Mechanical properties of cancer cytoskeleton depend on actin filaments to microtubules content: Investigating different grades of colon cancer cell lines
Abstract Biomechanical properties of cancer cells have been proposed as promising biomarkers to investigate cancer progression. Cytoskeletal reorganization alters these characteristics in different grades of cancer cells. In the present study based on the micropipette aspiration method, whole body e...
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| Veröffentlicht in: | Journal of biomechanics 2014-01, Vol.47 (2), p.373-379 |
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| container_title | Journal of biomechanics |
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| creator | Pachenari, M Seyedpour, S.M Janmaleki, M Shayan, S. Babazadeh Taranejoo, S Hosseinkhani, H |
| description | Abstract Biomechanical properties of cancer cells have been proposed as promising biomarkers to investigate cancer progression. Cytoskeletal reorganization alters these characteristics in different grades of cancer cells. In the present study based on the micropipette aspiration method, whole body evaluation for two different colon cancer cells was performed to determine viscoelastic parameters of the cells. A finite element model was developed for verification of experiments and predicting some behaviors of cells. Western blot analysis and fluorescence intensity for actin microfilaments and microtubules were performed to measure cell content of the proteins. It was illustrated that the proportion of microtubules and actin microfilaments is different in grade I and grade IV colon cancer cells in a manner that microtubules attain an effectual role in progressive reorganization of cytoskeleton in transition from nonaggressive to malignant phenotypes in cancer cells. Furthermore, it was concluded that larger instantaneous Young's modulus value for high grade cells is related to the existence of extensively build-up actin networks at the cell cortex. Based on the cell mechanics results, a simple parameter is suggested for sorting different grades of colon cancer cells. |
| doi_str_mv | 10.1016/j.jbiomech.2013.11.020 |
| format | Article |
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Babazadeh ; Taranejoo, S ; Hosseinkhani, H</creator><creatorcontrib>Pachenari, M ; Seyedpour, S.M ; Janmaleki, M ; Shayan, S. Babazadeh ; Taranejoo, S ; Hosseinkhani, H</creatorcontrib><description>Abstract Biomechanical properties of cancer cells have been proposed as promising biomarkers to investigate cancer progression. Cytoskeletal reorganization alters these characteristics in different grades of cancer cells. In the present study based on the micropipette aspiration method, whole body evaluation for two different colon cancer cells was performed to determine viscoelastic parameters of the cells. A finite element model was developed for verification of experiments and predicting some behaviors of cells. Western blot analysis and fluorescence intensity for actin microfilaments and microtubules were performed to measure cell content of the proteins. It was illustrated that the proportion of microtubules and actin microfilaments is different in grade I and grade IV colon cancer cells in a manner that microtubules attain an effectual role in progressive reorganization of cytoskeleton in transition from nonaggressive to malignant phenotypes in cancer cells. Furthermore, it was concluded that larger instantaneous Young's modulus value for high grade cells is related to the existence of extensively build-up actin networks at the cell cortex. Based on the cell mechanics results, a simple parameter is suggested for sorting different grades of colon cancer cells.</description><identifier>ISSN: 0021-9290</identifier><identifier>EISSN: 1873-2380</identifier><identifier>DOI: 10.1016/j.jbiomech.2013.11.020</identifier><identifier>PMID: 24315289</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Accumulation ; Actin Cytoskeleton - metabolism ; Actins - metabolism ; Albendazole - pharmacology ; Biomechanics ; Blotting, Western ; Cancer ; Cancer cell mechanics ; Cell Movement - drug effects ; Colon ; Colonic Neoplasms - pathology ; Colorectal cancer ; Cytoskeleton ; Cytoskeleton - pathology ; Elastic Modulus - physiology ; Elasticity ; Filaments ; Finite Element Analysis ; HT29 Cells ; Humans ; Mathematical models ; Medical research ; Micropipette aspiration ; Microtubules - metabolism ; Microtubules - pathology ; Networks ; Physical Medicine and Rehabilitation ; Rate of deformability ; Sorting ; Viscosity</subject><ispartof>Journal of biomechanics, 2014-01, Vol.47 (2), p.373-379</ispartof><rights>Elsevier Ltd</rights><rights>2013 Elsevier Ltd</rights><rights>2013 Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-edf0edf958fcdad84354d11c1beebfb96ee6561e0500116440c759e39ec1753c3</citedby><cites>FETCH-LOGICAL-c484t-edf0edf958fcdad84354d11c1beebfb96ee6561e0500116440c759e39ec1753c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1475171219?