A new nanoPCR molecular assay for detection of porcine bocavirus

•NanoPCR is a novel method for amplifying DNA; here a nanoPCR assay was developed to detect PBoV.•The nanoPCR assay was 100-fold more sensitive than a conventional PCR assay.•PBoV positive rate was 71.3% by PBoV nanoPCR which demonstrated PBoV is epidemic in China. Nanoparticle-assisted polymerase c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of virological methods 2014-06, Vol.202, p.106-111
Hauptverfasser:	Wang, Xiaoling, Bai, Aiquan, Zhang, Jing, Kong, Miaomiao, Cui, Yuchao, Ma, Xingjie, Ai, Xia, Tang, Qinghai, Cui, Shangjin
Format:	Artikel
Sprache:	eng
Schlagworte:	African swine fever virus Animals Bocaparvovirus Bocavirus - genetics Bocavirus - isolation & purification Cardiovirus A Classical swine fever virus complementary DNA Detection detection limit DNA Primers - genetics Epidemic characterization epidemiology NanoPCR NS1 gene nucleotide sequences Parvoviridae Infections - diagnosis Parvoviridae Infections - veterinary Parvoviridae Infections - virology PBoV plasmids polymerase chain reaction Polymerase Chain Reaction - methods Porcine reproductive and respiratory syndrome virus Sensitivity and Specificity Suid alphaherpesvirus 1 Swine Swine Diseases - diagnosis Swine Diseases - virology Teschovirus A Ungulate protoparvovirus 1 Viral Nonstructural Proteins - genetics viruses
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!