Succinic acid production from sucrose by Actinobacillus succinogenes NJ113
•A. succinogenes produced high concentration of succinic acid in high productivity.•Sucrose is transported and utilized via a sucrose PTS by A. succinogenes NJ113.•Succinate productivity increased by 35% via a fed-batch culture.•A. succinogenes NJ113 could tolerate more formic acid in medium with su...
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| Veröffentlicht in: | Bioresource technology 2014-02, Vol.153, p.327-332 |
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| creator | Jiang, Min Dai, Wenyu Xi, Yonglan Wu, Mingke Kong, Xiangping Ma, Jiangfeng Zhang, Min Chen, Kequan Wei, Ping |
| description | •A. succinogenes produced high concentration of succinic acid in high productivity.•Sucrose is transported and utilized via a sucrose PTS by A. succinogenes NJ113.•Succinate productivity increased by 35% via a fed-batch culture.•A. succinogenes NJ113 could tolerate more formic acid in medium with sucrose.•High succinic acid productivity made the process more economic.
In this study, sucrose, a reproducible disaccharide extracted from plants, was used as the carbon source for the production of succinic acid by Actinobacillus succinogenes NJ113. During serum bottle fermentation, the succinic acid concentration reached 57.1g/L with a yield of 71.5%. Further analysis of the sucrose utilization pathways revealed that sucrose was transported and utilized via a sucrose phosphotransferase system, sucrose-6-phosphate hydrolase, and a fructose PTS. Compared to glucose utilization in single pathway, more pathways of A. succinogenes NJ113 are dependent on sucrose utilization. By changing the control strategy in a fed-batch culture to alleviate sucrose inhibition, 60.5g/L of succinic acid was accumulated with a yield of 82.9%, and the productivity increased by 35.2%, reaching 2.16g/L/h. Thus utilization of sucrose has considerable potential economics and environmental meaning. |
| doi_str_mv | 10.1016/j.biortech.2013.11.062 |
| format | Article |
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In this study, sucrose, a reproducible disaccharide extracted from plants, was used as the carbon source for the production of succinic acid by Actinobacillus succinogenes NJ113. During serum bottle fermentation, the succinic acid concentration reached 57.1g/L with a yield of 71.5%. Further analysis of the sucrose utilization pathways revealed that sucrose was transported and utilized via a sucrose phosphotransferase system, sucrose-6-phosphate hydrolase, and a fructose PTS. Compared to glucose utilization in single pathway, more pathways of A. succinogenes NJ113 are dependent on sucrose utilization. By changing the control strategy in a fed-batch culture to alleviate sucrose inhibition, 60.5g/L of succinic acid was accumulated with a yield of 82.9%, and the productivity increased by 35.2%, reaching 2.16g/L/h. Thus utilization of sucrose has considerable potential economics and environmental meaning.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2013.11.062</identifier><identifier>PMID: 24393713</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>A. succinogenes NJ113 ; Actinobacillus - drug effects ; Actinobacillus - metabolism ; Batch Cell Culture Techniques ; Biological and medical sciences ; Biological Transport - drug effects ; Carbon ; Carbon - pharmacology ; Culture ; Fed-batch culture ; Fermentation - drug effects ; Fructose ; Fructose - metabolism ; Fundamental and applied biological sciences. Psychology ; Metabolic Networks and Pathways - drug effects ; Pathways ; Phosphotransferases - metabolism ; Strategy ; Succinic acid ; Succinic Acid - metabolism ; Sucrose ; Sucrose - metabolism ; Utilization ; Utilization pathways</subject><ispartof>Bioresource technology, 2014-02, Vol.153, p.327-332</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-ab830584886a2047336b8aeb6ee48d11933e6b8c6b9b06da2c6f5b363229ef8f3</citedby><cites>FETCH-LOGICAL-c431t-ab830584886a2047336b8aeb6ee48d11933e6b8c6b9b06da2c6f5b363229ef8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852413017884$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28282140$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24393713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Min</creatorcontrib><creatorcontrib>Dai, Wenyu</creatorcontrib><creatorcontrib>Xi, Yonglan</creatorcontrib><creatorcontrib>Wu, Mingke</creatorcontrib><creatorcontrib>Kong, Xiangping</creatorcontrib><creatorcontrib>Ma, Jiangfeng</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Chen, Kequan</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><title>Succinic acid production from sucrose by Actinobacillus succinogenes NJ113</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•A. succinogenes produced high concentration of succinic acid in high productivity.•Sucrose is transported and utilized via a sucrose PTS by A. succinogenes NJ113.•Succinate productivity increased by 35% via a fed-batch culture.•A. succinogenes NJ113 could tolerate more formic acid in medium with sucrose.•High succinic acid productivity made the process more economic.
