Impaired motor coordination in Pitx3 overexpression mice
•We selectively overexpressed Pitx3 in intact midbrain DA neurons.•Pitx3 overexpressing mouse significantly affects the gene expression of midbrain dopamine neurons.•The expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo. Midbrain dopaminergic (DA) neuron...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2014-04, Vol.446 (4), p.1211-1218 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kim, Hongwon Quan, Xiaoyuan Seong, Yeju Kim, Jongpil |
| description | •We selectively overexpressed Pitx3 in intact midbrain DA neurons.•Pitx3 overexpressing mouse significantly affects the gene expression of midbrain dopamine neurons.•The expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo.
Midbrain dopaminergic (DA) neurons are involved in the regulation of voluntary movement and in emotion-related behaviors and are affected in Parkinson’s disease (PD). The homeodomain transcription factor Pitx3, which is uniquely expressed in midbrain DA neurons, plays a critical role in the development, function and maintenance of midbrain DA neurons. Pitx3 deficiency results in selective deficits of midbrain DA neurons in the substantia nigra pars compacta (SNc), reminiscent of the specific DA neuronal loss observed in PD. In this study, we found that selective overexpression of Pitx3 in intact midbrain DA neurons significantly affects the function of midbrain DA neurons. We observed changes in DA levels and gene expressions in mice overexpressing Pitx3. Furthermore, motor coordination and locomotion activities are significantly affected in mice overexpressing Pitx3, suggesting that the expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo. |
| doi_str_mv | 10.1016/j.bbrc.2014.03.085 |
| format | Article |
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Midbrain dopaminergic (DA) neurons are involved in the regulation of voluntary movement and in emotion-related behaviors and are affected in Parkinson’s disease (PD). The homeodomain transcription factor Pitx3, which is uniquely expressed in midbrain DA neurons, plays a critical role in the development, function and maintenance of midbrain DA neurons. Pitx3 deficiency results in selective deficits of midbrain DA neurons in the substantia nigra pars compacta (SNc), reminiscent of the specific DA neuronal loss observed in PD. In this study, we found that selective overexpression of Pitx3 in intact midbrain DA neurons significantly affects the function of midbrain DA neurons. We observed changes in DA levels and gene expressions in mice overexpressing Pitx3. Furthermore, motor coordination and locomotion activities are significantly affected in mice overexpressing Pitx3, suggesting that the expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.03.085</identifier><identifier>PMID: 24680684</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Dopamine - metabolism ; Dopaminergic Neurons - metabolism ; Dopaminergic Neurons - physiology ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Locomotion ; Male ; Mesencephalon - metabolism ; Mesencephalon - physiology ; Mice ; Mice, Inbred C57BL ; Midbrain DA neurons ; Models, Molecular ; Parkinson Disease - genetics ; Parkinson Disease - physiopathology ; Parkinson’s disease ; Pitx3 ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2014-04, Vol.446 (4), p.1211-1218</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-7950063e9453901c2e6136e945cbd036cd2ff7709b9664af267a6a0a7f8dbe793</citedby><cites>FETCH-LOGICAL-c356t-7950063e9453901c2e6136e945cbd036cd2ff7709b9664af267a6a0a7f8dbe793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X14005348$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24680684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hongwon</creatorcontrib><creatorcontrib>Quan, Xiaoyuan</creatorcontrib><creatorcontrib>Seong, Yeju</creatorcontrib><creatorcontrib>Kim, Jongpil</creatorcontrib><title>Impaired motor coordination in Pitx3 overexpression mice</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•We selectively overexpressed Pitx3 in intact midbrain DA neurons.•Pitx3 overexpressing mouse significantly affects the gene expression of midbrain dopamine neurons.•The expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo.
