Site-directed mutagenesis with Escherichia coli DNA polymerase III holoenzyme

Escherichia coli DNA polymerase III holoenzyme was used to synthesize double-stranded DNA from M 13 single-stranded DNA hybridized to a phosphorylated synthetic oligodeoxynucleotide containing a nucleotide substitution. The resulting DNA was transfected into E. coli JM101 without further treatment....

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Veröffentlicht in:	Gene 1988-01, Vol.62 (1), p.135-139
Hauptverfasser:	Tsurusluta, Naoya, Maki, Hisaji, Korn, Laurence Jay
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - metabolism Base Sequence Biological and medical sciences Biotechnology Coliphages - genetics DNA Polymerase III - metabolism DNA, Recombinant DNA, Viral - biosynthesis DNA, Viral - genetics DNA-Directed DNA Polymerase - metabolism Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic technics Genetic Techniques in vitro DNA synthesis Methods. Procedures. Technologies Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis. Repair Mutant screening Mutation phage M 13 phage M13 Prokaryotes Recombinant DNA S SB protein single-stranded DNA synthetic oligodeoxy- nucleotide
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