Cell surface display of organophosphorus hydrolase for sensitive spectrophotometric detection of p-nitrophenol substituted organophosphates

•OPH displayed on the surface of Escherichia coli using INP display system.•OPH whole cell biocatalyst showed excellent enzyme activity and stability.•OPH strain capable of detecting PNP substituted OPs sensitively. Organophosphates (OPs) widely exist in ecosystem as toxic substances, for which sens...

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Veröffentlicht in:	Enzyme and microbial technology 2014-02, Vol.55, p.107-112
Hauptverfasser:	Tang, Xiangjiang, Liang, Bo, Yi, Tuyong, Manco, Giuseppe, IlariaPalchetti, Liu, Aihua
Format:	Artikel
Sprache:	eng
Schlagworte:	Aryldialkylphosphatase - genetics Aryldialkylphosphatase - metabolism Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - metabolism Bacterial surface display Biocatalysis Calibration Carrier Proteins - genetics Carrier Proteins - metabolism Cell Membrane - chemistry Environmental Pollutants - analysis Escherichia coli Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fenitrothion - analysis Fenitrothion - metabolism Genes, Synthetic Glycosylphosphatidylinositols - genetics Hydrogen-Ion Concentration Ice nucleation protein Methyl Parathion - analysis Methyl Parathion - metabolism Molecular Structure Nitrophenols - analysis Organophosphates - analysis Organophosphorus hydrolase p-Nitrophenol Paraoxon - analysis Paraoxon - metabolism Parathion - analysis Parathion - metabolism Pesticide Residues - analysis Recombinant Fusion Proteins - metabolism Spectrophotometric detection of organophosphates Spectrophotometry - methods Temperature
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