Influence of age on leptin induced skeletal muscle signalling
Aim Age associated fat mass accumulation could be because of dysregulation of leptin signalling in skeletal muscle. Thus, we investigated total protein expression and phosphorylation levels of the long isoform of the leptin receptor (OB‐Rb), and leptin signalling through janus kinase 2 (JAK2)/signal...
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| Veröffentlicht in: | Acta Physiologica 2014-05, Vol.211 (1), p.214-228 |
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| creator | Guadalupe-Grau, A. Larsen, S. Guerra, B. Calbet, J. A. L. Dela, F. Helge, J. W. |
| description | Aim
Age associated fat mass accumulation could be because of dysregulation of leptin signalling in skeletal muscle. Thus, we investigated total protein expression and phosphorylation levels of the long isoform of the leptin receptor (OB‐Rb), and leptin signalling through janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3), insulin receptor substrate 1 (IRS‐1), AMP‐activated protein kinase (AMPK) and acetyl‐coenzyme A carboxylase (ACC), combined with the leptin signalling inhibitors suppressor of cytokine signalling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) in human skeletal muscle of different age.
Methods
Vastus lateralis muscle biopsies were obtained from 39 men matched for BMI |
| doi_str_mv | 10.1111/apha.12273 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1518621607</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1518621607</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4313-a59dd28d6e7b063cd2dc9430bcd14bd8118d2e35ad0bba9aca9d05a69c5171793</originalsourceid><addsrcrecordid>eNp90EFLwzAUB_Agio7pxQ8gBS8idOYlbdIePIyhUxnqRBG8hDR5m3VZO5sV3be3s7qDB3N5Ofzen-RPyCHQHjTnTC9edQ8Yk3yLdEBGSQgSxPbmTpM9cuD9G6UUGPCIsV2yxyJB45SJDjm_LiauxsJgUE4CPW1GEThcLPMiyAtbG7SBn6HDpXbBvPbGYeDzaaGdy4vpPtmZaOfx4Gd2ydPlxePgKhzdDa8H_VFoIg481HFqLUusQJlRwY1l1qQRp5mxEGU2AUgsQx5rS7NMp9ro1NJYi9TEzWdkyrvkpM1dVOV7jX6p5rk36JwusKy9ghgSwUBQ2dDjP_StrKvmvd9KxpHkgjbqtFWmKr2vcKIWVT7X1UoBVete1bpX9d1rg49-IutsjnZDf1tsALTgI3e4-idK9e-v-r-hYbuT-yV-bnZ0NVNCchmr59uheknl5c1w_KDG_As57ZAb</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1517547360</pqid></control><display><type>article</type><title>Influence of age on leptin induced skeletal muscle signalling</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Guadalupe-Grau, A. ; Larsen, S. ; Guerra, B. ; Calbet, J. A. L. ; Dela, F. ; Helge, J. W.</creator><creatorcontrib>Guadalupe-Grau, A. ; Larsen, S. ; Guerra, B. ; Calbet, J. A. L. ; Dela, F. ; Helge, J. W.</creatorcontrib><description>Aim
Age associated fat mass accumulation could be because of dysregulation of leptin signalling in skeletal muscle. Thus, we investigated total protein expression and phosphorylation levels of the long isoform of the leptin receptor (OB‐Rb), and leptin signalling through janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3), insulin receptor substrate 1 (IRS‐1), AMP‐activated protein kinase (AMPK) and acetyl‐coenzyme A carboxylase (ACC), combined with the leptin signalling inhibitors suppressor of cytokine signalling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) in human skeletal muscle of different age.
Methods
Vastus lateralis muscle biopsies were obtained from 39 men matched for BMI < 30 kg m−2 and separated into three groups: 13 young (Y, 24 ± 4 years); 14 middle aged (MA, 44 ± 5 years) and 12 aged (A, 58 ± 8 years) subjects.
Results
Whole body fat percentage and plasma leptin were higher (P < 0.05), whereas lean mass, plasma free testosterone and total testosterone were lower (P < 0.05) in A compared to Y. Skeletal muscle OB‐Rb (170 KDa) protein expression and pTyr1141‐OB‐R170 were comparable between groups, whereas pTyr985‐OB‐R170 was lower in A compared to Y (P < 0.05). pSTAT3 levels tended (P = 0.09) to be lower (50%) in A compared to Y. In A, muscle PTP1B was greater and IRS‐1 lower than Y and MA respectively (P < 0.05). PTyr612‐IRS‐1 tended to be lower in A than in Y (P = 0.09). Suppressor of cytokine signalling 3 (SOCS3) protein expression, pJAK2, pSer1101‐IRS‐1, pAMPKα and pACCβ were similar between groups.
