Development of a multiplex RT-PCR-ELISA to identify four distinct species of tospovirus

•A multiplex RT-PCR-ELISA for identifying four distinct species of tospovirus was developed.•The developed multiplex RT-PCR-ELISA was 10–1000 times more sensitive for tospovirus identification in field-collected plant samples than conventional RT-PCR.•The developed multiplex RT-PCR-ELISA assay can b...

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Veröffentlicht in:	Journal of virological methods 2014-06, Vol.202, p.54-63
Hauptverfasser:	Charoenvilaisiri, Saengsoon, Seepiban, Channarong, Bhunchoth, Anjana, Warin, Nuchnard, Luxananil, Plearnpis, Gajanandana, Oraprapai
Format:	Artikel
Sprache:	eng
Schlagworte:	Enzyme-Linked Immunosorbent Assay - methods High-Throughput Screening Assays Multiplex Multiplex Polymerase Chain Reaction - methods Nucleic Acid Hybridization Nucleocapsid - genetics Nucleocapsid gene Plants Reverse Transcriptase Polymerase Chain Reaction - methods RT-PCR-ELISA Sensitivity and Specificity Thailand Tospovirus Tospovirus - classification Tospovirus - genetics Tospovirus - isolation & purification
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