Magnetic Nanoparticles Supported Ionic Liquids Improve Firefly Luciferase Properties
Ionic liquids as neoteric solvents, microwave irradiation, and alternative energy source are becoming as a solvent for many enzymatic reactions. We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of lucif...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2014-03, Vol.172 (6), p.3116-3127 |
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| creator | Noori, Ali Reza Hosseinkhani, Saman Ghiasi, Parisa Akbari, Jafar Heydari, Akbar |
| description | Ionic liquids as neoteric solvents, microwave irradiation, and alternative energy source are becoming as a solvent for many enzymatic reactions. We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of luciferase. Magnetic nanoparticles supported ionic liquids have been obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. In the present study, the effects of [γ-Fe₂O₃@SiO₂][BMImCl] and [γ-Fe₂O₃@SiO₂][BMImI] were investigated on the structural properties and function of luciferase using circular dichroism, fluorescence spectroscopy, and bioluminescence assay. Enzyme activity and structural stability increased in the presence of magnetic nanoparticles supported ionic liquids. Furthermore, the effect of ingredients which were used was not considerable on K ₘ value of luciferase for adenosine-5′-triphosphate and also K ₘ value for luciferin. |
| doi_str_mv | 10.1007/s12010-014-0730-8 |
| format | Article |
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We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of luciferase. Magnetic nanoparticles supported ionic liquids have been obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. In the present study, the effects of [γ-Fe₂O₃@SiO₂][BMImCl] and [γ-Fe₂O₃@SiO₂][BMImI] were investigated on the structural properties and function of luciferase using circular dichroism, fluorescence spectroscopy, and bioluminescence assay. Enzyme activity and structural stability increased in the presence of magnetic nanoparticles supported ionic liquids. Furthermore, the effect of ingredients which were used was not considerable on K ₘ value of luciferase for adenosine-5′-triphosphate and also K ₘ value for luciferin.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-014-0730-8</identifier><identifier>PMID: 24492953</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Adenosine Triphosphate - chemistry ; Alternative energy sources ; Animals ; Biochemistry ; Biological and medical sciences ; Bioluminescence ; Biotechnology ; chemical bonding ; Chemistry ; Chemistry and Materials Science ; Enzymatic activity ; enzymatic reactions ; enzyme activity ; Enzyme Stability ; Enzymes ; Enzymes, Immobilized - chemistry ; Ferric Compounds - chemistry ; Fireflies - chemistry ; Fireflies - enzymology ; fluorescence ; Fundamental and applied biological sciences. Psychology ; ingredients ; ionic liquids ; Ionic Liquids - chemistry ; Irradiation ; Kinetics ; luciferase ; Luciferases, Firefly - chemistry ; Luciferin ; Magnetite Nanoparticles - chemistry ; Magnetite Nanoparticles - ultrastructure ; Nanoparticles ; Photinus pyralis ; renewable energy sources ; Silanes - chemistry ; Silica ; Solvents ; spectroscopy</subject><ispartof>Applied biochemistry and biotechnology, 2014-03, Vol.172 (6), p.3116-3127</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-e4da0db36ce3e8eb6e1936d58adff00d83ab22977399d321c57ccd3f2b70bd553</citedby><cites>FETCH-LOGICAL-c496t-e4da0db36ce3e8eb6e1936d58adff00d83ab22977399d321c57ccd3f2b70bd553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-014-0730-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-014-0730-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28577976$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24492953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noori, Ali Reza</creatorcontrib><creatorcontrib>Hosseinkhani, Saman</creatorcontrib><creatorcontrib>Ghiasi, Parisa</creatorcontrib><creatorcontrib>Akbari, Jafar</creatorcontrib><creatorcontrib>Heydari, Akbar</creatorcontrib><title>Magnetic Nanoparticles Supported Ionic Liquids Improve Firefly Luciferase Properties</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Ionic liquids as neoteric solvents, microwave irradiation, and alternative energy source are becoming as a solvent for many enzymatic reactions. We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of luciferase. Magnetic nanoparticles supported ionic liquids have been obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. In the present study, the effects of [γ-Fe₂O₃@SiO₂][BMImCl] and [γ-Fe₂O₃@SiO₂][BMImI] were investigated on the structural properties and function of luciferase using circular dichroism, fluorescence spectroscopy, and bioluminescence assay. Enzyme activity and structural stability increased in the presence of magnetic nanoparticles supported ionic liquids. Furthermore, the effect of ingredients which were used was not considerable on K ₘ value of luciferase for adenosine-5′-triphosphate and also K ₘ value for luciferin.