Human cell growth regulator Ly‐1 antibody reactive homologue accelerates processing of preribosomal RNA
Ribosome biogenesis is an essential process for cell growth and proliferation and is enhanced in cancer and embryonic stem cells. Mouse Ly‐1 antibody reactive clone product (Lyar) is expressed at very high levels in many tumor, leukemia or embryonic stem cells; is a novel nucleolar protein with zinc...
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| Veröffentlicht in: | Genes to cells : devoted to molecular & cellular mechanisms 2014-04, Vol.19 (4), p.273-286 |
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| creator | Miyazawa, Naoki Yoshikawa, Harunori Magae, Satomi Ishikawa, Hideaki Izumikawa, Keiichi Terukina, Goro Suzuki, Ai Nakamura‐Fujiyama, Sally Miura, Yutaka Hayano, Toshiya Komatsu, Wataru Isobe, Toshiaki Takahashi, Nobuhiro |
| description | Ribosome biogenesis is an essential process for cell growth and proliferation and is enhanced in cancer and embryonic stem cells. Mouse Ly‐1 antibody reactive clone product (Lyar) is expressed at very high levels in many tumor, leukemia or embryonic stem cells; is a novel nucleolar protein with zinc‐finger DNA‐binding motifs and is involved in cell growth regulation. However, cellular function of Lyar remains unexplored. Here, we show that human homologue of Lyar (LYAR) accelerates ribosome biogenesis at the level of processing of preribosomal RNA (pre‐rRNA). We show that LYAR is excluded from the nucleolus after actinomycin D treatment and is present in preribosomal fraction of the nuclear extract as well as in the fractions with 40S, 60S and 90S sedimentation coefficients. LYAR is required for processing of 47S/45S, 32S, 30S and 21S pre‐rRNAs. In addition, we show that over‐expression of LYAR increases cell proliferation without affecting the expression of c‐Myc or p53. Combined, these results suggest that some rapidly growing cells enhance ribosome biogenesis by increasing the expression of LYAR. |
| doi_str_mv | 10.1111/gtc.12129 |
| format | Article |
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Mouse Ly‐1 antibody reactive clone product (Lyar) is expressed at very high levels in many tumor, leukemia or embryonic stem cells; is a novel nucleolar protein with zinc‐finger DNA‐binding motifs and is involved in cell growth regulation. However, cellular function of Lyar remains unexplored. Here, we show that human homologue of Lyar (LYAR) accelerates ribosome biogenesis at the level of processing of preribosomal RNA (pre‐rRNA). We show that LYAR is excluded from the nucleolus after actinomycin D treatment and is present in preribosomal fraction of the nuclear extract as well as in the fractions with 40S, 60S and 90S sedimentation coefficients. LYAR is required for processing of 47S/45S, 32S, 30S and 21S pre‐rRNAs. In addition, we show that over‐expression of LYAR increases cell proliferation without affecting the expression of c‐Myc or p53. Combined, these results suggest that some rapidly growing cells enhance ribosome biogenesis by increasing the expression of LYAR.</description><identifier>ISSN: 1356-9597</identifier><identifier>EISSN: 1365-2443</identifier><identifier>DOI: 10.1111/gtc.12129</identifier><identifier>PMID: 24495227</identifier><identifier>CODEN: GECEFL</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Biosynthesis ; Cell growth ; Cell Proliferation ; Dactinomycin - pharmacology ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; HEK293 Cells ; HeLa Cells ; Humans ; Mice ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Nucleic Acid Synthesis Inhibitors - pharmacology ; RNA Precursors - metabolism ; RNA Processing, Post-Transcriptional ; RNA, Ribosomal - metabolism ; Stem cells ; Structural Homology, Protein</subject><ispartof>Genes to cells : devoted to molecular & cellular mechanisms, 2014-04, Vol.19 (4), p.273-286</ispartof><rights>2014 The Authors Genes to Cells © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd</rights><rights>2014 The Authors Genes to Cells © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.