High-level coproduction, purification and characterisation of laccase and exopolysaccharides by Coriolus versicolor

•A two-stage pH-shift strategy was developed for coproduction of laccase and EPS.•Highest laccase and EPS production can reach 7680Ul−1 and 8.2gl−1, respectively.•Higher substrate conversion to metabolites rather than to biomass was obtained.•The MW and pI of laccase is 64kDa and 4.2, and it has α-h...

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Veröffentlicht in:	Food chemistry 2014-09, Vol.159, p.208-213
Hauptverfasser:	Que, Youxiong, Sun, Shujing, Xu, Liping, Zhang, Yuye, Zhu, Hu
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Coriolus versicolor Exopolysaccharides Fermentation Food toxicology Fungal Polysaccharides - biosynthesis Fungal Polysaccharides - isolation & purification Hydrogen-Ion Concentration Laccase Laccase - biosynthesis Laccase - isolation & purification Medical sciences Metabolite purification and accumulation pH controlled fermentation Toxicology Trametes - metabolism
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