharmacology</subject><subject>Poly(ADP-ribose) Polymerases - metabolism</subject><subject>Proteolysis - drug effects</subject><issn>0301-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kM1qwzAQhH1oadK0r1B07MWwkiwrOZbQPwjk0ORsNpJcq9iWq5Vp8_Z1SXIaZvgYhrnK5iCB51zAcpbdEn0BlKUS6iabiUJrsdJintW76IcUWm8d870djSOGQ5gi8sRSE8P42TD3m6LvyRuGvZ24ixswNT94pClizdhhzwIlFwijCR2yvdh-MOPalu6y6xpbcvdnXWT7l-fd-i3fbF_f10-bfBCcp1zLUh7QWqOQg8IaihVyC6YAw2u9rLVaWovTcEBQCrQwhayt5NZhaVALucgeT71DDN-jo1R1nv4XYO_CSBVXAjisRAkT-nBGx0PnbDVE32E8Vpdv5B_T9mEv</recordid><startdate>201312</startdate><enddate>201312</enddate><creator>Kwon, Haw-Young</creator><creator>Kim, Kyoung-Sook</creator><creator>An, Hyun-Kyu</creator><creator>Moon, Hyung-In</creator><creator>Kim, Hyun-Jun</creator><creator>Lee, Young-Choon</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201312</creationdate><title>Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells</title><author>Kwon, Haw-Young ; Kim, Kyoung-Sook ; An, Hyun-Kyu ; Moon, Hyung-In ; Kim, Hyun-Jun ; Lee, Young-Choon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-7363baddc5a105af049a1d0c40c1f78f758dda9720a055072c43fd31dea6ca723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Diterpenes - pharmacology</topic><topic>Enzyme Activation - drug effects</topic><topic>Epoxy Compounds - pharmacology</topic><topic>G2 Phase Cell Cycle Checkpoints - drug effects</topic><topic>Humans</topic><topic>M Phase Cell Cycle Checkpoints - drug effects</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Osteosarcoma - pathology</topic><topic>Phenanthrenes - pharmacology</topic><topic>Poly(ADP-ribose) Polymerases - metabolism</topic><topic>Proteolysis - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwon, Haw-Young</creatorcontrib><creatorcontrib>Kim, Kyoung-Sook</creatorcontrib><creatorcontrib>An, Hyun-Kyu</creatorcontrib><creatorcontrib>Moon, Hyung-In</creatorcontrib><creatorcontrib>Kim, Hyun-Jun</creatorcontrib><creatorcontrib>Lee, Young-Choon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Indian journal of biochemistry & biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwon, Haw-Young</au><au>Kim, Kyoung-Sook</au><au>An, Hyun-Kyu</au><au>Moon, Hyung-In</au><au>Kim, Hyun-Jun</au><au>Lee, Young-Choon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells</atitle><jtitle>Indian journal of biochemistry & biophysics</jtitle><addtitle>Indian J Biochem Biophys</addtitle><date>2013-12</date><risdate>2013</risdate><volume>50</volume><issue>6</issue><spage>485</spage><epage>491</epage><pages>485-491</pages><issn>0301-1208</issn><abstract>Triptolide, a diterpene derived from Tripterygium wilfordii Hook f., a Chinese medicinal herb, has been reported to inhibit cell proliferation and induce apoptosis in various human cancer cells, but its anticancer effects on human osteosarcoma cells have not yet been elucidated. In this study, we investigated whether triptolide induces apoptosis in human osteosarcoma cells and the underlying molecular mechanisms. We firstly demonstrated that triptolide inhibited cell growth and induced apoptosis in U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2, Bid and mitochondrial cytochrome c were downregulated in triptolide-treated U2OS cells, whereas the levels of Fas, FasL, Bax, cytosolic cytochrome c, cleaved caspase-3 and cleaved PARP were upregulated. These results suggest that triptolide induces apoptosis in U2OS cells by activating both death receptor and mitochondrial apoptotic pathways.</abstract><cop>India</cop><pmid>24772972</pmid><tpages>7</tpages></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Antineoplastic Agents - pharmacology Apoptosis - drug effects Caspase 3 - metabolism Cell Line, Tumor Cell Proliferation - drug effects Diterpenes - pharmacology Enzyme Activation - drug effects Epoxy Compounds - pharmacology G2 Phase Cell Cycle Checkpoints - drug effects Humans M Phase Cell Cycle Checkpoints - drug effects Mitochondria - drug effects Mitochondria - metabolism Osteosarcoma - pathology Phenanthrenes - pharmacology Poly(ADP-ribose) Polymerases - metabolism Proteolysis - drug effects |
| title | Triptolide induces apoptosis through extrinsic and intrinsic pathways in human osteosarcoma U2OS cells |
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