Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding
Abstract The effects of chronic ethanol administration on the hepatic surface membrane were examined. The binding of the lectin, concanavalin A (Con A), to isolated hepatocytes was used to ascertain changes in the hepatic plasma membrane, especially in regard to glycoprotein composition, due to chro...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1987-05, Vol.185 (1), p.1-5 |
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| creator | Metcalf, Jordan P. Casey, Carol A. Sorrell, Michael F. Tuma, Dean J. |
| description | Abstract
The effects of chronic ethanol administration on the hepatic surface membrane were examined. The binding of the lectin, concanavalin A (Con A), to isolated hepatocytes was used to ascertain changes in the hepatic plasma membrane, especially in regard to glycoprotein composition, due to chronic ethanol feeding. Hepatocytes, isolated from rats fed ethanol for 5 to 7 weeks, had a decreased ability to bind Con A when compared to hepatocytes from either the pair-fed controls or ad libitum chow-fed rats. Since decreased Con A binding was more apparent at high Con A concentrations, reduced lectin binding likely reflected changes in the composition of surface membrane glycoproteins in the livers of the ethanol-fed rats. When ethanol (50 mM) was added to the incubation medium containing hepatocytes from ethanol-fed rats, pair-fed controls, or chow-fed rats, no effects on Con A binding were observed. These results indicate that chronic ethanol administration induces changes in the oligosaccharide chains of plasma membrane glycoproteins in the liver. Such alterations may play a role in the pathogenesis of alcoholic liver disease. |
| doi_str_mv | 10.3181/00379727-185-42508 |
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The effects of chronic ethanol administration on the hepatic surface membrane were examined. The binding of the lectin, concanavalin A (Con A), to isolated hepatocytes was used to ascertain changes in the hepatic plasma membrane, especially in regard to glycoprotein composition, due to chronic ethanol feeding. Hepatocytes, isolated from rats fed ethanol for 5 to 7 weeks, had a decreased ability to bind Con A when compared to hepatocytes from either the pair-fed controls or ad libitum chow-fed rats. Since decreased Con A binding was more apparent at high Con A concentrations, reduced lectin binding likely reflected changes in the composition of surface membrane glycoproteins in the livers of the ethanol-fed rats. When ethanol (50 mM) was added to the incubation medium containing hepatocytes from ethanol-fed rats, pair-fed controls, or chow-fed rats, no effects on Con A binding were observed. These results indicate that chronic ethanol administration induces changes in the oligosaccharide chains of plasma membrane glycoproteins in the liver. Such alterations may play a role in the pathogenesis of alcoholic liver disease.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1525-1373</identifier><identifier>DOI: 10.3181/00379727-185-42508</identifier><identifier>PMID: 3575324</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>560300 - Chemicals Metabolism & Toxicology ; AGGLUTININS ; Alcoholism - immunology ; Alcoholism - pathology ; Alcoholism and acute alcohol poisoning ; ALCOHOLS ; ANIMAL CELLS ; ANIMALS ; ANTIBODIES ; BIOCHEMICAL REACTION KINETICS ; Biological and medical sciences ; BODY ; CARBOHYDRATES ; CELL CONSTITUENTS ; Cell Membrane - immunology ; Cell Membrane - ultrastructure ; CELL MEMBRANES ; CHRONIC INTAKE ; CONCANAVALIN ; DIGESTIVE SYSTEM ; DISEASES ; ETHANOL ; Ethanol - pharmacology ; GLANDS ; GLUCOPROTEINS ; HEMAGGLUTININS ; HYDROXY COMPOUNDS ; INTAKE ; KINETICS ; LECTINS ; LIVER ; Liver - drug effects ; Liver - pathology ; LIVER CELLS ; Male ; MAMMALS ; Medical sciences ; MEMBRANES ; METABOLIC DISEASES ; ORGANIC COMPOUNDS ; ORGANS ; PATHOGENESIS ; PROTEINS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RATS ; Rats, Inbred Strains ; REACTION KINETICS ; Receptors, Concanavalin A - metabolism ; RODENTS ; SACCHARIDES ; SOMATIC CELLS ; TOXICITY ; Toxicology ; VERTEBRATES</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1987-05, Vol.185 (1), p.1-5</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-9aaee87290354502d8b6dbc17b2f053efa3a4332af4801a9726121d82806e6333</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7532338$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3575324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6150104$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Metcalf, Jordan P.