Midbrain dopaminergic (DA) neurons are involved in the regulation of voluntary movement and in emotion-related behaviors and are affected in Parkinson’s disease (PD). The homeodomain transcription factor Pitx3, which is uniquely expressed in midbrain DA neurons, plays a critical role in the development, function and maintenance of midbrain DA neurons. Pitx3 deficiency results in selective deficits of midbrain DA neurons in the substantia nigra pars compacta (SNc), reminiscent of the specific DA neuronal loss observed in PD. In this study, we found that selective overexpression of Pitx3 in intact midbrain DA neurons significantly affects the function of midbrain DA neurons. We observed changes in DA levels and gene expressions in mice overexpressing Pitx3. Furthermore, motor coordination and locomotion activities are significantly affected in mice overexpressing Pitx3, suggesting that the expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo.</description><subject>Animals</subject><subject>Dopamine - metabolism</subject><subject>Dopaminergic Neurons - metabolism</subject><subject>Dopaminergic Neurons - physiology</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Locomotion</subject><subject>Male</subject><subject>Mesencephalon - metabolism</subject><subject>Mesencephalon - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Midbrain DA neurons</subject><subject>Models, Molecular</subject><subject>Parkinson Disease - genetics</subject><subject>Parkinson Disease - physiopathology</subject><subject>Parkinson’s disease</subject><subject>Pitx3</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6BzxIj15aJ0mbNuBFFj8WFvSg4C2kyRSybJs16S7rv7dlV4-ehhmeeZl5CLmmkFGg4m6V1XUwGQOaZ8AzqIoTMqUgIWUU8lMyBQCRMkk_J-QixhUApbmQ52TCclGBqPIpqRbtRruANml970NivA_Wdbp3vktcl7y5fs8Tv8OA-03AGMd56wxekrNGryNeHeuMfDw9vs9f0uXr82L-sEwNL0SflrIYjuAo84JLoIahoFyMraktcGEsa5qyBFlLIXLdMFFqoUGXTWVrLCWfkdtD7ib4ry3GXrUuGlyvdYd-GxUtqGSC0qoaUHZATfAxBmzUJrhWh29FQY3G1EqNxtRoTAFXg7Fh6eaYv61btH8rv4oG4P4A4PDlzmFQ0TjsDNpBm-mV9e6__B9RKHuP</recordid><startdate>20140418</startdate><enddate>20140418</enddate><creator>Kim, Hongwon</creator><creator>Quan, Xiaoyuan</creator><creator>Seong, Yeju</creator><creator>Kim, Jongpil</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140418</creationdate><title>Impaired motor coordination in Pitx3 overexpression mice</title><author>Kim, Hongwon ; Quan, Xiaoyuan ; Seong, Yeju ; Kim, Jongpil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-7950063e9453901c2e6136e945cbd036cd2ff7709b9664af267a6a0a7f8dbe793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Dopamine - metabolism</topic><topic>Dopaminergic Neurons - metabolism</topic><topic>Dopaminergic Neurons - physiology</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Locomotion</topic><topic>Male</topic><topic>Mesencephalon - metabolism</topic><topic>Mesencephalon - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Midbrain DA neurons</topic><topic>Models, Molecular</topic><topic>Parkinson Disease - genetics</topic><topic>Parkinson Disease - physiopathology</topic><topic>Parkinson’s disease</topic><topic>Pitx3</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hongwon</creatorcontrib><creatorcontrib>Quan, Xiaoyuan</creatorcontrib><creatorcontrib>Seong, Yeju</creatorcontrib><creatorcontrib>Kim, Jongpil</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hongwon</au><au>Quan, Xiaoyuan</au><au>Seong, Yeju</au><au>Kim, Jongpil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impaired motor coordination in Pitx3 overexpression mice</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2014-04-18</date><risdate>2014</risdate><volume>446</volume><issue>4</issue><spage>1211</spage><epage>1218</epage><pages>1211-1218</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•We selectively overexpressed Pitx3 in intact midbrain DA neurons.•Pitx3 overexpressing mouse significantly affects the gene expression of midbrain dopamine neurons.•The expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo.
Midbrain dopaminergic (DA) neurons are involved in the regulation of voluntary movement and in emotion-related behaviors and are affected in Parkinson’s disease (PD). The homeodomain transcription factor Pitx3, which is uniquely expressed in midbrain DA neurons, plays a critical role in the development, function and maintenance of midbrain DA neurons. Pitx3 deficiency results in selective deficits of midbrain DA neurons in the substantia nigra pars compacta (SNc), reminiscent of the specific DA neuronal loss observed in PD. In this study, we found that selective overexpression of Pitx3 in intact midbrain DA neurons significantly affects the function of midbrain DA neurons. We observed changes in DA levels and gene expressions in mice overexpressing Pitx3. Furthermore, motor coordination and locomotion activities are significantly affected in mice overexpressing Pitx3, suggesting that the expression level of Pitx3 plays an important role in the function of midbrain DA neuron in vivo.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24680684</pmid><doi>10.1016/j.bbrc.2014.03.085</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Dopamine - metabolism Dopaminergic Neurons - metabolism Dopaminergic Neurons - physiology Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Locomotion Male Mesencephalon - metabolism Mesencephalon - physiology Mice Mice, Inbred C57BL Midbrain DA neurons Models, Molecular Parkinson Disease - genetics Parkinson Disease - physiopathology Parkinson’s disease Pitx3 Transcription Factors - genetics Transcription Factors - metabolism Up-Regulation |
| title | Impaired motor coordination in Pitx3 overexpression mice |
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