A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis
Although Campylobacter jejuni and Campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other Campylobacter species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin (cdt) gene-based PCR-RFLP assay for th...
Gespeichert in:
| Veröffentlicht in: | Journal of medical microbiology 2014-05, Vol.63 (5), p.659-666 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 666 |
|---|---|
| container_issue | 5 |
| container_start_page | 659 |
| container_title | Journal of medical microbiology |
| container_volume | 63 |
| creator | KAMEI, Kazumasa ASAKURA, Masahiro SOMROOP, Srinuan HATANAKA, Noritoshi HINENOYA, Atsushi NAGITA, Akira MISAWA, Naoaki MATSUDA, Motoo NAKAGAWA, Shinsaku YAMASAKI, Shinji |
| description | Although Campylobacter jejuni and Campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other Campylobacter species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin (cdt) gene-based PCR-RFLP assay for the detection and differentiation of C. jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis. Previously designed common primers, which can amplify the cdtB gene of C. jejuni, C. coli and C. fetus, were used for detecting seven Campylobacter species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 C. jejuni, 19 C. coli, 20 C. fetus, 24 C. hyointestinalis, 13 C. lari, 2 C. helveticus, 22 C. upsaliensis, 3 other Campylobacter spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for C. hyointestinalis (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated C. jejuni, C. coli and C. fetus in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven Campylobacter species important for human and animal diseases. |
| doi_str_mv | 10.1099/jmm.0.071498-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1518814336</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1518814336</sourcerecordid><originalsourceid>FETCH-LOGICAL-c431t-aceda1e1899f215a199f7ceafbd4db269346c2466e52837af87423d9226655cc3</originalsourceid><addsrcrecordid>eNpFkU-L1EAQxRtR3HH16lH6IuzBZPtfOp3jElwVBlyW9RxqOtVMD51k7O4I8238qGYno57q8fjVq4JHyHvOSs6a5vYwDCUrWc1VYwr2gmy4qmVRaaVekg1jQhRC8-qKvEnpwBivpWxekyuhKm2MERvy-44-tI_F4_32gUJKcKJuijTvkfaY0WY_jRTGnvbeOYw4Zg9nb3K0heF4CtMObMZID3iYR_-JtiW1U1iFwzyns9qfJj9mTNmPEPzqBYgrtsfwC7O3czqfWpz5mBYMx-TTW_LKQUj47jKvyY_7z0_t12L7_cu39m5bWCV5LsBiDxy5aRoneAV8mbVFcLte9TuhG6m0FUprrISRNThTKyH7Rgitq8paeU1u1txjnH7Oy6fd4JPFEGDEaU4dr7gxXEmpF7RcURunlCK67hj9APHUcdY9t9ItrXSsW1vp2LLw4ZI97wbs_-F_a1iAjxcAkoXgIozWp_-cUbphTMs_MWyUbA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1518814336</pqid></control><display><type>article</type><title>A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>KAMEI, Kazumasa ; ASAKURA, Masahiro ; SOMROOP, Srinuan ; HATANAKA, Noritoshi ; HINENOYA, Atsushi ; NAGITA, Akira ; MISAWA, Naoaki ; MATSUDA, Motoo ; NAKAGAWA, Shinsaku ; YAMASAKI, Shinji</creator><creatorcontrib>KAMEI, Kazumasa ; ASAKURA, Masahiro ; SOMROOP, Srinuan ; HATANAKA, Noritoshi ; HINENOYA, Atsushi ; NAGITA, Akira ; MISAWA, Naoaki ; MATSUDA, Motoo ; NAKAGAWA, Shinsaku ; YAMASAKI, Shinji</creatorcontrib><description>Although Campylobacter jejuni and Campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other Campylobacter species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin (cdt) gene-based PCR-RFLP assay for the detection and differentiation of C. jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis. Previously designed common primers, which can amplify the cdtB gene of C. jejuni, C. coli and C. fetus, were used for detecting seven Campylobacter species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 C. jejuni, 19 C. coli, 20 C. fetus, 24 C. hyointestinalis, 13 C. lari, 2 C. helveticus, 22 C. upsaliensis, 3 other Campylobacter spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for C. hyointestinalis (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated C. jejuni, C. coli and C. fetus in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven Campylobacter species important for human and animal diseases.