</rights><rights>Genes to Cells © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4529-960047ed4478e5b12c7f4d95c4bfc8f48592ba376248e330ad814869f45665933</citedby><cites>FETCH-LOGICAL-c4529-960047ed4478e5b12c7f4d95c4bfc8f48592ba376248e330ad814869f45665933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fgtc.12129$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fgtc.12129$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24495227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyazawa, Naoki</creatorcontrib><creatorcontrib>Yoshikawa, Harunori</creatorcontrib><creatorcontrib>Magae, Satomi</creatorcontrib><creatorcontrib>Ishikawa, Hideaki</creatorcontrib><creatorcontrib>Izumikawa, Keiichi</creatorcontrib><creatorcontrib>Terukina, Goro</creatorcontrib><creatorcontrib>Suzuki, Ai</creatorcontrib><creatorcontrib>Nakamura‐Fujiyama, Sally</creatorcontrib><creatorcontrib>Miura, Yutaka</creatorcontrib><creatorcontrib>Hayano, Toshiya</creatorcontrib><creatorcontrib>Komatsu, Wataru</creatorcontrib><creatorcontrib>Isobe, Toshiaki</creatorcontrib><creatorcontrib>Takahashi, Nobuhiro</creatorcontrib><title>Human cell growth regulator Ly‐1 antibody reactive homologue accelerates processing of preribosomal RNA</title><title>Genes to cells : devoted to molecular & cellular mechanisms</title><addtitle>Genes Cells</addtitle><description>Ribosome biogenesis is an essential process for cell growth and proliferation and is enhanced in cancer and embryonic stem cells. Mouse Ly‐1 antibody reactive clone product (Lyar) is expressed at very high levels in many tumor, leukemia or embryonic stem cells; is a novel nucleolar protein with zinc‐finger DNA‐binding motifs and is involved in cell growth regulation. However, cellular function of Lyar remains unexplored. Here, we show that human homologue of Lyar (LYAR) accelerates ribosome biogenesis at the level of processing of preribosomal RNA (pre‐rRNA). We show that LYAR is excluded from the nucleolus after actinomycin D treatment and is present in preribosomal fraction of the nuclear extract as well as in the fractions with 40S, 60S and 90S sedimentation coefficients. LYAR is required for processing of 47S/45S, 32S, 30S and 21S pre‐rRNAs. In addition, we show that over‐expression of LYAR increases cell proliferation without affecting the expression of c‐Myc or p53. Combined, these results suggest that some rapidly growing cells enhance ribosome biogenesis by increasing the expression of LYAR.</description><subject>Animals</subject><subject>Biosynthesis</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Dactinomycin - pharmacology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>HEK293 Cells</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Mice</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nucleic Acid Synthesis Inhibitors - pharmacology</subject><subject>RNA Precursors - metabolism</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA, Ribosomal - metabolism</subject><subject>Stem cells</subject><subject>Structural Homology, Protein</subject><issn>1356-9597</issn><issn>1365-2443</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1KxDAUhYMo_owufAEJuNFFNb9ts5RBZ4RBQXRd0vS2VtpGk9Zhdj6Cz-iTmHFGF4JgNsnlfvfcHA5Ch5Sc0XDOq96cUUaZ2kC7lMcyYkLwzeVbxpGSKtlBe94_EUI5I3Ib7YS-kowlu6ieDq3usIGmwZWz8_4RO6iGRvfW4dni4-2dYt31dW6LReho09evgB9taxtbDYC1CaPgdA8ePztrwPu6q7AtQwUujHnb6gbf3Vzso61SNx4O1vcIPVxd3o-n0ex2cj2-mEVGSKYiFRMiEiiESFKQOWUmKUWhpBF5adJSpFKxXPMkZiIFzokuUirSWJVCxrFUnI_QyUo3fOdlAN9nbe2X_nQHdvAZlTROJKdK_gclgnCVJgE9_oU-2cF1wUigiJI0FXy5-3RFGWe9d1Bmz65utVtklGTLqLIQVfYVVWCP1opD3kLxQ35nE4DzFTCvG1j8rZRN7scryU8cTpzK</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Miyazawa, Naoki</creator><creator>Yoshikawa, Harunori</creator><creator>Magae, Satomi</creator><creator>Ishikawa, Hideaki</creator><creator>Izumikawa, Keiichi</creator><creator>Terukina, Goro</creator><creator>Suzuki, Ai</creator><creator>Nakamura‐Fujiyama, Sally</creator><creator>Miura, Yutaka</creator><creator>Hayano, Toshiya</creator><creator>Komatsu, Wataru</creator><creator>Isobe, Toshiaki</creator><creator>Takahashi, Nobuhiro</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Human cell growth regulator Ly‐1 antibody reactive homologue accelerates processing of preribosomal RNA</title><author>Miyazawa, Naoki ; Yoshikawa, Harunori ; Magae, Satomi ; Ishikawa, Hideaki ; Izumikawa, Keiichi ; Terukina, Goro ; Suzuki, Ai ; Nakamura‐Fujiyama, Sally ; Miura, Yutaka ; Hayano, Toshiya ; Komatsu, Wataru ; Isobe, Toshiaki ; Takahashi, Nobuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4529-960047ed4478e5b12c7f4d95c4bfc8f48592ba376248e330ad814869f45665933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biosynthesis</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Dactinomycin - pharmacology</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>HEK293 Cells</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Mice</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nucleic Acid Synthesis Inhibitors - pharmacology</topic><topic>RNA Precursors - metabolism</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA, Ribosomal - metabolism</topic><topic>Stem cells</topic><topic>Structural Homology, Protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyazawa, Naoki</creatorcontrib><creatorcontrib>Yoshikawa, Harunori</creatorcontrib><creatorcontrib>Magae, Satomi</creatorcontrib><creatorcontrib>Ishikawa, Hideaki</creatorcontrib><creatorcontrib>Izumikawa, Keiichi</creatorcontrib><creatorcontrib>Terukina, Goro</creatorcontrib><creatorcontrib>Suzuki, Ai</creatorcontrib><creatorcontrib>Nakamura‐Fujiyama, Sally</creatorcontrib><creatorcontrib>Miura, Yutaka</creatorcontrib><creatorcontrib>Hayano, Toshiya</creatorcontrib><creatorcontrib>Komatsu, Wataru</creatorcontrib><creatorcontrib>Isobe, Toshiaki</creatorcontrib><creatorcontrib>Takahashi, Nobuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes to cells : devoted to molecular & cellular mechanisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyazawa, Naoki</au><au>Yoshikawa, Harunori</au><au>Magae, Satomi</au><au>Ishikawa, Hideaki</au><au>Izumikawa, Keiichi</au><au>Terukina, Goro</au><au>Suzuki, Ai</au><au>Nakamura‐Fujiyama, Sally</au><au>Miura, Yutaka</au><au>Hayano, Toshiya</au><au>Komatsu, Wataru</au><au>Isobe, Toshiaki</au><au>Takahashi, Nobuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human cell growth regulator Ly‐1 antibody reactive homologue accelerates processing of preribosomal RNA</atitle><jtitle>Genes to cells : devoted to molecular & cellular mechanisms</jtitle><addtitle>Genes Cells</addtitle><date>2014-04</date><risdate>2014</risdate><volume>19</volume><issue>4</issue><spage>273</spage><epage>286</epage><pages>273-286</pages><issn>1356-9597</issn><eissn>1365-2443</eissn><coden>GECEFL</coden><abstract>Ribosome biogenesis is an essential process for cell growth and proliferation and is enhanced in cancer and embryonic stem cells. Mouse Ly‐1 antibody reactive clone product (Lyar) is expressed at very high levels in many tumor, leukemia or embryonic stem cells; is a novel nucleolar protein with zinc‐finger DNA‐binding motifs and is involved in cell growth regulation. However, cellular function of Lyar remains unexplored. Here, we show that human homologue of Lyar (LYAR) accelerates ribosome biogenesis at the level of processing of preribosomal RNA (pre‐rRNA). We show that LYAR is excluded from the nucleolus after actinomycin D treatment and is present in preribosomal fraction of the nuclear extract as well as in the fractions with 40S, 60S and 90S sedimentation coefficients. LYAR is required for processing of 47S/45S, 32S, 30S and 21S pre‐rRNAs. In addition, we show that over‐expression of LYAR increases cell proliferation without affecting the expression of c‐Myc or p53. Combined, these results suggest that some rapidly growing cells enhance ribosome biogenesis by increasing the expression of LYAR.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>24495227</pmid><doi>10.1111/gtc.12129</doi><tpages>14</tpages></addata></record> |
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| ispartof | Genes to cells : devoted to molecular & cellular mechanisms, 2014-04, Vol.19 (4), p.273-286 |
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| subjects | Animals Biosynthesis Cell growth Cell Proliferation Dactinomycin - pharmacology DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism HEK293 Cells HeLa Cells Humans Mice Nuclear Proteins - genetics Nuclear Proteins - metabolism Nucleic Acid Synthesis Inhibitors - pharmacology RNA Precursors - metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal - metabolism Stem cells Structural Homology, Protein |
| title | Human cell growth regulator Ly‐1 antibody reactive homologue accelerates processing of preribosomal RNA |
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