</description><subject>Adenosine Triphosphate - chemistry</subject><subject>Alternative energy sources</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Bioluminescence</subject><subject>Biotechnology</subject><subject>chemical bonding</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymatic activity</subject><subject>enzymatic reactions</subject><subject>enzyme activity</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Ferric Compounds - chemistry</subject><subject>Fireflies - chemistry</subject><subject>Fireflies - enzymology</subject><subject>fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ingredients</subject><subject>ionic liquids</subject><subject>Ionic Liquids - chemistry</subject><subject>Irradiation</subject><subject>Kinetics</subject><subject>luciferase</subject><subject>Luciferases, Firefly - chemistry</subject><subject>Luciferin</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Magnetite Nanoparticles - ultrastructure</subject><subject>Nanoparticles</subject><subject>Photinus pyralis</subject><subject>renewable energy sources</subject><subject>Silanes - chemistry</subject><subject>Silica</subject><subject>Solvents</subject><subject>spectroscopy</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkUFv1DAQhS0EokvhB3CBSAiJS2Bsx3F8RBWFlbYFqe3ZcuzJKlU2Tu0NUv89s8oCVQ-Iky3N92bezGPsNYePHEB_ylwAhxJ4VYKWUDZP2IorZUoQhj9lKxBalkI05oS9yPkWgItG6efsRFSVEUbJFbu-cNsR970vLt0YJ5foO2AuruZpimmPoVjHkaqb_m7uQy7WuynFn1ic9wm74b7YzL7vMLmMxY8UJyQ95pfsWeeGjK-O7ym7Of9yffat3Hz_uj77vCl9Zep9iVVwEFpZe5TYYFsjN7IOqnGh6wBCI10rhNFaGhOk4F5p74PsRKuhDUrJU_Zh6Uue7mbMe7vrs8dhcCPGOVuueK0rpekM_4ECNMYYIPTdI_Q2zmmkRYgCw8lP1RDFF8qnmDMdw06p37l0bznYQzp2ScdSOvaQjj1o3hw7z-0Owx_F7zgIeH8EXPZu6JIbfZ__cpSeNromTixcptK4xfTA4j-mv11EnYvWbRM1vrkiqAIAReON_AUOAK_G</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Noori, Ali Reza</creator><creator>Hosseinkhani, Saman</creator><creator>Ghiasi, Parisa</creator><creator>Akbari, Jafar</creator><creator>Heydari, Akbar</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20140301</creationdate><title>Magnetic Nanoparticles Supported Ionic Liquids Improve Firefly Luciferase Properties</title><author>Noori, Ali Reza ; Hosseinkhani, Saman ; Ghiasi, Parisa ; Akbari, Jafar ; Heydari, Akbar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-e4da0db36ce3e8eb6e1936d58adff00d83ab22977399d321c57ccd3f2b70bd553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenosine Triphosphate - chemistry</topic><topic>Alternative energy sources</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Bioluminescence</topic><topic>Biotechnology</topic><topic>chemical bonding</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymatic activity</topic><topic>enzymatic reactions</topic><topic>enzyme activity</topic><topic>Enzyme Stability</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Ferric Compounds - chemistry</topic><topic>Fireflies - chemistry</topic><topic>Fireflies - enzymology</topic><topic>fluorescence</topic><topic>Fundamental and applied biological sciences. 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We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of luciferase. Magnetic nanoparticles supported ionic liquids have been obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. In the present study, the effects of [γ-Fe₂O₃@SiO₂][BMImCl] and [γ-Fe₂O₃@SiO₂][BMImI] were investigated on the structural properties and function of luciferase using circular dichroism, fluorescence spectroscopy, and bioluminescence assay. Enzyme activity and structural stability increased in the presence of magnetic nanoparticles supported ionic liquids. Furthermore, the effect of ingredients which were used was not considerable on K ₘ value of luciferase for adenosine-5′-triphosphate and also K ₘ value for luciferin.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>24492953</pmid><doi>10.1007/s12010-014-0730-8</doi><tpages>12</tpages></addata></record> |
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| subjects | Adenosine Triphosphate - chemistry Alternative energy sources Animals Biochemistry Biological and medical sciences Bioluminescence Biotechnology chemical bonding Chemistry Chemistry and Materials Science Enzymatic activity enzymatic reactions enzyme activity Enzyme Stability Enzymes Enzymes, Immobilized - chemistry Ferric Compounds - chemistry Fireflies - chemistry Fireflies - enzymology fluorescence Fundamental and applied biological sciences. Psychology ingredients ionic liquids Ionic Liquids - chemistry Irradiation Kinetics luciferase Luciferases, Firefly - chemistry Luciferin Magnetite Nanoparticles - chemistry Magnetite Nanoparticles - ultrastructure Nanoparticles Photinus pyralis renewable energy sources Silanes - chemistry Silica Solvents spectroscopy |
| title | Magnetic Nanoparticles Supported Ionic Liquids Improve Firefly Luciferase Properties |
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