Corrosive and Cytotoxic Properties of Compact Specimens and Microparticles of Ni-Cr Dental Alloy
Purpose Nickel‐chromium (Ni‐Cr) dental alloys have been widely used in prosthodontic practice, but there is a permanent concern about their biocompatibility due to the release of metal ions. This is especially important when Ni‐Cr metal microparticles are incorporated into gingival tissue during pro...
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| Veröffentlicht in: | Journal of prosthodontics 2014-04, Vol.23 (3), p.221-226 |
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| creator | Ristic, Ljubisa Vucevic, Dragana Radovic, Ljubica Djordjevic, Snezana Nikacevic, Milutin Colic, Miodrag |
| description | Purpose
Nickel‐chromium (Ni‐Cr) dental alloys have been widely used in prosthodontic practice, but there is a permanent concern about their biocompatibility due to the release of metal ions. This is especially important when Ni‐Cr metal microparticles are incorporated into gingival tissue during prosthodontic procedures. Therefore, the aim of this study was to examine and compare the corrosion and cytotoxic properties of compact specimens and microparticles of Ni‐Cr dental alloy.
Materials and Methods
Ni‐Cr alloy, Remanium CSe bars (4 mm diameter), were made by the standard casting method and then cut into 0.5‐mm‐thick disks. Metal particles were obtained by scraping the bars using a diamond instrument for crown preparation. The microstructure was observed by an optical microscope. Quantitative determination and morphological and dimensional characterization of metal particles were carried out by a scanning electron microscope and Leica Application Suite software for image analysis. Corrosion was studied by conditioning the alloy specimens in the RPMI 1640 medium, containing 10% fetal calf serum in an incubator with 5% CO2 for 72 hours at 37°C. Inductively coupled plasma‐optical emission spectrometry was used to assess metal ion release. The cytotoxity of conditioning medium (CM) was investigated on L929 cells using an MTT test. One‐way ANOVA was used for statistical analysis.
Results
After casting, the microstructure of the Remanium CSe compact specimen composed of Ni, Cr, Mo, Si, Fe, Al, and Co had a typical dendritic structure. Alloy microparticles had an irregular shape with a wide size range: from less than 1 μm to more than 100 μm. The release of metal ions, especially Ni and Mo from microparticles, was significantly higher, compared to the compact alloy specimen. The CM prepared from compact alloy was not cytotoxic at any tested dilutions, whereas CM from alloy microparticles showed dose‐dependent cytotoxicity (90% CM and 45% CM versus control; p < 0.005).
Conclusion
Ni‐Cr microparticles showed less corrosion resistance and lower biocompatibility than compact alloy. This could affect health on long‐term exposure, especially in sensitized individuals. |
| doi_str_mv | 10.1111/jopr.12100 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1517877098</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3278473981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4610-e17b15e857827691ed02b8472d0bbfc689bd39f3ff754ae1703721784e77b65e3</originalsourceid><addsrcrecordid>eNp90MtO3DAUBmALFZVbN32AKlI3FVLAJ4ljZ4lSYEDcOqXq7FwnOZE8TeJgZ2Dm7TGTgQULvLEX3_l1_BPyFegR-HM8N709gggo3SK7wOIoFEk2--TflGVhlsBsh-w5N6cUgAn4THaiBEBACrvkX26sNU4_YqC6KshXgxnMUpfBnTU92kGjC0wd5KbtVTkEv3ssdYudW-trXXqlvCqb0d3oMLfBT-wG1QQnTWNWB2S7Vo3DL5t7n_w5O73PJ-HV7flFfnIVlkkKNETgBTAUjIuIpxlgRaNCJDyqaFHUZSqyooqzOq5rzhLlNY15BFwkyHmRMoz3yY8xt7fmYYFukK12JTaN6tAsnATmNec0E55-f0fnZmE7v91axTxjnHl1OCr_R-cs1rK3ulV2JYHKl97lS-9y3bvH3zaRi6LF6o2-Fu0BjOBJN7j6IEpe3t5NX0PDcUa7AZdvM8r-l6nfksm_N-eSplM2-zWJ5Cx-BlrFm3s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1517379575</pqid></control><display><type>article</type><title>Corrosive