Flow cytometry as a method for assaying the biological activity of phytotoxins
A flow cytometric method has been developed for the qualitative and quantitative evaluation of the biological activities of phytotoxins from plant pathogenic fungi. The method utilized fresh wheat ( Triticum aestivum L.) leaf protoplast preparations treated with purified phytotoxins, triticone A-B a...
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| Veröffentlicht in: | Plant science (Limerick) 1988, Vol.56 (2), p.183-188 |
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| container_title | Plant science (Limerick) |
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| creator | Berglund, Deborah L. Strobel, Scott Sugawara, Fumio Strobel, Gary A. |
| description | A flow cytometric method has been developed for the qualitative and quantitative evaluation of the biological activities of phytotoxins from plant pathogenic fungi. The method utilized fresh wheat (
Triticum aestivum L.) leaf protoplast preparations treated with purified phytotoxins, triticone A-B and triticone D. Subsequently, protoplasts were exposed to fluorescein diacetate, and analyzed by flow cytometry. Information acquired included fluorescence owing to esterase activity on fluorescein diacetate, and chlorophyll autofluorescence. Results indicate that triticone A-B has a rapid dose-dependent toxic effect on wheat protoplasts but triticone D has no toxic effect. This method can also yield information on the mechanism of action of phytotoxins that are relatively unstable or available only in small quantities. |
| doi_str_mv | 10.1016/0168-9452(88)90033-7 |
| format | Article |
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Triticum aestivum L.) leaf protoplast preparations treated with purified phytotoxins, triticone A-B and triticone D. Subsequently, protoplasts were exposed to fluorescein diacetate, and analyzed by flow cytometry. Information acquired included fluorescence owing to esterase activity on fluorescein diacetate, and chlorophyll autofluorescence. Results indicate that triticone A-B has a rapid dose-dependent toxic effect on wheat protoplasts but triticone D has no toxic effect. This method can also yield information on the mechanism of action of phytotoxins that are relatively unstable or available only in small quantities.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/0168-9452(88)90033-7</identifier><language>eng</language><publisher>Elsevier Ireland Ltd</publisher><subject>flow cytometry ; fluorescein diacetate ; phytotoxins ; protoplasts ; Triticum aestivum</subject><ispartof>Plant science (Limerick), 1988, Vol.56 (2), p.183-188</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-a8b4d568434445511a74815e2369283e7cee154b66bea64e35da8f3e9327faac3</citedby><cites>FETCH-LOGICAL-c335t-a8b4d568434445511a74815e2369283e7cee154b66bea64e35da8f3e9327faac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-9452(88)90033-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27910,27911,27912,45982</link.rule.ids></links><search><creatorcontrib>Berglund, Deborah L.</creatorcontrib><creatorcontrib>Strobel, Scott</creatorcontrib><creatorcontrib>Sugawara, Fumio</creatorcontrib><creatorcontrib>Strobel, Gary A.</creatorcontrib><title>Flow cytometry as a method for assaying the biological activity of phytotoxins</title><title>Plant science (Limerick)</title><description>A flow cytometric method has been developed for the qualitative and quantitative evaluation of the biological activities of phytotoxins from plant pathogenic fungi. The method utilized fresh wheat (
Triticum aestivum L.) leaf protoplast preparations treated with purified phytotoxins, triticone A-B and triticone D. Subsequently, protoplasts were exposed to fluorescein diacetate, and analyzed by flow cytometry. Information acquired included fluorescence owing to esterase activity on fluorescein diacetate, and chlorophyll autofluorescence. Results indicate that triticone A-B has a rapid dose-dependent toxic effect on wheat protoplasts but triticone D has no toxic effect. This method can also yield information on the mechanism of action of phytotoxins that are relatively unstable or available only in small quantities.</description><subject>flow cytometry</subject><subject>fluorescein diacetate</subject><subject>phytotoxins</subject><subject>protoplasts</subject><subject>Triticum aestivum</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LAzEQxYMoWKvfwENOoofV_N3NXgQRq0LRi55DNjvbRrZNTdLqfnuzVDx6GN4MvPdgfgidU3JNCS1v8qiiFpJdKnVVE8J5UR2gCVUVLxiT9SGa_FmO0UmMH4QQJmU1QS-z3n9hOyS_ghQGbCI2OK9L3-LOh3xHM7j1Aqcl4Mb53i-cNT02NrmdSwP2Hd4sczz5b7eOp-ioM32Es1-dovfZw9v9UzF_fXy-v5sXlnOZCqMa0cpSCS6EkJJSUwlFJTBe1kxxqCwAlaIpywZMKYDL1qiOQ81Z1Rlj-RRd7Hs3wX9uISa9ctFC35s1-G3UVNKSMCayUeyNNvgYA3R6E9zKhEFTokd4eiSjRzJaZR3h6SrHbvcxyE_sHAQdrYO1hdYFsEm33v1f8AMXMXZr</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Berglund, Deborah L.</creator><creator>Strobel, Scott</creator><creator>Sugawara, Fumio</creator><creator>Strobel, Gary A.</creator><general>Elsevier Ireland Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>1988</creationdate><title>Flow cytometry as a method for assaying the biological activity of phytotoxins</title><author>Berglund, Deborah L. ; Strobel, Scott ; Sugawara, Fumio ; Strobel, Gary A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-a8b4d568434445511a74815e2369283e7cee154b66bea64e35da8f3e9327faac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>flow cytometry</topic><topic>fluorescein diacetate</topic><topic>phytotoxins</topic><topic>protoplasts</topic><topic>Triticum aestivum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berglund, Deborah L.</creatorcontrib><creatorcontrib>Strobel, Scott</creatorcontrib><creatorcontrib>Sugawara, Fumio</creatorcontrib><creatorcontrib>Strobel, Gary A.</creatorcontrib><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Berglund, Deborah L.</au><au>Strobel, Scott</au><au>Sugawara, Fumio</au><au>Strobel, Gary A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow cytometry as a method for assaying the biological activity of phytotoxins</atitle><jtitle>Plant science (Limerick)</jtitle><date>1988</date><risdate>1988</risdate><volume>56</volume><issue>2</issue><spage>183</spage><epage>188</epage><pages>183-188</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><abstract>A flow cytometric method has been developed for the qualitative and quantitative evaluation of the biological activities of phytotoxins from plant pathogenic fungi. The method utilized fresh wheat (
Triticum aestivum L.) leaf protoplast preparations treated with purified phytotoxins, triticone A-B and triticone D. Subsequently, protoplasts were exposed to fluorescein diacetate, and analyzed by flow cytometry. Information acquired included fluorescence owing to esterase activity on fluorescein diacetate, and chlorophyll autofluorescence. Results indicate that triticone A-B has a rapid dose-dependent toxic effect on wheat protoplasts but triticone D has no toxic effect. This method can also yield information on the mechanism of action of phytotoxins that are relatively unstable or available only in small quantities.</abstract><pub>Elsevier Ireland Ltd</pub><doi>10.1016/0168-9452(88)90033-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-9452 |
| ispartof | Plant science (Limerick), 1988, Vol.56 (2), p.183-188 |
| issn | 0168-9452 1873-2259 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15160224 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | flow cytometry fluorescein diacetate phytotoxins protoplasts Triticum aestivum |
| title | Flow cytometry as a method for assaying the biological activity of phytotoxins |
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