Enzymatic activity of circular sortase A under denaturing conditions: An advanced tool for protein ligation

•Circular S. aureus sortase A has been expressed and purified.•Enzymatic activity of the circular enzyme is not affected by the modification.•Circular enzyme is stable and more stable than the linear one in the presence of urea.•Circular enzyme exhibits enhanced activity in the denaturing conditions...

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Veröffentlicht in:	Biochemical engineering journal 2014-01, Vol.82, p.200-209
Hauptverfasser:	Zhulenkovs, D., Jaudzems, K., Zajakina, A., Leonchiks, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Denaturing conditions Enzyme stability Escherichia coli Fundamental and applied biological sciences. Psychology Intein-mediated cyclization Protein ligation Sortase A Staphylococcus aureus Synechocystis
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