and Cytotoxic Properties of Compact Specimens and Microparticles of Ni-Cr Dental Alloy</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ristic, Ljubisa ; Vucevic, Dragana ; Radovic, Ljubica ; Djordjevic, Snezana ; Nikacevic, Milutin ; Colic, Miodrag</creator><creatorcontrib>Ristic, Ljubisa ; Vucevic, Dragana ; Radovic, Ljubica ; Djordjevic, Snezana ; Nikacevic, Milutin ; Colic, Miodrag</creatorcontrib><description>Purpose
Nickel‐chromium (Ni‐Cr) dental alloys have been widely used in prosthodontic practice, but there is a permanent concern about their biocompatibility due to the release of metal ions. This is especially important when Ni‐Cr metal microparticles are incorporated into gingival tissue during prosthodontic procedures. Therefore, the aim of this study was to examine and compare the corrosion and cytotoxic properties of compact specimens and microparticles of Ni‐Cr dental alloy.
Materials and Methods
Ni‐Cr alloy, Remanium CSe bars (4 mm diameter), were made by the standard casting method and then cut into 0.5‐mm‐thick disks. Metal particles were obtained by scraping the bars using a diamond instrument for crown preparation. The microstructure was observed by an optical microscope. Quantitative determination and morphological and dimensional characterization of metal particles were carried out by a scanning electron microscope and Leica Application Suite software for image analysis. Corrosion was studied by conditioning the alloy specimens in the RPMI 1640 medium, containing 10% fetal calf serum in an incubator with 5% CO2 for 72 hours at 37°C. Inductively coupled plasma‐optical emission spectrometry was used to assess metal ion release. The cytotoxity of conditioning medium (CM) was investigated on L929 cells using an MTT test. One‐way ANOVA was used for statistical analysis.
Results
After casting, the microstructure of the Remanium CSe compact specimen composed of Ni, Cr, Mo, Si, Fe, Al, and Co had a typical dendritic structure. Alloy microparticles had an irregular shape with a wide size range: from less than 1 μm to more than 100 μm. The release of metal ions, especially Ni and Mo from microparticles, was significantly higher, compared to the compact alloy specimen. The CM prepared from compact alloy was not cytotoxic at any tested dilutions, whereas CM from alloy microparticles showed dose‐dependent cytotoxicity (90% CM and 45% CM versus control; p < 0.005).
Conclusion
Ni‐Cr microparticles showed less corrosion resistance and lower biocompatibility than compact alloy. This could affect health on long‐term exposure, especially in sensitized individuals.</description><identifier>ISSN: 1059-941X</identifier><identifier>EISSN: 1532-849X</identifier><identifier>DOI: 10.1111/jopr.12100</identifier><identifier>PMID: 24118161</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>alloy ; Aluminum - chemistry ; Aluminum - toxicity ; Animals ; Cell Line ; Cell Shape - drug effects ; Cell Survival - drug effects ; Chromium - chemistry ; Chromium - toxicity ; Chromium Alloys - chemistry ; Chromium Alloys - toxicity ; Cobalt - chemistry ; Cobalt - toxicity ; Corrosion ; cytotoxicity ; Dental Casting Investment - chemistry ; Dental Casting Investment - toxicity ; Dentistry ; Diamond - chemistry ; Fibroblasts - drug effects ; Image