Characterization of a trifunctional fatty acid desaturase from oleaginous filamentous fungus Mortierella alpina 1S-4 using a yeast expression system

A ω3-fatty acid desaturase gene (maw3) which is involved in biosynthesis of n-3 polyunsaturated fatty acids (PUFAs) was previously isolated from Mortierella alpina 1S-4. In this report, we investigated the products of MAW3 catalyzing reaction with endogenous and exogenous fatty acids in the yeast tr...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2013-12, Vol.116 (6), p.672-676
Hauptverfasser:	Kikukawa, Hiroshi, Sakuradani, Eiji, Kishino, Shigenobu, Park, Si-Bum, Ando, Akinori, Shima, Jun, Ochiai, Misa, Shimizu, Sakayu, Ogawa, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences biosynthesis Biotechnology Fatty Acid Desaturases - genetics Fatty Acid Desaturases - metabolism Fatty Acids, Monounsaturated - metabolism Fatty Acids, Unsaturated - biosynthesis Fundamental and applied biological sciences. Psychology gas chromatography-mass spectrometry Gene Expression genes Hexadecadienoic acid Hexadecatrienoic acid Mortierella - enzymology Mortierella - genetics Mortierella alpina nuclear magnetic resonance spectroscopy Nuclear Magnetic Resonance, Biomolecular omega-3 fatty acids palmitoleic acid Polyunsaturated fatty acid polyunsaturated fatty acids Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism stearoyl-CoA desaturase temperature Trifunctional fatty acid desaturase yeasts
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