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24315289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pachenari, M</creatorcontrib><creatorcontrib>Seyedpour, S.M</creatorcontrib><creatorcontrib>Janmaleki, M</creatorcontrib><creatorcontrib>Shayan, S. Babazadeh</creatorcontrib><creatorcontrib>Taranejoo, S</creatorcontrib><creatorcontrib>Hosseinkhani, H</creatorcontrib><title>Mechanical properties of cancer cytoskeleton depend on actin filaments to microtubules content: Investigating different grades of colon cancer cell lines</title><title>Journal of biomechanics</title><addtitle>J Biomech</addtitle><description>Abstract Biomechanical properties of cancer cells have been proposed as promising biomarkers to investigate cancer progression. Cytoskeletal reorganization alters these characteristics in different grades of cancer cells. In the present study based on the micropipette aspiration method, whole body evaluation for two different colon cancer cells was performed to determine viscoelastic parameters of the cells. A finite element model was developed for verification of experiments and predicting some behaviors of cells. Western blot analysis and fluorescence intensity for actin microfilaments and microtubules were performed to measure cell content of the proteins. It was illustrated that the proportion of microtubules and actin microfilaments is different in grade I and grade IV colon cancer cells in a manner that microtubules attain an effectual role in progressive reorganization of cytoskeleton in transition from nonaggressive to malignant phenotypes in cancer cells. Furthermore, it was concluded that larger instantaneous Young's modulus value for high grade cells is related to the existence of extensively build-up actin networks at the cell cortex. Based on the cell mechanics results, a simple parameter is suggested for sorting different grades of colon cancer cells.</description><subject>Accumulation</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Actins - metabolism</subject><subject>Albendazole - pharmacology</subject><subject>Biomechanics</subject><subject>Blotting, Western</subject><subject>Cancer</subject><subject>Cancer cell mechanics</subject><subject>Cell Movement - drug effects</subject><subject>Colon</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal cancer</subject><subject>Cytoskeleton</subject><subject>Cytoskeleton - pathology</subject><subject>Elastic Modulus - physiology</subject><subject>Elasticity</subject><subject>Filaments</subject><subject>Finite Element Analysis</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Mathematical models</subject><subject>Medical research</subject><subject>Micropipette aspiration</subject><subject>Microtubules - metabolism</subject><subject>Microtubules - pathology</subject><subject>Networks</subject><subject>Physical Medicine and Rehabilitation</subject><subject>Rate of deformability</subject><subject>Sorting</subject><subject>Viscosity</subject><issn>0021-9290</issn><issn>1873-2380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkstu1DAUhiMEosPAK1SW2LDJcI4T58ICUVVcKhWxANaWY58MnibxYDuV5lF4WxzNDEjddGHZ0vn8n8t_suwSYYOA1dvdZtdZN5L-teGAxQZxAxyeZCts6iLnRQNPsxUAx7zlLVxkL0LYAUBd1u3z7IKXBQretKvsz9ckoSar1cD23u3JR0uBuZ5pNWnyTB-iC3c0UHQTM7SnybD0UjraifV2UCNNMbDo2Gi1d3Hu5iEJaDfFFHjHbqZ7CtFuVeK3zNi-J58CbOuVOSVyQxI8p6NhYIOdKLzMnvVqCPTqdK-zn58-_rj-kt9--3xzfXWb67IpY06mh3Ra0fTaKNOUhSgNosaOqOu7tiKqRIUEAgCxKkvQtWipaEljLQpdrLM3R93U_u851SpHG5Yy1ERuDhIFFmVbCuCPo2ULNdY86a6z1w_QnZv9lBpJVC2wRo5toqojlSYXgqde7r0dlT9IBLn4LHfy7LNcfJaIMvmcPl6e5OduJPPv29nYBHw4ApRGd2_Jy6AtpREb60lHaZx9PMf7BxI6GbNsyh0dKPzvRwYuQX5ftm1ZNiwARM2x-AuOqtQz</recordid><startdate>20140122</startdate><enddate>20140122</enddate><creator>Pachenari, M</creator><creator>Seyedpour, S.M</creator><creator>Janmaleki, M</creator><creator>Shayan, S. 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A finite element model was developed for verification of experiments and predicting some behaviors of cells. Western blot analysis and fluorescence intensity for actin microfilaments and microtubules were performed to measure cell content of the proteins. It was illustrated that the proportion of microtubules and actin microfilaments is different in grade I and grade IV colon cancer cells in a manner that microtubules attain an effectual role in progressive reorganization of cytoskeleton in transition from nonaggressive to malignant phenotypes in cancer cells. Furthermore, it was concluded that larger instantaneous Young's modulus value for high grade cells is related to the existence of extensively build-up actin networks at the cell cortex. Based on the cell mechanics results, a simple parameter is suggested for sorting different grades of colon cancer cells.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>24315289</pmid><doi>10.1016/j.jbiomech.2013.11.020</doi><tpages>7</tpages></addata></record> |
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| subjects | Accumulation Actin Cytoskeleton - metabolism Actins - metabolism Albendazole - pharmacology Biomechanics Blotting, Western Cancer Cancer cell mechanics Cell Movement - drug effects Colon Colonic Neoplasms - pathology Colorectal cancer Cytoskeleton Cytoskeleton - pathology Elastic Modulus - physiology Elasticity Filaments Finite Element Analysis HT29 Cells Humans Mathematical models Medical research Micropipette aspiration Microtubules - metabolism Microtubules - pathology Networks Physical Medicine and Rehabilitation Rate of deformability Sorting Viscosity |
| title | Mechanical properties of cancer cytoskeleton depend on actin filaments to microtubules content: Investigating different grades of colon cancer cell lines |
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