Activation of α1-adrenoceptors enhances excitatory synaptic transmission via a pre- and postsynaptic protein kinase C-dependent mechanism in the medial prefrontal cortex of rats
The physiological effects of α1‐adrenoceptors (α1‐ARs) have been examined in many brain regions. However, little is known about the mechanism of modulation on synaptic transmission by α1‐ARs in the medial prefrontal cortex (mPFC). The present study investigated how α1‐AR activation regulates glutama...
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| Veröffentlicht in: | The European journal of neuroscience 2014-04, Vol.39 (8), p.1281-1293 |
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| creator | Luo, Fei Tang, Hua Li, Bao-ming Li, Si-hai |
| description | The physiological effects of α1‐adrenoceptors (α1‐ARs) have been examined in many brain regions. However, little is known about the mechanism of modulation on synaptic transmission by α1‐ARs in the medial prefrontal cortex (mPFC). The present study investigated how α1‐AR activation regulates glutamatergic synaptic transmission in layer V/VI pyramidal cells of the rat mPFC. We found that the α1‐AR agonist phenylephrine (Phe) induced a significant enhancement of the amplitude and frequency of miniature excitatory postsynaptic currents (mEPSCs). The facilitation effect of Phe on the frequency of mEPSCs involved a presynaptic protein kinase C‐dependent pathway. Phe produced a significant enhancement on the amplitude of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor (AMPA‐R)‐ and N‐methyl‐d‐aspartic acid receptor (NMDA‐R)‐mediated evoked excitatory postsynaptic currents (eEPSCs). Phe enhanced inward currents evoked by puff application of glutamate or NMDA. The Phe‐induced facilitation of AMPA‐R‐ and NMDA‐R‐mediated eEPSCs required, in part, postsynaptic Gq, phospholipase C and PKC. These findings suggest that α1‐AR activation facilitates excitatory synaptic transmission in mPFC pyramidal cells via both pre‐ and post‐synaptic PKC‐dependent mechanisms.
Little is known about the mechanism of modulation on synaptic transmission by α1‐AR in the medial prefrontal cortex (mPFC). We found that α1‐AR activation enhances glutamatergic transmission in the mPFC by increasing the glutamate release from presynaptic terminals, via PKC‐dependent mechanism, and by enhancing the responsiveness of postsynaptic AMPA‐Rs and NMDA‐Rs, likely via Gq/PLC/PKC signaling pathway. |
| doi_str_mv | 10.1111/ejn.12495 |
| format | Article |
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Little is known about the mechanism of modulation on synaptic transmission by α1‐AR in the medial prefrontal cortex (mPFC). We found that α1‐AR activation enhances glutamatergic transmission in the mPFC by increasing the glutamate release from presynaptic terminals, via PKC‐dependent mechanism, and by enhancing the responsiveness of postsynaptic AMPA‐Rs and NMDA‐Rs, likely via Gq/PLC/PKC signaling pathway.</description><identifier>ISSN: 0953-816X</identifier><identifier>EISSN: 1460-9568</identifier><identifier>DOI: 10.1111/ejn.12495</identifier><identifier>PMID: 24494713</identifier><language>eng</language><publisher>France: Blackwell Publishing Ltd</publisher><subject>Adrenergic alpha-1 Receptor Agonists - pharmacology ; Animals ; Excitatory Postsynaptic Potentials ; excitatory synaptic transmission ; medial prefrontal cortex ; Miniature Postsynaptic Potentials ; Phenylephrine - pharmacology ; Prefrontal Cortex - cytology ; Prefrontal Cortex - metabolism ; Prefrontal Cortex - physiology ; Protein Kinase C - metabolism ; Pyramidal Cells - drug effects ; Pyramidal Cells - metabolism ; Pyramidal Cells - physiology ; rat ; Rats ; Rats, Sprague-Dawley ; Receptors, Adrenergic, alpha-1 - metabolism ; Receptors, AMPA - metabolism ; Receptors, N-Methyl-D-Aspartate - metabolism ; Synapses - metabolism ; Synapses - physiology ; α1-Adrenoceptor</subject><ispartof>The European journal of neuroscience, 2014-04, Vol.39 (8), p.1281-1293</ispartof><rights>2014 Federation of European Neuroscience Societies and John Wiley & Sons Ltd</rights><rights>2014 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3445-b626a454deb104d5cd669ccd9ebaed28308d0758ab4f40d09a3bb7dc1ab35bb13</citedby><cites>FETCH-LOGICAL-c3445-b626a454deb104d5cd669ccd9ebaed28308d0758ab4f40d09a3bb7dc1ab35bb13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fejn.12495$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fejn.12495$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24494713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Fei</creatorcontrib><creatorcontrib>Tang, Hua</creatorcontrib><creatorcontrib>Li, Bao-ming</creatorcontrib><creatorcontrib>Li, Si-hai</creatorcontrib><title>Activation