Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2
To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses (1.2, 3.8 and 12 mg x kg(-1)) of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffein...
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Veröffentlicht in: | Yao hsüeh hsüeh pao 2014-02, Vol.49 (2), p.267-272 |
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creator | Li, Xiang-Yang Liu, Yong-Nian Li, Yong-Ping Zhu, Jun-Bo Yao, Xing-Chen Li, Yong-Fang Yang, Mei Yuan, Ming Fan, Xue-Ru Yin, Yue-Miao |
description | To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses (1.2, 3.8 and 12 mg x kg(-1)) of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. After single administration of Zuotai 3.8 mg x kg(-1) and repeated administration of Zuotai 3.8 and 12 mg x kg(-1), the activity of CYP1A2 and NAT2 decreased significantly compared w |
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Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. After single administration of Zuotai 3.8 mg x kg(-1) and repeated administration of Zuotai 3.8 and 12 mg x kg(-1), the activity of CYP1A2 and NAT2 decreased significantly compared w</description><identifier>ISSN: 0513-4870</identifier><identifier>PMID: 24761621</identifier><language>chi</language><publisher>China</publisher><subject>Administration, Oral ; Animals ; Arylamine N-Acetyltransferase - genetics ; Arylamine N-Acetyltransferase - metabolism ; Caffeine - metabolism ; Caffeine - urine ; Cytochrome P-450 CYP1A2 - genetics ; Cytochrome P-450 CYP1A2 - metabolism ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal - administration & dosage ; Drugs, Chinese Herbal - pharmacology ; Female ; Male ; Medicine, Tibetan Traditional ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - metabolism ; Theophylline - urine ; Uracil - analogs & derivatives ; Uracil - urine ; Uric Acid - analogs & derivatives ; Uric Acid - urine ; Xanthines - urine</subject><ispartof>Yao hsüeh hsüeh pao, 2014-02, Vol.49 (2), p.267-272</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24761621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiang-Yang</creatorcontrib><creatorcontrib>Liu, Yong-Nian</creatorcontrib><creatorcontrib>Li, Yong-Ping</creatorcontrib><creatorcontrib>Zhu, Jun-Bo</creatorcontrib><creatorcontrib>Yao, Xing-Chen</creatorcontrib><creatorcontrib>Li, Yong-Fang</creatorcontrib><creatorcontrib>Yang, Mei</creatorcontrib><creatorcontrib>Yuan, Ming</creatorcontrib><creatorcontrib>Fan, Xue-Ru</creatorcontrib><creatorcontrib>Yin, Yue-Miao</creatorcontrib><title>Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2</title><title>Yao hsüeh hsüeh pao</title><addtitle>Yao Xue Xue Bao</addtitle><description>To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses (1.2, 3.8 and 12 mg x kg(-1)) of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. After single administration of Zuotai 3.8 mg x kg(-1) and repeated administration of Zuotai 3.8 and 12 mg x kg(-1), the activity of CYP1A2 and NAT2 decreased significantly compared w</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Arylamine N-Acetyltransferase - genetics</subject><subject>Arylamine N-Acetyltransferase - metabolism</subject><subject>Caffeine - metabolism</subject><subject>Caffeine - urine</subject><subject>Cytochrome P-450 CYP1A2 - genetics</subject><subject>Cytochrome P-450 CYP1A2 - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drugs, Chinese Herbal - administration & dosage</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Female</subject><subject>Male</subject><subject>Medicine, Tibetan Traditional</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - metabolism</subject><subject>Theophylline - urine</subject><subject>Uracil - analogs & derivatives</subject><subject>Uracil - urine</subject><subject>Uric Acid - analogs & derivatives</subject><subject>Uric Acid - urine</subject><subject>Xanthines - urine</subject><issn>0513-4870</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kD1PwzAYhD2AaCn8BeSRgUj-iu2OUVU-pKogFAamyHFeC6PECbGDKL-eCMp0wz063d0JWpKc8kxoRRboPMZ3QgRdc32GFkwoSSWjS2S2zoFNuHe49DUkE3AHjbc-AP6e-mQ87gNOb4CNTf7Tp8MNHsY-gQ_YhAZ3z_sCw9cwQox-JueczesTLdivuy9KdoFOnWkjXB51hV5ut-XmPts93j1sil02UCZTpijhXFOWWyAWmHWSM9Iwx5WrRWMVSMl1LoSVWhDS0Fxpzp3RjOha25rxFbr-y53rfUwQU9X5aKFtTYB-ihXN6Zrlkksyo1dHdKrntdUw-s6Mh-r_Fv4DX-lbCA</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Li, Xiang-Yang</creator><creator>Liu, Yong-Nian</creator><creator>Li, Yong-Ping</creator><creator>Zhu, Jun-Bo</creator><creator>Yao, Xing-Chen</creator><creator>Li, Yong-Fang</creator><creator>Yang, Mei</creator><creator>Yuan, Ming</creator><creator>Fan, Xue-Ru</creator><creator>Yin, Yue-Miao</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201402</creationdate><title>Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2</title><author>Li, Xiang-Yang ; Liu, Yong-Nian ; Li, Yong-Ping ; Zhu, Jun-Bo ; Yao, Xing-Chen ; Li, Yong-Fang ; Yang, Mei ; Yuan, Ming ; Fan, Xue-Ru ; Yin, Yue-Miao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-710338125ce0ce2cf6320d2f37fb4dc7e6638544c68400d157833fa8208b8cb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2014</creationdate><topic>Administration, Oral</topic><topic>Animals</topic><topic>Arylamine N-Acetyltransferase - genetics</topic><topic>Arylamine N-Acetyltransferase - metabolism</topic><topic>Caffeine - metabolism</topic><topic>Caffeine - urine</topic><topic>Cytochrome P-450 CYP1A2 - genetics</topic><topic>Cytochrome P-450 CYP1A2 - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drugs, Chinese Herbal - administration & dosage</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Female</topic><topic>Male</topic><topic>Medicine, Tibetan Traditional</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - metabolism</topic><topic>Theophylline - urine</topic><topic>Uracil - analogs & derivatives</topic><topic>Uracil - urine</topic><topic>Uric Acid - analogs & derivatives</topic><topic>Uric Acid - urine</topic><topic>Xanthines - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiang-Yang</creatorcontrib><creatorcontrib>Liu, Yong-Nian</creatorcontrib><creatorcontrib>Li, Yong-Ping</creatorcontrib><creatorcontrib>Zhu, Jun-Bo</creatorcontrib><creatorcontrib>Yao, Xing-Chen</creatorcontrib><creatorcontrib>Li, Yong-Fang</creatorcontrib><creatorcontrib>Yang, Mei</creatorcontrib><creatorcontrib>Yuan, Ming</creatorcontrib><creatorcontrib>Fan, Xue-Ru</creatorcontrib><creatorcontrib>Yin, Yue-Miao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Yao hsüeh hsüeh pao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiang-Yang</au><au>Liu, Yong-Nian</au><au>Li, Yong-Ping</au><au>Zhu, Jun-Bo</au><au>Yao, Xing-Chen</au><au>Li, Yong-Fang</au><au>Yang, Mei</au><au>Yuan, Ming</au><au>Fan, Xue-Ru</au><au>Yin, Yue-Miao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2</atitle><jtitle>Yao hsüeh hsüeh pao</jtitle><addtitle>Yao Xue Xue Bao</addtitle><date>2014-02</date><risdate>2014</risdate><volume>49</volume><issue>2</issue><spage>267</spage><epage>272</epage><pages>267-272</pages><issn>0513-4870</issn><abstract>To study the effect of Tibetan medicine Zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2, three different doses (1.2, 3.8 and 12 mg x kg(-1)) of Zuotai were administrated orally to rats once a day or once daily for twelve days, separately. Rats were administrated orally caffeine (CF) on the second day after Zuotai administration, and the urine concentration of CF metabolite 5-acetylamino-6-formylamino-3-methyl-uracil (AFMU), 1-methyluric acid (1U), 1-methylxanthine (1X), 1, 7-dimethylxanthine (17U) at 5 h after study drug administration was determined by RP-HPLC. The activity of CYP1A2 and NAT2 was evaluated by the ratio of metabolites (AFMU+1X+1U)/17U and the ratio of AFMU/(AFMU+1X+1U), respectively. The protein and mRNA expression of CYP1A2 and NAT2 were determined by ELISA and RT-PCR method, respectively. After single administration of Zuotai 3.8 mg x kg(-1) and repeated administration of Zuotai 3.8 and 12 mg x kg(-1), the activity of CYP1A2 and NAT2 decreased significantly compared w</abstract><cop>China</cop><pmid>24761621</pmid><tpages>6</tpages></addata></record> |
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subjects | Administration, Oral Animals Arylamine N-Acetyltransferase - genetics Arylamine N-Acetyltransferase - metabolism Caffeine - metabolism Caffeine - urine Cytochrome P-450 CYP1A2 - genetics Cytochrome P-450 CYP1A2 - metabolism Dose-Response Relationship, Drug Drugs, Chinese Herbal - administration & dosage Drugs, Chinese Herbal - pharmacology Female Male Medicine, Tibetan Traditional Rats Rats, Sprague-Dawley RNA, Messenger - metabolism Theophylline - urine Uracil - analogs & derivatives Uracil - urine Uric Acid - analogs & derivatives Uric Acid - urine Xanthines - urine |
title | Effect of Tibetan medicine zuotai on the activity, protein and mRNA expression of CYP1A2 and NAT2 |
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