Processing, Computer-Assisted - methods ; Iron - chemistry ; Iron - toxicity ; Materials Testing ; Mice ; microparticles ; Microscopy, Electron, Scanning ; Molybdenum - chemistry ; Molybdenum - toxicity ; Ni-Cr ; Nickel - chemistry ; Nickel - toxicity ; Particle Size ; Silicon - chemistry ; Silicon - toxicity ; Spectrophotometry, Atomic ; Surface Properties</subject><ispartof>Journal of prosthodontics, 2014-04, Vol.23 (3), p.221-226</ispartof><rights>2013 by the American College of Prosthodontists</rights><rights>2013 by the American College of Prosthodontists.</rights><rights>2014 American College of Prosthodontists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4610-e17b15e857827691ed02b8472d0bbfc689bd39f3ff754ae1703721784e77b65e3</citedby><cites>FETCH-LOGICAL-c4610-e17b15e857827691ed02b8472d0bbfc689bd39f3ff754ae1703721784e77b65e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjopr.12100$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjopr.12100$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24118161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ristic, Ljubisa</creatorcontrib><creatorcontrib>Vucevic, Dragana</creatorcontrib><creatorcontrib>Radovic, Ljubica</creatorcontrib><creatorcontrib>Djordjevic, Snezana</creatorcontrib><creatorcontrib>Nikacevic, Milutin</creatorcontrib><creatorcontrib>Colic, Miodrag</creatorcontrib><title>Corrosive and Cytotoxic Properties of Compact Specimens and Microparticles of Ni-Cr Dental Alloy</title><title>Journal of prosthodontics</title><addtitle>Journal of Prosthodontics</addtitle><description>Purpose
Nickel‐chromium (Ni‐Cr) dental alloys have been widely used in prosthodontic practice, but there is a permanent concern about their biocompatibility due to the release of metal ions. This is especially important when Ni‐Cr metal microparticles are incorporated into gingival tissue during prosthodontic procedures. Therefore, the aim of this study was to examine and compare the corrosion and cytotoxic properties of compact specimens and microparticles of Ni‐Cr dental alloy.
Materials and Methods
Ni‐Cr alloy, Remanium CSe bars (4 mm diameter), were made by the standard casting method and then cut into 0.5‐mm‐thick disks. Metal particles were obtained by scraping the bars using a diamond instrument for crown preparation. The microstructure was observed by an optical microscope. Quantitative determination and morphological and dimensional characterization of metal particles were carried out by a scanning electron microscope and Leica Application Suite software for image analysis. Corrosion was studied by conditioning the alloy specimens in the RPMI 1640 medium, containing 10% fetal calf serum in an incubator with 5% CO2 for 72 hours at 37°C. Inductively coupled plasma‐optical emission spectrometry was used to assess metal ion release. The cytotoxity of conditioning medium (CM) was investigated on L929 cells using an MTT test. One‐way ANOVA was used for statistical analysis.
Results
After casting, the microstructure of the Remanium CSe compact specimen composed of Ni, Cr, Mo, Si, Fe, Al, and Co had a typical dendritic structure. Alloy microparticles had an irregular shape with a wide size range: from less than 1 μm to more than 100 μm. The release of metal ions, especially Ni and Mo from microparticles, was significantly higher, compared to the compact alloy specimen. The CM prepared from compact alloy was not cytotoxic at any tested dilutions, whereas CM from alloy microparticles showed dose‐dependent cytotoxicity (90% CM and 45% CM versus control; p < 0.005).
