Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: A potential low cost source of the enzyme
•Pectinase has many important applications in various industrial processes.•Alcohol/salt based ATPS was used to purify pectinase from mango peel.•Purification factor, yield, partition coefficient and selectivity were optimised.•Pectinase from mango peel was recovered with a high purification factor...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2013-07, Vol.931, p.17-22 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Amid, Mehrnoush Abdul Manap, Mohd Yazid Mustafa, Shuhaimi |
| description | •Pectinase has many important applications in various industrial processes.•Alcohol/salt based ATPS was used to purify pectinase from mango peel.•Purification factor, yield, partition coefficient and selectivity were optimised.•Pectinase from mango peel was recovered with a high purification factor (11.7) and a high yield (97.1%).
As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%. |
| doi_str_mv | 10.1016/j.jchromb.2013.05.009 |
| format | Article |
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As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2013.05.009</identifier><identifier>PMID: 23770734</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>1-propanol ; ammonium sulfate ; Analysis of Variance ; Aqueous organic phase system ; Biotechnology ; chromatography ; Electrophoresis, Polyacrylamide Gel ; Enzymes ; Ethanol ; Ethyl alcohol ; feedstocks ; Fruit - chemistry ; Fruit - enzymology ; Hydrogen-Ion Concentration ; isopropyl alcohol ; Mangifera - chemistry ; Mangifera - enzymology ; Mangifera indica ; Mango peel ; mangoes ; Organic solvent ; Partitions ; Pectinase ; Phosphates ; polygalacturonase ; Polygalacturonase - analysis ; Polygalacturonase - chemistry ; Polygalacturonase - isolation & purification ; Polygalacturonase - metabolism ; Potassium Compounds ; potassium phosphates ; Purification ; purification methods ; Sodium Chloride ; sodium citrate ; sodium phosphate ; Solid Waste ; Wastes</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2013-07, Vol.931, p.17-22</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-e5e04d7ef2b486eb1dba3736e5146338b74f114c2e749b06a9cf73f2520825f73</citedby><cites>FETCH-LOGICAL-c469t-e5e04d7ef2b486eb1dba3736e5146338b74f114c2e749b06a9cf73f2520825f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023213002845$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23770734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amid, Mehrnoush</creatorcontrib><creatorcontrib>Abdul Manap, Mohd Yazid</creatorcontrib><creatorcontrib>Mustafa, Shuhaimi</creatorcontrib><title>Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: A potential low cost source of the enzyme</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>•Pectinase has many important applications in various industrial processes.•Alcohol/salt based ATPS was used to purify pectinase from mango peel.•Purification factor, yield, partition coefficient and selectivity were optimised.•Pectinase from mango peel was recovered with a high purification factor (11.7) and a high yield (97.1%).
As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%.</description><subject>1-propanol</subject><subject>ammonium sulfate</subject><subject>Analysis of Variance</subject><subject>Aqueous organic phase system</subject><subject>Biotechnology</subject><subject>chromatography</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Ethyl alcohol</subject><subject>feedstocks</subject><subject>Fruit - chemistry</subject><subject>Fruit - enzymology</subject><subject>Hydrogen-Ion Concentration</subject><subject>isopropyl alcohol</subject><subject>Mangifera - chemistry</subject><subject>Mangifera - enzymology</subject><subject>Mangifera indica</subject><subject>Mango peel</subject><subject>mangoes</subject><subject>Organic solvent</subject><subject>Partitions</subject><subject>Pectinase</subject><subject>Phosphates</subject><subject>polygalacturonase</subject><subject>Polygalacturonase - analysis</subject><subject>Polygalacturonase - chemistry</subject><subject>Polygalacturonase - isolation & purification</subject><subject>Polygalacturonase - metabolism</subject><subject>Potassium Compounds</subject><subject>potassium phosphates</subject><subject>Purification</subject><subject>purification methods</subject><subject>Sodium Chloride</subject><subject>sodium citrate</subject><subject>sodium