of α1-adrenoceptors enhances excitatory synaptic transmission via a pre- and postsynaptic protein kinase C-dependent mechanism in the medial prefrontal cortex of rats</title><title>The European journal of neuroscience</title><addtitle>Eur J Neurosci</addtitle><description>The physiological effects of α1‐adrenoceptors (α1‐ARs) have been examined in many brain regions. However, little is known about the mechanism of modulation on synaptic transmission by α1‐ARs in the medial prefrontal cortex (mPFC). The present study investigated how α1‐AR activation regulates glutamatergic synaptic transmission in layer V/VI pyramidal cells of the rat mPFC. We found that the α1‐AR agonist phenylephrine (Phe) induced a significant enhancement of the amplitude and frequency of miniature excitatory postsynaptic currents (mEPSCs). The facilitation effect of Phe on the frequency of mEPSCs involved a presynaptic protein kinase C‐dependent pathway. Phe produced a significant enhancement on the amplitude of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor (AMPA‐R)‐ and N‐methyl‐d‐aspartic acid receptor (NMDA‐R)‐mediated evoked excitatory postsynaptic currents (eEPSCs). Phe enhanced inward currents evoked by puff application of glutamate or NMDA. The Phe‐induced facilitation of AMPA‐R‐ and NMDA‐R‐mediated eEPSCs required, in part, postsynaptic Gq, phospholipase C and PKC. These findings suggest that α1‐AR activation facilitates excitatory synaptic transmission in mPFC pyramidal cells via both pre‐ and post‐synaptic PKC‐dependent mechanisms.
Little is known about the mechanism of modulation on synaptic transmission by α1‐AR in the medial prefrontal cortex (mPFC). We found that α1‐AR activation enhances glutamatergic transmission in the mPFC by increasing the glutamate release from presynaptic terminals, via PKC‐dependent mechanism, and by enhancing the responsiveness of postsynaptic AMPA‐Rs and NMDA‐Rs, likely via Gq/PLC/PKC signaling pathway.</description><subject>Adrenergic alpha-1 Receptor Agonists - pharmacology</subject><subject>Animals</subject><subject>Excitatory Postsynaptic Potentials</subject><subject>excitatory synaptic transmission</subject><subject>medial prefrontal cortex</subject><subject>Miniature Postsynaptic Potentials</subject><subject>Phenylephrine - pharmacology</subject><subject>Prefrontal Cortex - cytology</subject><subject>Prefrontal Cortex - metabolism</subject><subject>Prefrontal Cortex - physiology</subject><subject>Protein Kinase C - metabolism</subject><subject>Pyramidal Cells - drug effects</subject><subject>Pyramidal Cells - metabolism</subject><subject>Pyramidal Cells - physiology</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Adrenergic, alpha-1 - metabolism</subject><subject>Receptors, AMPA - metabolism</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>Synapses - metabolism</subject><subject>Synapses - physiology</subject><subject>α1-Adrenoceptor</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9uEzEQxi0EoqFw4AWQj3DY1t61vbvHKmoLqCoC8e9mje2J6jaxF9spzWPBg_BMOKTNDV88Gv_m88x8hLzk7IjXc4zX4Yi3YpSPyIwLxZpRquExmbFRds3A1fcD8izna8bYoIR8Sg5aIUbR825Gfp_Y4m-h-BhoXNA_v3gDLmGIFqcSU6YYriBYrMGd9QVqbkPzJsBUvKUlQcgrn_O2_NYDBTolbCgER6eYyx6cUizoA73xATLSeeNwwuAwFLpCW3_weUXre7nCmnAelluhRYqh1NDGVPBu21-Ckp-TJwtYZnxxfx-SL2enn-dvm4sP5-_mJxeN7YSQjVGtAiGFQ8OZcNI6pUZr3YgG0LVDxwbHejmAEQvBHBuhM6Z3loPppDG8OySvd7q1-R9rzEXXSS0ulxAwrrPmkg-KD3XtFX2zQ22KOdfG9ZT8CtJGc6a3Fulqkf5nUWVf3cuuTR11Tz54UoHjHfDTL3HzfyV9-v7yQbLZVfhc97SvgHSjVd_1Un-7PNft2aePchSj_tr9BYjcsEw</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Luo, Fei</creator><creator>Tang, Hua</creator><creator>Li, Bao-ming</creator><creator>Li, Si-hai</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Activation of α1-adrenoceptors enhances excitatory synaptic transmission via a pre- and postsynaptic protein kinase C-dependent mechanism in the medial prefrontal cortex of rats</title><author>Luo, Fei ; Tang, Hua ; Li, Bao-ming ; Li, Si-hai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3445-b626a454deb104d5cd669ccd9ebaed28308d0758ab4f40d09a3bb7dc1ab35bb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adrenergic