The potential interaction of MARCKS-related peptide and diltiazem on acrolin-induced airway mucus hypersecretion in rats
Airway mucus hypersecretion is recognized as a pathophysiological feature of airway inflammation. Ca2+ entry and myristoylated alanine-rich C kinase substrate translocation are considered as important factors in such process. To investigate the potential interaction of myristoylated alanine-rich C k...
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| Veröffentlicht in: | International immunopharmacology 2013-11, Vol.17 (3), p.625-632 |
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| creator | Chen, Peng Deng, Zhiping Wang, Tao Chen, Lei Li, Jiqiong Feng, Yulin Zhang, Shangfu Nin, Yunyie Liu, Daishun Chen, Yajuan Ou, Xuemei Wen, Fuqiang |
| description | Airway mucus hypersecretion is recognized as a pathophysiological feature of airway inflammation. Ca2+ entry and myristoylated alanine-rich C kinase substrate translocation are considered as important factors in such process. To investigate the potential interaction of myristoylated alanine-rich C kinase substrate (MARCKS)-related peptide and diltiazem on acrolein-induced airway mucus hypersecretion in rats, rat model of airway mucus hypersecretion was established by inhalation of acrolein on 12 consecutive days. MARCKS-related peptide, diltiazem, saline or the combination (MARCKS-related peptide+diltiazem) was intratracheally administered respectively. The rats were received pilocarpine to stimulate mucus release before sacrifices. The expression of Mucin5ac in bronchoalveolar lavage fluid (BALF) was measured by ELISA. Intracellular Muc5ac level was detected by immunohistochemical staining and western-blot. Muc5ac mRNA in lung was analyzed by RT-PCR. Results: Instillation of MARCKS-related peptide attenuated the release of Muc5ac in BALF induced by acrolein(p |
| doi_str_mv | 10.1016/j.intimp.2013.08.001 |
| format | Article |
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•We established the model of airway mucus hypersecretion induced by acrolein.•We found the interaction of MARCKS-related peptide and diltiazem on mucus secretion.•MARCKS-related peptide attenuated secretion and the effect was enhanced by diltiazem.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2013.08.001</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Airway ; Diltiazem ; MARCKS ; Mucus hypersecretion</subject><ispartof>International immunopharmacology, 2013-11, Vol.17 (3), p.625-632</ispartof><rights>2013 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-7ea1b6bbacf597e7f0cf366c4a21a30d4c2c214a81602f31c636c7768be1ec253</citedby><cites>FETCH-LOGICAL-c339t-7ea1b6bbacf597e7f0cf366c4a21a30d4c2c214a81602f31c636c7768be1ec253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567576913003135$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65308</link.rule.ids></links><search><creatorcontrib>Chen, Peng</creatorcontrib><creatorcontrib>Deng, Zhiping</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Li, Jiqiong</creatorcontrib><creatorcontrib>Feng, Yulin</creatorcontrib><creatorcontrib>Zhang, Shangfu</creatorcontrib><creatorcontrib>Nin, Yunyie</creatorcontrib><creatorcontrib>Liu, Daishun</creatorcontrib><creatorcontrib>Chen, Yajuan</creatorcontrib><creatorcontrib>Ou, Xuemei</creatorcontrib><creatorcontrib>Wen, Fuqiang</creatorcontrib><title>The potential interaction of MARCKS-related peptide and diltiazem on acrolin-induced airway mucus hypersecretion in rats</title><title>International immunopharmacology</title><description>Airway mucus hypersecretion is recognized as a pathophysiological feature of airway inflammation. Ca2+ entry and myristoylated alanine-rich C kinase substrate translocation are considered as important factors in such process. To investigate the potential interaction of myristoylated alanine-rich C kinase substrate (MARCKS)-related peptide and diltiazem on acrolein-induced airway mucus hypersecretion in rats, rat model of airway mucus hypersecretion was established by inhalation of acrolein on 12 consecutive days. MARCKS-related peptide, diltiazem, saline or the combination (MARCKS-related peptide+diltiazem) was intratracheally administered respectively. The rats were received pilocarpine to stimulate mucus release before sacrifices. The expression of Mucin5ac in bronchoalveolar lavage fluid (BALF) was measured by ELISA. Intracellular Muc5ac level was detected by immunohistochemical staining and western-blot. Muc5ac mRNA in lung was analyzed by RT-PCR. Results: Instillation of MARCKS-related peptide attenuated the release of Muc5ac in BALF induced by acrolein(p<0.05). Diltiazem alone had no effect on mucus hypersecretion induced by acrolein. However, the release of Muc5ac in BALF was further reduced when challenged with simultaneous instillation with MARCKS-related peptide and diltiazem, compared with MARCKS-related peptide alone (p<0.05). The intracellular level of Muc5ac in lung was increased when treated with MARCKS-related peptide alone or MARCKS-related peptide plus diltiazem (p<0.05). Nevertheless, diltiazem alone did not take effect as above. Conclusions: In the model of airway mucus hypersecretion induced by acrolein, MARCKS-related peptide attenuated mucus secretion and the inhibitory effect was enhanced by diltiazem, which may be due to a further diminution of the intracellular free calcium concentration and retention of mucin within epithelial goblet cells.