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/jmm.0.071498-0</identifier><identifier>PMID: 24568882</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Adolescent ; Animals ; Bacterial Toxins - genetics ; Bacteriology ; Biological and medical sciences ; Campylobacter - classification ; Campylobacter - isolation & purification ; Campylobacter Infections - diagnosis ; Campylobacter Infections - microbiology ; Cattle ; Child ; Child, Preschool ; DNA Primers - genetics ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Infant ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Diagnostic Techniques - methods ; Molecular Sequence Data ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Sensitivity and Specificity ; Sequence Analysis, DNA</subject><ispartof>Journal of medical microbiology, 2014-05, Vol.63 (5), p.659-666</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-aceda1e1899f215a199f7ceafbd4db269346c2466e52837af87423d9226655cc3</citedby><cites>FETCH-LOGICAL-c431t-aceda1e1899f215a199f7ceafbd4db269346c2466e52837af87423d9226655cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3744,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28469006$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24568882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KAMEI, Kazumasa</creatorcontrib><creatorcontrib>ASAKURA, Masahiro</creatorcontrib><creatorcontrib>SOMROOP, Srinuan</creatorcontrib><creatorcontrib>HATANAKA, Noritoshi</creatorcontrib><creatorcontrib>HINENOYA, Atsushi</creatorcontrib><creatorcontrib>NAGITA, Akira</creatorcontrib><creatorcontrib>MISAWA, Naoaki</creatorcontrib><creatorcontrib>MATSUDA, Motoo</creatorcontrib><creatorcontrib>NAKAGAWA, Shinsaku</creatorcontrib><creatorcontrib>YAMASAKI, Shinji</creatorcontrib><title>A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>Although Campylobacter jejuni and Campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other Campylobacter species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin (cdt) gene-based PCR-RFLP assay for the detection and differentiation of C. jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis. Previously designed common primers, which can amplify the cdtB gene of C. jejuni, C. coli and C. fetus, were used for detecting seven Campylobacter species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 C. jejuni, 19 C. coli, 20 C. fetus, 24 C. hyointestinalis, 13 C. lari, 2 C. helveticus, 22 C. upsaliensis, 3 other Campylobacter spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for C. hyointestinalis (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated C. jejuni, C. coli and C. fetus in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven Campylobacter species important for human and animal diseases.</description><subject>Adolescent</subject><subject>Animals</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Campylobacter - classification</subject><subject>Campylobacter - isolation & purification</subject><subject>Campylobacter Infections - diagnosis</subject><subject>Campylobacter Infections - microbiology</subject><subject>Cattle</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>DNA Primers - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infant</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Analysis, DNA</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU-L1EAQxRtR3HH16lH6IuzBZPtfOp3jElwVBlyW9RxqOtVMD51k7O4I8238qGYno57q8fjVq4JHyHvOSs6a5vYwDCUrWc1VYwr2gmy4qmVRaaVekg1jQhRC8-qKvEnpwBivpWxekyuhKm2MERvy-44-tI_F4_32gUJKcKJuijTvkfaY0WY_jRTGnvbeOYw4Zg9nb3K0heF4CtMObMZID3iYR_-JtiW1U1iFwzyns9qfJj9mTNmPEPzqBYgrtsfwC7O3czqfWpz5mBYMx-TTW_LKQUj47jKvyY_7z0_t12L7_cu39m5bWCV5LsBiDxy5aRoneAV8mbVFcLte9TuhG6m0FUprrISRNThTKyH7Rgitq8paeU1u1txjnH7Oy6fd4JPFEGDEaU4dr7gxXEmpF7RcURunlCK67hj9APHUcdY9t9ItrXSsW1vp2LLw4ZI97wbs_-F_a1iAjxcAkoXgIozWp_-cUbphTMs_MWyUbA</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>KAMEI, Kazumasa</creator><creator>ASAKURA, Masahiro</creator><creator>SOMROOP, Srinuan</creator><creator>HATANAKA, Noritoshi</creator><creator>HINENOYA, Atsushi</creator><creator>NAGITA, Akira</creator><creator>MISAWA, Naoaki</creator><creator>MATSUDA, Motoo</creator><creator>NAKAGAWA, Shinsaku</creator><creator>YAMASAKI, Shinji</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140501</creationdate><title>A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis</title><author>KAMEI, Kazumasa ; ASAKURA, Masahiro ; SOMROOP, Srinuan ; HATANAKA, Noritoshi ; HINENOYA, Atsushi ; NAGITA, Akira ; MISAWA, Naoaki ; MATSUDA, Motoo ; NAKAGAWA, Shinsaku ; YAMASAKI, Shinji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-aceda1e1899f215a199f7ceafbd4db269346c2466e52837af87423d9226655cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adolescent</topic><topic>Animals</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Campylobacter - classification</topic><topic>Campylobacter - isolation & purification</topic><topic>Campylobacter Infections - diagnosis</topic><topic>Campylobacter Infections - microbiology</topic><topic>Cattle</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>DNA Primers - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KAMEI, Kazumasa</creatorcontrib><creatorcontrib>ASAKURA, Masahiro</creatorcontrib><creatorcontrib>SOMROOP, Srinuan</creatorcontrib><creatorcontrib>HATANAKA, Noritoshi</creatorcontrib><creatorcontrib>HINENOYA, Atsushi</creatorcontrib><creatorcontrib>NAGITA, Akira</creatorcontrib><creatorcontrib>MISAWA, Naoaki</creatorcontrib><creatorcontrib>MATSUDA, Motoo</creatorcontrib><creatorcontrib>NAKAGAWA, Shinsaku</creatorcontrib><creatorcontrib>YAMASAKI, Shinji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KAMEI, Kazumasa</au><au>ASAKURA, Masahiro</au><au>SOMROOP, Srinuan</au><au>HATANAKA, Noritoshi</au><au>HINENOYA, Atsushi</au><au>NAGITA, Akira</au><au>MISAWA, Naoaki</au><au>MATSUDA, Motoo</au><au>NAKAGAWA, Shinsaku</au><au>YAMASAKI, Shinji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>63</volume><issue>5</issue><spage>659</spage><epage>666</epage><pages>659-666</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>Although Campylobacter jejuni and Campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other Campylobacter species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin (cdt) gene-based PCR-RFLP assay for the detection and differentiation of C. jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis. Previously designed common primers, which can amplify the cdtB gene of C. jejuni, C. coli and C. fetus, were used for detecting seven Campylobacter species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 C. jejuni, 19 C. coli, 20 C. fetus, 24 C. hyointestinalis, 13 C. lari, 2 C. helveticus, 22 C. upsaliensis, 3 other Campylobacter spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for C. hyointestinalis (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated C. jejuni, C. coli and C. fetus in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven Campylobacter species important for human and animal diseases.</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>24568882</pmid><doi>10.1099/jmm.0.071498-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2615 |
| ispartof | Journal of medical microbiology, 2014-05, Vol.63 (5), p.659-666 |
| issn | 0022-2615 1473-5644 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1518814336 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adolescent Animals Bacterial Toxins - genetics Bacteriology Biological and medical sciences Campylobacter - classification Campylobacter - isolation & purification Campylobacter Infections - diagnosis Campylobacter Infections - microbiology Cattle Child Child, Preschool DNA Primers - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Female Fundamental and applied biological sciences. Psychology Humans Infant Infectious diseases Male Medical sciences Microbiology Miscellaneous Molecular Diagnostic Techniques - methods Molecular Sequence Data Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Sensitivity and Specificity Sequence Analysis, DNA |
| title | A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T21%3A05%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20PCR-RFLP%20assay%20for%20the%20detection%20and%20differentiation%20of%20Campylobacter%20jejuni,%20C.%20coli,%20C.%20fetus,%20C.%20hyointestinalis,%20C.%20lari,%20C.%20helveticus%20and%20C.%20upsaliensis&rft.jtitle=Journal%20of%20medical%20microbiology&rft.au=KAMEI,%20Kazumasa&rft.date=2014-05-01&rft.volume=63&rft.issue=5&rft.spage=659&rft.epage=666&rft.pages=659-666&rft.issn=0022-2615&rft.eissn=1473-5644&rft.coden=JMMIAV&rft_id=info:doi/10.1099/jmm.0.071498-0&rft_dat=%3Cproquest_cross%3E1518814336%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1518814336&rft_id=info:pmid/24568882&rfr_iscdi=true |