alpha-1 Receptor Agonists - pharmacology</topic><topic>Animals</topic><topic>Excitatory Postsynaptic Potentials</topic><topic>excitatory synaptic transmission</topic><topic>medial prefrontal cortex</topic><topic>Miniature Postsynaptic Potentials</topic><topic>Phenylephrine - pharmacology</topic><topic>Prefrontal Cortex - cytology</topic><topic>Prefrontal Cortex - metabolism</topic><topic>Prefrontal Cortex - physiology</topic><topic>Protein Kinase C - metabolism</topic><topic>Pyramidal Cells - drug effects</topic><topic>Pyramidal Cells - metabolism</topic><topic>Pyramidal Cells - physiology</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Adrenergic, alpha-1 - metabolism</topic><topic>Receptors, AMPA - metabolism</topic><topic>Receptors, N-Methyl-D-Aspartate - metabolism</topic><topic>Synapses - metabolism</topic><topic>Synapses - physiology</topic><topic>α1-Adrenoceptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Fei</creatorcontrib><creatorcontrib>Tang, Hua</creatorcontrib><creatorcontrib>Li, Bao-ming</creatorcontrib><creatorcontrib>Li, Si-hai</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Fei</au><au>Tang, Hua</au><au>Li, Bao-ming</au><au>Li, Si-hai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of α1-adrenoceptors enhances excitatory synaptic transmission via a pre- and postsynaptic protein kinase C-dependent mechanism in the medial prefrontal cortex of rats</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>2014-04</date><risdate>2014</risdate><volume>39</volume><issue>8</issue><spage>1281</spage><epage>1293</epage><pages>1281-1293</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>The physiological effects of α1‐adrenoceptors (α1‐ARs) have been examined in many brain regions. However, little is known about the mechanism of modulation on synaptic transmission by α1‐ARs in the medial prefrontal cortex (mPFC). The present study investigated how α1‐AR activation regulates glutamatergic synaptic transmission in layer V/VI pyramidal cells of the rat mPFC. We found that the α1‐AR agonist phenylephrine (Phe) induced a significant enhancement of the amplitude and frequency of miniature excitatory postsynaptic currents (mEPSCs). The facilitation effect of Phe on the frequency of mEPSCs involved a presynaptic protein kinase C‐dependent pathway. Phe produced a significant enhancement on the amplitude of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor (AMPA‐R)‐ and N‐methyl‐d‐aspartic acid receptor (NMDA‐R)‐mediated evoked excitatory postsynaptic currents (eEPSCs). Phe enhanced inward currents evoked by puff application of glutamate or NMDA. The Phe‐induced facilitation of AMPA‐R‐ and NMDA‐R‐mediated eEPSCs required, in part, postsynaptic Gq, phospholipase C and PKC. These findings suggest that α1‐AR activation facilitates excitatory synaptic transmission in mPFC pyramidal cells via both pre‐ and post‐synaptic PKC‐dependent mechanisms.
Little is known about the mechanism of modulation on synaptic transmission by α1‐AR in the medial prefrontal cortex (mPFC). We found that α1‐AR activation enhances glutamatergic transmission in the mPFC by increasing the glutamate release from presynaptic terminals, via PKC‐dependent mechanism, and by enhancing the responsiveness of postsynaptic AMPA‐Rs and NMDA‐Rs, likely via Gq/PLC/PKC signaling pathway.</abstract><cop>France</cop><pub>Blackwell Publishing Ltd</pub><pmid>24494713</pmid><doi>10.1111/ejn.12495</doi><tpages>13</tpages></addata></record> |
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| subjects | Adrenergic alpha-1 Receptor Agonists - pharmacology Animals Excitatory Postsynaptic Potentials excitatory synaptic transmission medial prefrontal cortex Miniature Postsynaptic Potentials Phenylephrine - pharmacology Prefrontal Cortex - cytology Prefrontal Cortex - metabolism Prefrontal Cortex - physiology Protein Kinase C - metabolism Pyramidal Cells - drug effects Pyramidal Cells - metabolism Pyramidal Cells - physiology rat Rats Rats, Sprague-Dawley Receptors, Adrenergic, alpha-1 - metabolism Receptors, AMPA - metabolism Receptors, N-Methyl-D-Aspartate - metabolism Synapses - metabolism Synapses - physiology α1-Adrenoceptor |
| title | Activation of α1-adrenoceptors enhances excitatory synaptic transmission via a pre- and postsynaptic protein kinase C-dependent mechanism in the medial prefrontal cortex of rats |
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