phosphate</subject><subject>Solid Waste</subject><subject>Wastes</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhCMEYn_gEYA-LocE_yRxwgWtRiwgDQIJVuJmOU57xsPEDnayq-FheFYczcB1T12Hr8vVrix7QUlBCa3f7Iqd3gY_dAUjlBekKghpH2XntBE856L-8TjpSpCcMM7OsosYd4RQQQR_mp0xLhZVnmd_vs7BGqvVZL0Db2BEPVmnIoJJ7jAot_Fw9TkNazAosK5PNKwL0HcFrLb-p3LKvYZ7FSeEOVq3AeVA_ZrRzxF82ChnNYzbxTIeEjS8hWsY_YRusmoPe38P2scJop-DxiXDtEVA9_sw4LPsiVH7iM9P8zK7vXn_ffUxX3_58Gl1vc51WbdTjhWSshdoWFc2NXa07xQXvMaKljXnTSdKQ2mpGYqy7UitWm0EN6xipGFVkpfZ1dF3DD4lj5McbNS43yu3nCFpRXkpRFnTh1He8oZy1i5odUR18DEGNHIMdlDhICmRS4tyJ08tyqVFSSqZWkx7L09PzN2A_f-tf7Ul4NURMMpLtQk2yttvyaEmhNSiZU0i3h0JTL92ZzHIqC06jb0NqWHZe_tAiL93UrrC</recordid><startdate>20130715</startdate><enddate>20130715</enddate><creator>Amid, Mehrnoush</creator><creator>Abdul Manap, Mohd Yazid</creator><creator>Mustafa, Shuhaimi</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20130715</creationdate><title>Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: A potential low cost source of the enzyme</title><author>Amid, Mehrnoush ; Abdul Manap, Mohd Yazid ; Mustafa, Shuhaimi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-e5e04d7ef2b486eb1dba3736e5146338b74f114c2e749b06a9cf73f2520825f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>1-propanol</topic><topic>ammonium sulfate</topic><topic>Analysis of Variance</topic><topic>Aqueous organic phase system</topic><topic>Biotechnology</topic><topic>chromatography</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>Ethyl alcohol</topic><topic>feedstocks</topic><topic>Fruit - chemistry</topic><topic>Fruit - enzymology</topic><topic>Hydrogen-Ion Concentration</topic><topic>isopropyl alcohol</topic><topic>Mangifera - chemistry</topic><topic>Mangifera - enzymology</topic><topic>Mangifera indica</topic><topic>Mango peel</topic><topic>mangoes</topic><topic>Organic solvent</topic><topic>Partitions</topic><topic>Pectinase</topic><topic>Phosphates</topic><topic>polygalacturonase</topic><topic>Polygalacturonase - analysis</topic><topic>Polygalacturonase - chemistry</topic><topic>Polygalacturonase - isolation & purification</topic><topic>Polygalacturonase - metabolism</topic><topic>Potassium Compounds</topic><topic>potassium phosphates</topic><topic>Purification</topic><topic>purification methods</topic><topic>Sodium Chloride</topic><topic>sodium citrate</topic><topic>sodium phosphate</topic><topic>Solid Waste</topic><topic>Wastes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amid, Mehrnoush</creatorcontrib><creatorcontrib>Abdul Manap, Mohd Yazid</creatorcontrib><creatorcontrib>Mustafa, Shuhaimi</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amid, Mehrnoush</au><au>Abdul Manap, Mohd Yazid</au><au>Mustafa, Shuhaimi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: A potential low cost source of the enzyme</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2013-07-15</date><risdate>2013</risdate><volume>931</volume><spage>17</spage><epage>22</epage><pages>17-22</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>•Pectinase has many important applications in various industrial processes.•Alcohol/salt based ATPS was used to purify pectinase from mango peel.•Purification factor, yield, partition coefficient and selectivity were optimised.•Pectinase from mango peel was recovered with a high purification factor (11.7) and a high yield (97.1%).
As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23770734</pmid><doi>10.1016/j.jchromb.2013.05.009</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1570-0232 1873-376X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 1-propanol ammonium sulfate Analysis of Variance Aqueous organic phase system Biotechnology chromatography Electrophoresis, Polyacrylamide Gel Enzymes Ethanol Ethyl alcohol feedstocks Fruit - chemistry Fruit - enzymology Hydrogen-Ion Concentration isopropyl alcohol Mangifera - chemistry Mangifera - enzymology Mangifera indica Mango peel mangoes Organic solvent Partitions Pectinase Phosphates polygalacturonase Polygalacturonase - analysis Polygalacturonase - chemistry Polygalacturonase - isolation & purification Polygalacturonase - metabolism Potassium Compounds potassium phosphates Purification purification methods Sodium Chloride sodium citrate sodium phosphate Solid Waste Wastes |
| title | Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: A potential low cost source of the enzyme |
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