•We established the model of airway mucus hypersecretion induced by acrolein.•We found the interaction of MARCKS-related peptide and diltiazem on mucus secretion.•MARCKS-related peptide attenuated secretion and the effect was enhanced by diltiazem.</description><subject>Airway</subject><subject>Diltiazem</subject><subject>MARCKS</subject><subject>Mucus hypersecretion</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kDtPwzAURiMEEqXwDxg8siT45mGnC1JV8RIgJCiz5d7cqK7ywnaA8utxKTOTPZzvSPdE0TnwBDiIy01iOm_aIUk5ZAkvE87hIJpAKcsYJC8Ow78QMi6kmB1HJ85tAiB5DpPoa7kmNvSegkA3LHjIavSm71hfs6f5y-LhNbbUaE8VG2jwpiKmu4pVpgmLb2pZQDXavjFdbLpqxABqYz_1lrUjjo6ttwNZR2jpV2s6ZrV3p9FRrRtHZ3_vNHq7uV4u7uLH59v7xfwxxiyb-ViShpVYrTTWxUySrDnWmRCY6xR0xqscU0wh1yUIntYZoMgESinKFQFhWmTT6GLvHWz_PpLzqjUOqWl0R_3oFBQgZC54XgY036PhGucs1WqwptV2q4CrXWi1UfvQahda8VKFjmF2tZ9ROOPDkFUODXWhg7GEXlW9-V_wA-8Vi0A</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Chen, Peng</creator><creator>Deng, Zhiping</creator><creator>Wang, Tao</creator><creator>Chen, Lei</creator><creator>Li, Jiqiong</creator><creator>Feng, Yulin</creator><creator>Zhang, Shangfu</creator><creator>Nin, Yunyie</creator><creator>Liu, Daishun</creator><creator>Chen, Yajuan</creator><creator>Ou, Xuemei</creator><creator>Wen, Fuqiang</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20131101</creationdate><title>The potential interaction of MARCKS-related peptide and diltiazem on acrolin-induced airway mucus hypersecretion in rats</title><author>Chen, Peng ; Deng, Zhiping ; Wang, Tao ; Chen, Lei ; Li, Jiqiong ; Feng, Yulin ; Zhang, Shangfu ; Nin, Yunyie ; Liu, Daishun ; Chen, Yajuan ; Ou, Xuemei ; Wen, Fuqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-7ea1b6bbacf597e7f0cf366c4a21a30d4c2c214a81602f31c636c7768be1ec253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Airway</topic><topic>Diltiazem</topic><topic>MARCKS</topic><topic>Mucus hypersecretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Peng</creatorcontrib><creatorcontrib>Deng, Zhiping</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Li, Jiqiong</creatorcontrib><creatorcontrib>Feng, Yulin</creatorcontrib><creatorcontrib>Zhang, Shangfu</creatorcontrib><creatorcontrib>Nin, Yunyie</creatorcontrib><creatorcontrib>Liu, Daishun</creatorcontrib><creatorcontrib>Chen, Yajuan</creatorcontrib><creatorcontrib>Ou, Xuemei</creatorcontrib><creatorcontrib>Wen, Fuqiang</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Peng</au><au>Deng, Zhiping</au><au>Wang, Tao</au><au>Chen, Lei</au><au>Li, Jiqiong</au><au>Feng, Yulin</au><au>Zhang, Shangfu</au><au>Nin, Yunyie</au><au>Liu, Daishun</au><au>Chen, Yajuan</au><au>Ou, Xuemei</au><au>Wen, Fuqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The potential interaction of MARCKS-related peptide and diltiazem on acrolin-induced airway mucus hypersecretion in rats</atitle><jtitle>International immunopharmacology</jtitle><date>2013-11-01</date><risdate>2013</risdate><volume>17</volume><issue>3</issue><spage>625</spage><epage>632</epage><pages>625-632</pages><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>Airway mucus hypersecretion is recognized as a pathophysiological feature of airway inflammation. Ca2+ entry and myristoylated alanine-rich C kinase substrate translocation are considered as important factors in such process. To investigate the potential interaction of myristoylated alanine-rich C kinase substrate (MARCKS)-related peptide and diltiazem on acrolein-induced airway mucus hypersecretion in rats, rat model of airway mucus hypersecretion was established by inhalation of acrolein on 12 consecutive days. MARCKS-related peptide, diltiazem, saline or the combination (MARCKS-related peptide+diltiazem) was intratracheally administered respectively. The rats were received pilocarpine to stimulate mucus release before sacrifices. The expression of Mucin5ac in bronchoalveolar lavage fluid (BALF) was measured by ELISA. Intracellular Muc5ac level was detected by immunohistochemical staining and western-blot. Muc5ac mRNA in lung was analyzed by RT-PCR. Results: Instillation of MARCKS-related peptide attenuated the release of Muc5ac in BALF induced by acrolein(p<0.05). Diltiazem alone had no effect on mucus hypersecretion induced by acrolein. However, the release of Muc5ac in BALF was further reduced when challenged with simultaneous instillation with MARCKS-related peptide and diltiazem, compared with MARCKS-related peptide alone (p<0.05). The intracellular level of Muc5ac in lung was increased when treated with MARCKS-related peptide alone or MARCKS-related peptide plus diltiazem (p<0.05). Nevertheless, diltiazem alone did not take effect as above. Conclusions: In the model of airway mucus hypersecretion induced by acrolein, MARCKS-related peptide attenuated mucus secretion and the inhibitory effect was enhanced by diltiazem, which may be due to a further diminution of the intracellular free calcium concentration and retention of mucin within epithelial goblet cells.
•We established the model of airway mucus hypersecretion induced by acrolein.•We found the interaction of MARCKS-related peptide and diltiazem on mucus secretion.•MARCKS-related peptide attenuated secretion and the effect was enhanced by diltiazem.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.intimp.2013.08.001</doi><tpages>8</tpages></addata></record> |
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| subjects | Airway Diltiazem MARCKS Mucus hypersecretion |
| title | The potential interaction of MARCKS-related peptide and diltiazem on acrolin-induced airway mucus hypersecretion in rats |
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