Synergistic effect of tumor necrosis factor-α and interferon-γ on collagen synthesis of human skin fibroblasts in vitro
The effect of tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ) on collagen metabolism by human diploid fibroblasts in confluent monolayer culture was examined. Recombinant TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold, while recombinant IFNγ affected on...
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| Veröffentlicht in: | Experimental cell research 1989-04, Vol.181 (2), p.409-419 |
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| container_title | Experimental cell research |
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| creator | Scharffetter, Karin Heckmann, Marc Hatamochi, Atsushi Mauch, Cornelia Stein, Bernd Riethmüller, Gert Ziegler-Heitbrock, H.-W.Löms Krieg, Thomas |
| description | The effect of tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ) on collagen metabolism by human diploid fibroblasts in confluent monolayer culture was examined. Recombinant TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold, while recombinant IFNγ affected only collagen mRNA levels. The combination of TNFα (10 ng/ml) and IFNγ (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of collagen mRNA levels indicating that the two cytokines act synergistically. In contrast no such synergism was observed with respect to collagenase mRNA levels. The effect of TNFα and IFNγ on collagen metabolism reported here indicates a complex interaction of different cytokines in the control of tissue remodeling that occurs during inflammation, repair, or atrophy. |
| doi_str_mv | 10.1016/0014-4827(89)90098-0 |
| format | Article |
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Recombinant TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold, while recombinant IFNγ affected only collagen mRNA levels. The combination of TNFα (10 ng/ml) and IFNγ (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of collagen mRNA levels indicating that the two cytokines act synergistically. In contrast no such synergism was observed with respect to collagenase mRNA levels. The effect of TNFα and IFNγ on collagen metabolism reported here indicates a complex interaction of different cytokines in the control of tissue remodeling that occurs during inflammation, repair, or atrophy.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(89)90098-0</identifier><identifier>PMID: 2538336</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Cell physiology ; Cells, Cultured ; Collagen - biosynthesis ; Collagen - genetics ; Drug Synergism ; Fibroblasts ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Humans ; Interferon-gamma - pharmacology ; Microbial Collagenase - genetics ; Molecular and cellular biology ; Recombinant Proteins - pharmacology ; Responses to growth factors, tumor promotors, other factors ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Experimental cell research, 1989-04, Vol.181 (2), p.409-419</ispartof><rights>1989</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-737ad0b0c5630aaf8e5948069019d6874092e0c643aab0698c020926ab48078a3</citedby><cites>FETCH-LOGICAL-c333t-737ad0b0c5630aaf8e5948069019d6874092e0c643aab0698c020926ab48078a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(89)90098-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19311579$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2538336$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scharffetter, Karin</creatorcontrib><creatorcontrib>Heckmann, Marc</creatorcontrib><creatorcontrib>Hatamochi, Atsushi</creatorcontrib><creatorcontrib>Mauch, Cornelia</creatorcontrib><creatorcontrib>Stein, Bernd</creatorcontrib><creatorcontrib>Riethmüller, Gert</creatorcontrib><creatorcontrib>Ziegler-Heitbrock, H.