In this study, sucrose, a reproducible disaccharide extracted from plants, was used as the carbon source for the production of succinic acid by Actinobacillus succinogenes NJ113. During serum bottle fermentation, the succinic acid concentration reached 57.1g/L with a yield of 71.5%. Further analysis of the sucrose utilization pathways revealed that sucrose was transported and utilized via a sucrose phosphotransferase system, sucrose-6-phosphate hydrolase, and a fructose PTS. Compared to glucose utilization in single pathway, more pathways of A. succinogenes NJ113 are dependent on sucrose utilization. By changing the control strategy in a fed-batch culture to alleviate sucrose inhibition, 60.5g/L of succinic acid was accumulated with a yield of 82.9%, and the productivity increased by 35.2%, reaching 2.16g/L/h. Thus utilization of sucrose has considerable potential economics and environmental meaning.</description><subject>A. succinogenes NJ113</subject><subject>Actinobacillus - drug effects</subject><subject>Actinobacillus - metabolism</subject><subject>Batch Cell Culture Techniques</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Carbon</subject><subject>Carbon - pharmacology</subject><subject>Culture</subject><subject>Fed-batch culture</subject><subject>Fermentation - drug effects</subject><subject>Fructose</subject><subject>Fructose - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolic Networks and Pathways - drug effects</subject><subject>Pathways</subject><subject>Phosphotransferases - metabolism</subject><subject>Strategy</subject><subject>Succinic acid</subject><subject>Succinic Acid - metabolism</subject><subject>Sucrose</subject><subject>Sucrose - metabolism</subject><subject>Utilization</subject><subject>Utilization pathways</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EokvbV6hyQeKS4PE4jnOjqmhpVcEBOFu2MwGvsnGxk0p9e7zaLRwrHyzNfL9n_DF2AbwBDurjtnEhpoX870ZwwAag4Uq8YhvQHdai79RrtuG94rVuhTxh73Lecs4ROvGWnQiJPXaAG3b3ffU-zMFX1oehekhxWP0S4lyNKe6qvPoUM1Xuqbos5Tm6gk3Tmvedkou_aKZcfb0DwDP2ZrRTpvPjfcp-Xn_-cfWlvv92c3t1eV97ibDU1mnkrZZaKyu47BCV05acIpJ6AOgRqVS8cr3jarDCq7F1qFCInkY94in7cHi3LPtnpbyYXciepsnOFNdsoAXVtQr77mVU9rwrI6UsqDqg-w_nRKN5SGFn05MBbvbKzdY8Kzd75QbAFOUleHGcsbodDf9iz44L8P4I2OztNCY7-5D_c7ockLxwnw4cFXmPgZLJPtDsaQiJ_GKGGF7a5S_TC6Ft</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Jiang, Min</creator><creator>Dai, Wenyu</creator><creator>Xi, Yonglan</creator><creator>Wu, Mingke</creator><creator>Kong, Xiangping</creator><creator>Ma, Jiangfeng</creator><creator>Zhang, Min</creator><creator>Chen, Kequan</creator><creator>Wei, Ping</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20140201</creationdate><title>Succinic acid production from sucrose by Actinobacillus succinogenes NJ113</title><author>Jiang, Min ; Dai, Wenyu ; Xi, Yonglan ; Wu, Mingke ; Kong, Xiangping ; Ma, Jiangfeng ; Zhang, Min ; Chen, Kequan ; Wei, Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-ab830584886a2047336b8aeb6ee48d11933e6b8c6b9b06da2c6f5b363229ef8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>A. succinogenes NJ113</topic><topic>Actinobacillus - drug effects</topic><topic>Actinobacillus - metabolism</topic><topic>Batch Cell Culture Techniques</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Carbon</topic><topic>Carbon - pharmacology</topic><topic>Culture</topic><topic>Fed-batch culture</topic><topic>Fermentation - drug effects</topic><topic>Fructose</topic><topic>Fructose - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolic Networks and Pathways - drug effects</topic><topic>Pathways</topic><topic>Phosphotransferases - metabolism</topic><topic>Strategy</topic><topic>Succinic acid</topic><topic>Succinic Acid - metabolism</topic><topic>Sucrose</topic><topic>Sucrose - metabolism</topic><topic>Utilization</topic><topic>Utilization pathways</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Min</creatorcontrib><creatorcontrib>Dai, Wenyu</creatorcontrib><creatorcontrib>Xi, Yonglan</creatorcontrib><creatorcontrib>Wu, Mingke</creatorcontrib><creatorcontrib>Kong, Xiangping</creatorcontrib><creatorcontrib>Ma, Jiangfeng</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Chen, Kequan</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Min</au><au>Dai, Wenyu</au><au>Xi, Yonglan</au><au>Wu, Mingke</au><au>Kong, Xiangping</au><au>Ma, Jiangfeng</au><au>Zhang, Min</au><au>Chen, Kequan</au><au>Wei, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Succinic acid production from sucrose by Actinobacillus succinogenes NJ113</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>153</volume><spage>327</spage><epage>332</epage><pages>327-332</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>•A. succinogenes produced high concentration of succinic acid in high productivity.•Sucrose is transported and utilized via a sucrose PTS by A. succinogenes NJ113.•Succinate productivity increased by 35% via a fed-batch culture.•A. succinogenes NJ113 could tolerate more formic acid in medium with sucrose.•High succinic acid productivity made the process more economic.
In this study, sucrose, a reproducible disaccharide extracted from plants, was used as the carbon source for the production of succinic acid by Actinobacillus succinogenes NJ113. During serum bottle fermentation, the succinic acid concentration reached 57.1g/L with a yield of 71.5%. Further analysis of the sucrose utilization pathways revealed that sucrose was transported and utilized via a sucrose phosphotransferase system, sucrose-6-phosphate hydrolase, and a fructose PTS. Compared to glucose utilization in single pathway, more pathways of A. succinogenes NJ113 are dependent on sucrose utilization. By changing the control strategy in a fed-batch culture to alleviate sucrose inhibition, 60.5g/L of succinic acid was accumulated with a yield of 82.9%, and the productivity increased by 35.2%, reaching 2.16g/L/h. Thus utilization of sucrose has considerable potential economics and environmental meaning.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24393713</pmid><doi>10.1016/j.biortech.2013.11.062</doi><tpages>6</tpages></addata></record> |
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| subjects | A. succinogenes NJ113 Actinobacillus - drug effects Actinobacillus - metabolism Batch Cell Culture Techniques Biological and medical sciences Biological Transport - drug effects Carbon Carbon - pharmacology Culture Fed-batch culture Fermentation - drug effects Fructose Fructose - metabolism Fundamental and applied biological sciences. Psychology Metabolic Networks and Pathways - drug effects Pathways Phosphotransferases - metabolism Strategy Succinic acid Succinic Acid - metabolism Sucrose Sucrose - metabolism Utilization Utilization pathways |
| title | Succinic acid production from sucrose by Actinobacillus succinogenes NJ113 |
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