Plasmodium falciparum Gene Encoding a Protein Similar to the 78-kDa Rat Glucose-Regulated Stress Protein
Genes homologous to heat shock protein 70 have been described in parasitic protozoa. It has been proposed that they may be important to the parasite as it moves from the vertebrate host at 37 degrees C to the insect. We now describe a genomic DNA clone isolated from Plasmodium falciparum that encode...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-09, Vol.85 (17), p.6277-6281 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Kumar, Nirbhay Syin, Chiang Carter, Richard Quakyi, Isabella Miller, Louis H. |
| description | Genes homologous to heat shock protein 70 have been described in parasitic protozoa. It has been proposed that they may be important to the parasite as it moves from the vertebrate host at 37 degrees C to the insect. We now describe a genomic DNA clone isolated from Plasmodium falciparum that encodes a protein similar in sequence to a mammalian heat shock-related protein, the 78-kDa glucose-regulated protein of rat and hamster. The gene is expressed during the erythrocytic stage in both asexual and sexual parasites (RNA blot analysis) and a 72-kDa protein is immunoprecipitated from erythrocytic stage parasites. Importantly, the sequence of the clone is similar to the canonical sequence at the carboxyl termini of glucose-regulated proteins of mammals that determines their localization within endoplasmic reticulum. Since the parasite sequence has only three (Asp-Glu-Leu) of the four carboxylterminal amino acids, its location and its function within the parasite remain to be determined. |
| doi_str_mv | 10.1073/pnas.85.17.6277 |
| format | Article |
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Genome ; Genomics ; Heat shock proteins ; Heat-Shock Proteins - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molecular Weight ; Parasites ; Plasmodium falciparum - genetics ; Proteins ; Proteins - genetics ; Proteins - isolation & purification ; Rats ; RNA ; Sequence Homology, Nucleic Acid</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-09, Vol.85 (17), p.6277-6281</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-6d69559870cd51f1768df3e7c9419d57c37a0eb91d4c587db1e69a76024f310c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/17.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/31999$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/31999$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7184172$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3045815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Nirbhay</creatorcontrib><creatorcontrib>Syin, Chiang</creatorcontrib><creatorcontrib>Carter, Richard</creatorcontrib><creatorcontrib>Quakyi, Isabella</creatorcontrib><creatorcontrib>Miller, Louis H.</creatorcontrib><title>Plasmodium falciparum Gene Encoding a Protein Similar to the 78-kDa Rat Glucose-Regulated Stress Protein</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Genes homologous to heat shock protein 70 have been described in parasitic protozoa. It has been proposed that they may be important to the parasite as it moves from the vertebrate host at 37 degrees C to the insect. We now describe a genomic DNA clone isolated from Plasmodium falciparum that encodes a protein similar in sequence to a mammalian heat shock-related protein, the 78-kDa glucose-regulated protein of rat and hamster. The gene is expressed during the erythrocytic stage in both asexual and sexual parasites (RNA blot analysis) and a 72-kDa protein is immunoprecipitated from erythrocytic stage parasites. Importantly, the sequence of the clone is similar to the canonical sequence at the carboxyl termini of glucose-regulated proteins of mammals that determines their localization within endoplasmic reticulum. Since the parasite sequence has only three (Asp-Glu-Leu) of the four carboxylterminal amino acids, its location and its function within the parasite remain to be determined.