FLP Recombinase of the 2 μm circle plasmid of Saccharomyces cerevisiae bends its DNA target : Isolation of FLP mutants defective in DNA bending

The FLP recombinase is encoded by the yeast plasmid 2 μm circle and catalyses a site-specific recombination reaction that results in inversion of a segment of the 2 μm plasmid. We describe a method for the isolation of inactivating mutations in the FLP gene. The analysis of the recombination and bin...

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Veröffentlicht in:	Journal of molecular biology 1989-02, Vol.205 (4), p.647-658
Hauptverfasser:	Schwartz, Carol J.E., Sadowski, Paul D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Saccharomyces cerevisiae Thallophyta, bryophyta Vegetals
Online-Zugang:	Volltext
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