Purification, primary structure characterization, and cellular distribution of two forms of cellular retinol-binding protein, type II from adult small intestine

Purification, primary structure characterization, and cellular distribution of two forms of cellular retinol-binding protein, type II from adult small intestine

Cellular retinol-binding protein type II (CRBP(II)) is a major protein in the small intestine, accounting for more than 1% of the soluble protein recovered from rat jujunal mucosa. Two forms of the protein, called CRBP(II)A and CRBP(II)B, were purified from rat small intestine using a three-column p...

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Veröffentlicht in:The Journal of biological chemistry 1989-01, Vol.264 (7), p.4212-4221
Hauptverfasser: Schaefer, W H, Kakkad, B, Crow, JA, Blair, IA, Ong, DE
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small intestine
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container_end_page 4221
container_issue 7
container_start_page 4212
container_title The Journal of biological chemistry
container_volume 264
creator Schaefer, W H
Kakkad, B
Crow, JA
Blair, IA
Ong, DE
description Cellular retinol-binding protein type II (CRBP(II)) is a major protein in the small intestine, accounting for more than 1% of the soluble protein recovered from rat jujunal mucosa. Two forms of the protein, called CRBP(II)A and CRBP(II)B, were purified from rat small intestine using a three-column procedure. The two forms were present in equal abundance. The primary structures of CRBP(II)A and CRBP(II)B were determined using a combination of techniques including amino acid composition and sequence analyses, and fast atom bombardment and gas chromatography-electron impact mass spectrometry. The results provide a basis for investigating the possibility of different roles of CRBP(II)A and CRBP(II)B within cells, as well as the importance of acetylation of the amino terminus for these biological functions.
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects small intestine
title Purification, primary structure characterization, and cellular distribution of two forms of cellular retinol-binding protein, type II from adult small intestine
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