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antiserum</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>Endoplasmic reticulum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gametes</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>Genomics</subject><subject>Heat shock proteins</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Parasites</subject><subject>Plasmodium falciparum - genetics</subject><subject>Proteins</subject><subject>Proteins - genetics</subject><subject>Proteins - isolation & purification</subject><subject>Rats</subject><subject>RNA</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhjIQE8gHBKVtP4s8jKmVBqkTVwtny2pM2xUm2tiPBf09WmxZxgdOM9H7zZjSPkJfA1sBUc7IbXF5rsQa1lrVSj8gKmIFKcsMekxVjtao0r_lT8iznW8aYEZodkaOGcaFBrMjNRXS5H0M39bR10Xc7l-Z2gwPSs8HPwnBNHb1IY8FuoFdd30WXaBlpuUGqdPXjo6OXrtBNnPyYsbrE6ym6goFelYQ5348-J09m_4wvlnpMvn86-3b6uTr_uvly-uG88lyqUskgjRBGK-aDgBaU1KFtUHnDwQShfKMcw62BwL3QKmwBpXFKspq3DTDfHJN3B99dGu8mzMX2XfYYoxtwnLJVujFaavgvCAJ4LTWbwZMD6NOYc8LW7lLXu_TLArP7EOw-BKuFBWX3IcwTrxfradtjeOCXr8_620V32bvYJjf4Lj9gCjQHVc_YmwXb-9-rf-15_0_AtlOMBX-WmXx1IG9zGdOfg8AY0_wGz-uvgw</recordid><startdate>19880901</startdate><enddate>19880901</enddate><creator>Kumar, Nirbhay</creator><creator>Syin, Chiang</creator><creator>Carter, Richard</creator><creator>Quakyi, Isabella</creator><creator>Miller, Louis H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19880901</creationdate><title>Plasmodium falciparum Gene Encoding a Protein Similar to the 78-kDa Rat Glucose-Regulated Stress Protein</title><author>Kumar, Nirbhay ; Syin, Chiang ; Carter, Richard ; Quakyi, Isabella ; Miller, Louis H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-6d69559870cd51f1768df3e7c9419d57c37a0eb91d4c587db1e69a76024f310c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antiserum</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>Endoplasmic reticulum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gametes</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>Genomics</topic><topic>Heat shock proteins</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Parasites</topic><topic>Plasmodium falciparum - genetics</topic><topic>Proteins</topic><topic>Proteins - genetics</topic><topic>Proteins - isolation & purification</topic><topic>Rats</topic><topic>RNA</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Nirbhay</creatorcontrib><creatorcontrib>Syin, Chiang</creatorcontrib><creatorcontrib>Carter, Richard</creatorcontrib><creatorcontrib>Quakyi, Isabella</creatorcontrib><creatorcontrib>Miller, Louis H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Nirbhay</au><au>Syin, Chiang</au><au>Carter, Richard</au><au>Quakyi, Isabella</au><au>Miller, Louis H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmodium falciparum Gene Encoding a Protein Similar to the 78-kDa Rat Glucose-Regulated Stress Protein</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-09-01</date><risdate>1988</risdate><volume>85</volume><issue>17</issue><spage>6277</spage><epage>6281</epage><pages>6277-6281</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Genes homologous to heat shock protein 70 have been described in parasitic protozoa. It has been proposed that they may be important to the parasite as it moves from the vertebrate host at 37 degrees C to the insect. We now describe a genomic DNA clone isolated from Plasmodium falciparum that encodes a protein similar in sequence to a mammalian heat shock-related protein, the 78-kDa glucose-regulated protein of rat and hamster. The gene is expressed during the erythrocytic stage in both asexual and sexual parasites (RNA blot analysis) and a 72-kDa protein is immunoprecipitated from erythrocytic stage parasites. Importantly, the sequence of the clone is similar to the canonical sequence at the carboxyl termini of glucose-regulated proteins of mammals that determines their localization within endoplasmic reticulum. Since the parasite sequence has only three (Asp-Glu-Leu) of the four carboxylterminal amino acids, its location and its function within the parasite remain to be determined.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3045815</pmid><doi>10.1073/pnas.85.17.6277</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | JSTOR Archive Collection A-Z Listing; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Animals Antiserum Base Sequence Biological and medical sciences Cloning, Molecular DNA Endoplasmic reticulum Fundamental and applied biological sciences. Psychology Gametes Genes Genes. Genome Genomics Heat shock proteins Heat-Shock Proteins - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecular Weight Parasites Plasmodium falciparum - genetics Proteins Proteins - genetics Proteins - isolation & purification Rats RNA Sequence Homology, Nucleic Acid |
| title | Plasmodium falciparum Gene Encoding a Protein Similar to the 78-kDa Rat Glucose-Regulated Stress Protein |
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