Conclusion
Age is associated with dysregulation of the leptin signalling and increased PTP1B protein expression in skeletal muscle.</description><identifier>ISSN: 1748-1708</identifier><identifier>EISSN: 1748-1716</identifier><identifier>DOI: 10.1111/apha.12273</identifier><identifier>PMID: 24605926</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adenylate Kinase - metabolism ; Adult ; age ; Age Factors ; Aged ; androgens ; Humans ; insulin receptor substrate 1 ; Insulin Receptor Substrate Proteins - metabolism ; Janus Kinase 2 - metabolism ; Leptin - pharmacology ; leptin resistance ; Male ; Middle Aged ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; Phosphorylation - drug effects ; protein tyrosine phosphatase 1B ; Receptors, Leptin - metabolism ; Signal Transduction - drug effects ; skeletal muscle ; STAT3 Transcription Factor - metabolism ; Suppressor of Cytokine Signaling 3 Protein ; Suppressor of Cytokine Signaling Proteins - metabolism ; Young Adult</subject><ispartof>Acta Physiologica, 2014-05, Vol.211 (1), p.214-228</ispartof><rights>2014 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd</rights><rights>2014 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2014 Scandinavian Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4313-a59dd28d6e7b063cd2dc9430bcd14bd8118d2e35ad0bba9aca9d05a69c5171793</citedby><cites>FETCH-LOGICAL-c4313-a59dd28d6e7b063cd2dc9430bcd14bd8118d2e35ad0bba9aca9d05a69c5171793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fapha.12273$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fapha.12273$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24605926$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guadalupe-Grau, A.</creatorcontrib><creatorcontrib>Larsen, S.</creatorcontrib><creatorcontrib>Guerra, B.</creatorcontrib><creatorcontrib>Calbet, J. A. L.</creatorcontrib><creatorcontrib>Dela, F.</creatorcontrib><creatorcontrib>Helge, J. W.</creatorcontrib><title>Influence of age on leptin induced skeletal muscle signalling</title><title>Acta Physiologica</title><addtitle>Acta Physiol</addtitle><description>Aim
Age associated fat mass accumulation could be because of dysregulation of leptin signalling in skeletal muscle. Thus, we investigated total protein expression and phosphorylation levels of the long isoform of the leptin receptor (OB‐Rb), and leptin signalling through janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3), insulin receptor substrate 1 (IRS‐1), AMP‐activated protein kinase (AMPK) and acetyl‐coenzyme A carboxylase (ACC), combined with the leptin signalling inhibitors suppressor of cytokine signalling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) in human skeletal muscle of different age.
Methods
Vastus lateralis muscle biopsies were obtained from 39 men matched for BMI < 30 kg m−2 and separated into three groups: 13 young (Y, 24 ± 4 years); 14 middle aged (MA, 44 ± 5 years) and 12 aged (A, 58 ± 8 years) subjects.
Results
Whole body fat percentage and plasma leptin were higher (P < 0.05), whereas lean mass, plasma free testosterone and total testosterone were lower (P < 0.05) in A compared to Y. Skeletal muscle OB‐Rb (170 KDa) protein expression and pTyr1141‐OB‐R170 were comparable between groups, whereas pTyr985‐OB‐R170 was lower in A compared to Y (P < 0.05). pSTAT3 levels tended (P = 0.09) to be lower (50%) in A compared to Y. In A, muscle PTP1B was greater and IRS‐1 lower than Y and MA respectively (P < 0.05). PTyr612‐IRS‐1 tended to be lower in A than in Y (P = 0.09). Suppressor of cytokine signalling 3 (SOCS3) protein expression, pJAK2, pSer1101‐IRS‐1, pAMPKα and pACCβ were similar between groups.
Conclusion
Age is associated with dysregulation of the leptin signalling and increased PTP1B protein expression in skeletal muscle.</description><subject>Adenylate Kinase - metabolism</subject><subject>Adult</subject><subject>age</subject><subject>Age Factors</subject><subject>Aged</subject><subject>androgens</subject><subject>Humans</subject><subject>insulin receptor substrate 1</subject><subject>Insulin Receptor Substrate Proteins - metabolism</subject><subject>Janus Kinase 2 - metabolism</subject><subject>Leptin - pharmacology</subject><subject>leptin resistance</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Phosphorylation - drug effects</subject><subject>protein tyrosine phosphatase 1B</subject><subject>Receptors, Leptin - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>skeletal muscle</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Suppressor of Cytokine Signaling 3 Protein</subject><subject>Suppressor of Cytokine Signaling Proteins - metabolism</subject><subject>Young Adult</subject><issn>1748-1708</issn><issn>1748-1716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90EFLwzAUB_Agio7pxQ8gBS8idOYlbdIePIyhUxnqRBG8hDR5m3VZO5sV3be3s7qDB3N5Ofzen-RPyCHQHjTnTC9edQ8Yk3yLdEBGSQgSxPbmTpM9cuD9G6UUGPCIsV2yxyJB45SJDjm_LiauxsJgUE4CPW1GEThcLPMiyAtbG7SBn6HDpXbBvPbGYeDzaaGdy4vpPtmZaOfx4Gd2ydPlxePgKhzdDa8H_VFoIg481HFqLUusQJlRwY1l1qQRp5mxEGU2AUgsQx5rS7NMp9ro1NJYi9TEzWdkyrvkpM1dVOV7jX6p5rk36JwusKy9ghgSwUBQ2dDjP_StrKvmvd9KxpHkgjbqtFWmKr2vcKIWVT7X1UoBVete1bpX9d1rg49-IutsjnZDf1tsALTgI3e4-idK9e-v-r-hYbuT-yV-bnZ0NVNCchmr59uheknl5c1w_KDG_As57ZAb</recordid><startdate>201405</startdate><enddate>201405</enddate><creator>Guadalupe-Grau, A.