-W.Löms</creatorcontrib><creatorcontrib>Krieg, Thomas</creatorcontrib><title>Synergistic effect of tumor necrosis factor-α and interferon-γ on collagen synthesis of human skin fibroblasts in vitro</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The effect of tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ) on collagen metabolism by human diploid fibroblasts in confluent monolayer culture was examined. Recombinant TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold, while recombinant IFNγ affected only collagen mRNA levels. The combination of TNFα (10 ng/ml) and IFNγ (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of collagen mRNA levels indicating that the two cytokines act synergistically. In contrast no such synergism was observed with respect to collagenase mRNA levels. The effect of TNFα and IFNγ on collagen metabolism reported here indicates a complex interaction of different cytokines in the control of tissue remodeling that occurs during inflammation, repair, or atrophy.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Collagen - biosynthesis</subject><subject>Collagen - genetics</subject><subject>Drug Synergism</subject><subject>Fibroblasts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Interferon-gamma - pharmacology</subject><subject>Microbial Collagenase - genetics</subject><subject>Molecular and cellular biology</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQhS0EKpfCG4DkDQgWgXGcOPamEqr4kyqxANaW44xbQ2IX26l0HwvxHn0mHO5V2bGydOY7RzPHhDxl8JoBE28AWNd0sh1eSvVKASjZwD2yY6Cgabu2vU92d8hD8ijn7wAgJRMn5KTtueRc7Mj-yz5guvS5eEvRObSFRkfLusREA9oUs8_UGVtiam5_URMm6kPB5DDF0Nz-pjFQG-fZXGKgeR_KFW6OmnG1LqZKP3ygzo8pjrPJJVc3vfElxcfkgTNzxifH95R8e__u6_nH5uLzh0_nby8ayzkvzcAHM8EIthccjHESe9VJEAqYmoQcOlAtghUdN2assrTQVkmYsVKDNPyUvDjkXqf4c8Vc9OKzxbpxwLhmzXomuRR9BbsDuB2dEzp9nfxi0l4z0FvjeqtTb3VqqfTfxjVU27Nj_jouON2ZjhXX-fPj3GRrZpdMsD7_y1acsX5QlTs7cFjLuPGYdLYeg8XJp_oreor-_4v8AeTunuA</recordid><startdate>198904</startdate><enddate>198904</enddate><creator>Scharffetter, Karin</creator><creator>Heckmann, Marc</creator><creator>Hatamochi, Atsushi</creator><creator>Mauch, Cornelia</creator><creator>Stein, Bernd</creator><creator>Riethmüller, Gert</creator><creator>Ziegler-Heitbrock, H.-W.Löms</creator><creator>Krieg, Thomas</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>198904</creationdate><title>Synergistic effect of tumor necrosis factor-α and interferon-γ on collagen synthesis of human skin fibroblasts in vitro</title><author>Scharffetter, Karin ; Heckmann, Marc ; Hatamochi, Atsushi ; Mauch, Cornelia ; Stein, Bernd ; Riethmüller, Gert ; Ziegler-Heitbrock, H.-W.Löms ; Krieg, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-737ad0b0c5630aaf8e5948069019d6874092e0c643aab0698c020926ab48078a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Collagen - biosynthesis</topic><topic>Collagen - genetics</topic><topic>Drug Synergism</topic><topic>Fibroblasts</topic><topic>Fundamental and applied biological sciences. 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Recombinant TNFα reduced collagen mRNA levels 2-fold and stimulated collagenase mRNA levels 5-fold, while recombinant IFNγ affected only collagen mRNA levels. The combination of TNFα (10 ng/ml) and IFNγ (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of collagen mRNA levels indicating that the two cytokines act synergistically. In contrast no such synergism was observed with respect to collagenase mRNA levels. The effect of TNFα and IFNγ on collagen metabolism reported here indicates a complex interaction of different cytokines in the control of tissue remodeling that occurs during inflammation, repair, or atrophy.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>2538336</pmid><doi>10.1016/0014-4827(89)90098-0</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental cell research, 1989-04, Vol.181 (2), p.409-419 |
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| subjects | Adult Biological and medical sciences Cell physiology Cells, Cultured Collagen - biosynthesis Collagen - genetics Drug Synergism Fibroblasts Fundamental and applied biological sciences. Psychology Gene Expression Regulation Humans Interferon-gamma - pharmacology Microbial Collagenase - genetics Molecular and cellular biology Recombinant Proteins - pharmacology Responses to growth factors, tumor promotors, other factors RNA, Messenger - analysis RNA, Messenger - genetics Tumor Necrosis Factor-alpha - pharmacology |
| title | Synergistic effect of tumor necrosis factor-α and interferon-γ on collagen synthesis of human skin fibroblasts in vitro |
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