Conclusion
Ni‐Cr microparticles showed less corrosion resistance and lower biocompatibility than compact alloy. This could affect health on long‐term exposure, especially in sensitized individuals.</description><subject>alloy</subject><subject>Aluminum - chemistry</subject><subject>Aluminum - toxicity</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Shape - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chromium - chemistry</subject><subject>Chromium - toxicity</subject><subject>Chromium Alloys - chemistry</subject><subject>Chromium Alloys - toxicity</subject><subject>Cobalt - chemistry</subject><subject>Cobalt - toxicity</subject><subject>Corrosion</subject><subject>cytotoxicity</subject><subject>Dental Casting Investment - chemistry</subject><subject>Dental Casting Investment - toxicity</subject><subject>Dentistry</subject><subject>Diamond - chemistry</subject><subject>Fibroblasts - drug effects</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Iron - chemistry</subject><subject>Iron - toxicity</subject><subject>Materials Testing</subject><subject>Mice</subject><subject>microparticles</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molybdenum - chemistry</subject><subject>Molybdenum - toxicity</subject><subject>Ni-Cr</subject><subject>Nickel - chemistry</subject><subject>Nickel - toxicity</subject><subject>Particle Size</subject><subject>Silicon - chemistry</subject><subject>Silicon - toxicity</subject><subject>Spectrophotometry, Atomic</subject><subject>Surface Properties</subject><issn>1059-941X</issn><issn>1532-849X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MtO3DAUBmALFZVbN32AKlI3FVLAJ4ljZ4lSYEDcOqXq7FwnOZE8TeJgZ2Dm7TGTgQULvLEX3_l1_BPyFegR-HM8N709gggo3SK7wOIoFEk2--TflGVhlsBsh-w5N6cUgAn4THaiBEBACrvkX26sNU4_YqC6KshXgxnMUpfBnTU92kGjC0wd5KbtVTkEv3ssdYudW-trXXqlvCqb0d3oMLfBT-wG1QQnTWNWB2S7Vo3DL5t7n_w5O73PJ-HV7flFfnIVlkkKNETgBTAUjIuIpxlgRaNCJDyqaFHUZSqyooqzOq5rzhLlNY15BFwkyHmRMoz3yY8xt7fmYYFukK12JTaN6tAsnATmNec0E55-f0fnZmE7v91axTxjnHl1OCr_R-cs1rK3ulV2JYHKl97lS-9y3bvH3zaRi6LF6o2-Fu0BjOBJN7j6IEpe3t5NX0PDcUa7AZdvM8r-l6nfksm_N-eSplM2-zWJ5Cx-BlrFm3s</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Ristic, Ljubisa</creator><creator>Vucevic, Dragana</creator><creator>Radovic, Ljubica</creator><creator>Djordjevic, Snezana</creator><creator>Nikacevic, Milutin</creator><creator>Colic, Miodrag</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Corrosive and Cytotoxic Properties of Compact Specimens and Microparticles of Ni-Cr Dental Alloy</title><author>Ristic, Ljubisa ; Vucevic, Dragana ; Radovic, Ljubica ; Djordjevic, Snezana ; Nikacevic, Milutin ; Colic, Miodrag</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4610-e17b15e857827691ed02b8472d0bbfc689bd39f3ff754ae1703721784e77b65e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>alloy</topic><topic>Aluminum - chemistry</topic><topic>Aluminum - toxicity</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Shape - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Chromium - chemistry</topic><topic>Chromium - toxicity</topic><topic>Chromium Alloys - chemistry</topic><topic>Chromium Alloys - toxicity</topic><topic>Cobalt - chemistry</topic><topic>Cobalt - toxicity</topic><topic>Corrosion</topic><topic>cytotoxicity</topic><topic>Dental Casting Investment - chemistry</topic><topic>Dental Casting Investment - toxicity</topic><topic>Dentistry</topic><topic>Diamond - chemistry</topic><topic>Fibroblasts - drug effects</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Iron - chemistry</topic><topic>Iron - toxicity</topic><topic>Materials Testing</topic><topic>Mice</topic><topic>microparticles</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molybdenum - chemistry</topic><topic>Molybdenum - toxicity</topic><topic>Ni-Cr</topic><topic>Nickel - chemistry</topic><topic>Nickel - toxicity</topic><topic>Particle Size</topic><topic>Silicon - chemistry</topic><topic>Silicon - toxicity</topic><topic>Spectrophotometry, Atomic</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ristic, Ljubisa</creatorcontrib><creatorcontrib>Vucevic, Dragana</creatorcontrib><creatorcontrib>Radovic, Ljubica</creatorcontrib><creatorcontrib>Djordjevic, Snezana</creatorcontrib><creatorcontrib>Nikacevic, Milutin</creatorcontrib><creatorcontrib>Colic, Miodrag</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of prosthodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ristic, Ljubisa</au><au>Vucevic, Dragana</au><au>Radovic, Ljubica</au><au>Djordjevic, Snezana</au><au>Nikacevic, Milutin</au><au>Colic, Miodrag</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corrosive and Cytotoxic Properties of Compact Specimens and Microparticles of Ni-Cr Dental Alloy</atitle><jtitle>Journal of prosthodontics</jtitle><addtitle>Journal of Prosthodontics</addtitle><date>2014-04</date><risdate>2014</risdate><volume>23</volume><issue>3</issue><spage>221</spage><epage>226</epage><pages>221-226</pages><issn>1059-941X</issn><eissn>1532-849X</eissn><abstract>Purpose
Nickel‐chromium (Ni‐Cr) dental alloys have been widely used in prosthodontic practice, but there is a permanent concern about their biocompatibility due to the release of metal ions. This is especially important when Ni‐Cr metal microparticles are incorporated into gingival tissue during prosthodontic procedures. Therefore, the aim of this study was to examine and compare the corrosion and cytotoxic properties of compact specimens and microparticles of Ni‐Cr dental alloy.
Materials and Methods
Ni‐Cr alloy, Remanium CSe bars (4 mm diameter), were made by the standard casting method and then cut into 0.5‐mm‐thick disks. Metal particles were obtained by scraping the bars using a diamond instrument for crown preparation. The microstructure was observed by an optical microscope. Quantitative determination and morphological and dimensional characterization of metal particles were carried out by a scanning electron microscope and Leica Application Suite software for image analysis. Corrosion was studied by conditioning the alloy specimens in the RPMI 1640 medium, containing 10% fetal calf serum in an incubator with 5% CO2 for 72 hours at 37°C. Inductively coupled plasma‐optical emission spectrometry was used to assess metal ion release. The cytotoxity of conditioning medium (CM) was investigated on L929 cells using an MTT test. One‐way ANOVA was used for statistical analysis.
Results
After casting, the microstructure of the Remanium CSe compact specimen composed of Ni, Cr, Mo, Si, Fe, Al, and Co had a typical dendritic structure. Alloy microparticles had an irregular shape with a wide size range: from less than 1 μm to more than 100 μm. The release of metal ions, especially Ni and Mo from microparticles, was significantly higher, compared to the compact alloy specimen. The CM prepared from compact alloy was not cytotoxic at any tested dilutions, whereas CM from alloy microparticles showed dose‐dependent cytotoxicity (90% CM and 45% CM versus control; p < 0.005).
Conclusion
Ni‐Cr microparticles showed less corrosion resistance and lower biocompatibility than compact alloy. This could affect health on long‐term exposure, especially in sensitized individuals.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24118161</pmid><doi>10.1111/jopr.12100</doi><tpages>6</tpages></addata></record> |
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| subjects | alloy Aluminum - chemistry Aluminum - toxicity Animals Cell Line Cell Shape - drug effects Cell Survival - drug effects Chromium - chemistry Chromium - toxicity Chromium Alloys - chemistry Chromium Alloys - toxicity Cobalt - chemistry Cobalt - toxicity Corrosion cytotoxicity Dental Casting Investment - chemistry Dental Casting Investment - toxicity Dentistry Diamond - chemistry Fibroblasts - drug effects Image Processing, Computer-Assisted - methods Iron - chemistry Iron - toxicity Materials Testing Mice microparticles Microscopy, Electron, Scanning Molybdenum - chemistry Molybdenum - toxicity Ni-Cr Nickel - chemistry Nickel - toxicity Particle Size Silicon - chemistry Silicon - toxicity Spectrophotometry, Atomic Surface Properties |
| title | Corrosive and Cytotoxic Properties of Compact Specimens and Microparticles of Ni-Cr Dental Alloy |
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