Novel strategy for production of aggregation-prone proteins and lytic enzymes in Escherichia coli based on an anchored periplasmic expression system

For over 2 decades, Escherichia coli has been successfully used for the production of various recombinant proteins. However, several technical limitations have influenced the extent of recombinant protein expression in the E. coli host because of (i) heterologous protein accumulation often observed...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2013-11, Vol.116 (5), p.638-643
Hauptverfasser:	Lee, Jae Hyung, Velmurugan, Natarajan, Jeong, Ki Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Anchored periplasmic expression Biocatalysis Biological and medical sciences Biotechnology Cell Membrane - metabolism Escherichia coli Escherichia coli - cytology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Humans Immobilized Proteins - biosynthesis Immobilized Proteins - genetics Immobilized Proteins - isolation & purification Immobilized Proteins - metabolism Inclusion Bodies - metabolism Leptin Leptin - biosynthesis Leptin - genetics Leptin - isolation & purification Lipase Lipase - biosynthesis Lipase - genetics Lipase - isolation & purification Microbial Viability Periplasm Periplasm - metabolism Pseudomonas fluorescens Pseudomonas fluorescens - enzymology Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification
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