</creator><creator>Larsen, S.</creator><creator>Guerra, B.</creator><creator>Calbet, J. A. L.</creator><creator>Dela, F.</creator><creator>Helge, J. W.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TS</scope><scope>7X8</scope></search><sort><creationdate>201405</creationdate><title>Influence of age on leptin induced skeletal muscle signalling</title><author>Guadalupe-Grau, A. ; Larsen, S. ; Guerra, B. ; Calbet, J. A. L. ; Dela, F. ; Helge, J. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4313-a59dd28d6e7b063cd2dc9430bcd14bd8118d2e35ad0bba9aca9d05a69c5171793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenylate Kinase - metabolism</topic><topic>Adult</topic><topic>age</topic><topic>Age Factors</topic><topic>Aged</topic><topic>androgens</topic><topic>Humans</topic><topic>insulin receptor substrate 1</topic><topic>Insulin Receptor Substrate Proteins - metabolism</topic><topic>Janus Kinase 2 - metabolism</topic><topic>Leptin - pharmacology</topic><topic>leptin resistance</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Phosphorylation - drug effects</topic><topic>protein tyrosine phosphatase 1B</topic><topic>Receptors, Leptin - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>skeletal muscle</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Suppressor of Cytokine Signaling 3 Protein</topic><topic>Suppressor of Cytokine Signaling Proteins - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guadalupe-Grau, A.</creatorcontrib><creatorcontrib>Larsen, S.</creatorcontrib><creatorcontrib>Guerra, B.</creatorcontrib><creatorcontrib>Calbet, J. A. L.</creatorcontrib><creatorcontrib>Dela, F.</creatorcontrib><creatorcontrib>Helge, J. W.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>MEDLINE - Academic</collection><jtitle>Acta Physiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guadalupe-Grau, A.</au><au>Larsen, S.</au><au>Guerra, B.</au><au>Calbet, J. A. L.</au><au>Dela, F.</au><au>Helge, J. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of age on leptin induced skeletal muscle signalling</atitle><jtitle>Acta Physiologica</jtitle><addtitle>Acta Physiol</addtitle><date>2014-05</date><risdate>2014</risdate><volume>211</volume><issue>1</issue><spage>214</spage><epage>228</epage><pages>214-228</pages><issn>1748-1708</issn><eissn>1748-1716</eissn><abstract>Aim
Age associated fat mass accumulation could be because of dysregulation of leptin signalling in skeletal muscle. Thus, we investigated total protein expression and phosphorylation levels of the long isoform of the leptin receptor (OB‐Rb), and leptin signalling through janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3), insulin receptor substrate 1 (IRS‐1), AMP‐activated protein kinase (AMPK) and acetyl‐coenzyme A carboxylase (ACC), combined with the leptin signalling inhibitors suppressor of cytokine signalling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) in human skeletal muscle of different age.
Methods
Vastus lateralis muscle biopsies were obtained from 39 men matched for BMI < 30 kg m−2 and separated into three groups: 13 young (Y, 24 ± 4 years); 14 middle aged (MA, 44 ± 5 years) and 12 aged (A, 58 ± 8 years) subjects.
Results
Whole body fat percentage and plasma leptin were higher (P < 0.05), whereas lean mass, plasma free testosterone and total testosterone were lower (P < 0.05) in A compared to Y. Skeletal muscle OB‐Rb (170 KDa) protein expression and pTyr1141‐OB‐R170 were comparable between groups, whereas pTyr985‐OB‐R170 was lower in A compared to Y (P < 0.05). pSTAT3 levels tended (P = 0.09) to be lower (50%) in A compared to Y. In A, muscle PTP1B was greater and IRS‐1 lower than Y and MA respectively (P < 0.05). PTyr612‐IRS‐1 tended to be lower in A than in Y (P = 0.09). Suppressor of cytokine signalling 3 (SOCS3) protein expression, pJAK2, pSer1101‐IRS‐1, pAMPKα and pACCβ were similar between groups.
Conclusion
Age is associated with dysregulation of the leptin signalling and increased PTP1B protein expression in skeletal muscle.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24605926</pmid><doi>10.1111/apha.12273</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adenylate Kinase - metabolism Adult age Age Factors Aged androgens Humans insulin receptor substrate 1 Insulin Receptor Substrate Proteins - metabolism Janus Kinase 2 - metabolism Leptin - pharmacology leptin resistance Male Middle Aged Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Phosphorylation - drug effects protein tyrosine phosphatase 1B Receptors, Leptin - metabolism Signal Transduction - drug effects skeletal muscle STAT3 Transcription Factor - metabolism Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins - metabolism Young Adult |
| title | Influence of age on leptin induced skeletal muscle signalling |
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