Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2

To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses (1.2, 3.8 and 12 mg x kg(-1)) of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffein...

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Veröffentlicht in:Yao hsüeh hsüeh pao 2014-02, Vol.49 (2), p.267-272
Hauptverfasser: Li, Xiang-Yang, Liu, Yong-Nian, Li, Yong-Ping, Zhu, Jun-Bo, Yao, Xing-Chen, Li, Yong-Fang, Yang, Mei, Yuan, Ming, Fan, Xue-Ru, Yin, Yue-Miao
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container_issue 2
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container_title Yao hsüeh hsüeh pao
container_volume 49
creator Li, Xiang-Yang
Liu, Yong-Nian
Li, Yong-Ping
Zhu, Jun-Bo
Yao, Xing-Chen
Li, Yong-Fang
Yang, Mei
Yuan, Ming
Fan, Xue-Ru
Yin, Yue-Miao
description To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses (1.2, 3.8 and 12 mg x kg(-1)) of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. After single administration of Zuotai 3.8 mg x kg(-1) and repeated administration of Zuotai 3.8 and 12 mg x kg(-1), the activity of CYP1A2 and NAT2 decreased significantly compared w
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Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. 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Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. 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subjects Administration, Oral
Animals
Arylamine N-Acetyltransferase - genetics
Arylamine N-Acetyltransferase - metabolism
Caffeine - metabolism
Caffeine - urine
Cytochrome P-450 CYP1A2 - genetics
Cytochrome P-450 CYP1A2 - metabolism
Dose-Response Relationship, Drug
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - pharmacology
Female
Male
Medicine, Tibetan Traditional
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Theophylline - urine
Uracil - analogs & derivatives
Uracil - urine
Uric Acid - analogs & derivatives
Uric Acid - urine
Xanthines - urine
title Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2
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