</creatorcontrib><creatorcontrib>Casey, Carol A.</creatorcontrib><creatorcontrib>Sorrell, Michael F.</creatorcontrib><creatorcontrib>Tuma, Dean J.</creatorcontrib><creatorcontrib>Veterans Administration Medical Center, Omaha, NE</creatorcontrib><title>Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Abstract
The effects of chronic ethanol administration on the hepatic surface membrane were examined. The binding of the lectin, concanavalin A (Con A), to isolated hepatocytes was used to ascertain changes in the hepatic plasma membrane, especially in regard to glycoprotein composition, due to chronic ethanol feeding. Hepatocytes, isolated from rats fed ethanol for 5 to 7 weeks, had a decreased ability to bind Con A when compared to hepatocytes from either the pair-fed controls or ad libitum chow-fed rats. Since decreased Con A binding was more apparent at high Con A concentrations, reduced lectin binding likely reflected changes in the composition of surface membrane glycoproteins in the livers of the ethanol-fed rats. When ethanol (50 mM) was added to the incubation medium containing hepatocytes from ethanol-fed rats, pair-fed controls, or chow-fed rats, no effects on Con A binding were observed. These results indicate that chronic ethanol administration induces changes in the oligosaccharide chains of plasma membrane glycoproteins in the liver. Such alterations may play a role in the pathogenesis of alcoholic liver disease.</description><subject>560300 - Chemicals Metabolism & Toxicology</subject><subject>AGGLUTININS</subject><subject>Alcoholism - immunology</subject><subject>Alcoholism - pathology</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>ALCOHOLS</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>ANTIBODIES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>Biological and medical sciences</subject><subject>BODY</subject><subject>CARBOHYDRATES</subject><subject>CELL CONSTITUENTS</subject><subject>Cell Membrane - immunology</subject><subject>Cell Membrane - ultrastructure</subject><subject>CELL MEMBRANES</subject><subject>CHRONIC INTAKE</subject><subject>CONCANAVALIN</subject><subject>DIGESTIVE SYSTEM</subject><subject>DISEASES</subject><subject>ETHANOL</subject><subject>Ethanol - pharmacology</subject><subject>GLANDS</subject><subject>GLUCOPROTEINS</subject><subject>HEMAGGLUTININS</subject><subject>HYDROXY COMPOUNDS</subject><subject>INTAKE</subject><subject>KINETICS</subject><subject>LECTINS</subject><subject>LIVER</subject><subject>Liver - drug effects</subject><subject>Liver - pathology</subject><subject>LIVER CELLS</subject><subject>Male</subject><subject>MAMMALS</subject><subject>Medical sciences</subject><subject>MEMBRANES</subject><subject>METABOLIC DISEASES</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PATHOGENESIS</subject><subject>PROTEINS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>REACTION KINETICS</subject><subject>Receptors, Concanavalin A - metabolism</subject><subject>RODENTS</subject><subject>SACCHARIDES</subject><subject>SOMATIC CELLS</subject><subject>TOXICITY</subject><subject>Toxicology</subject><subject>VERTEBRATES</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><issn>1525-1373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EKkvhCyAhWQhxC7U9cf4cl2WhSEUcgLM1cSZdV4m92EmlfnscdumR02g0v3mjeY-x11J8ANnIKyGgbmtVF7LRRam0aJ6wjdSgC6ja9inbrECxEs_Zi5TuhJC6VtUFuwBda1Dlhh13hxi8s3w_H9CHkW_7yXmX5oizC55vx5li4td0zL3lP5Y4oCX-jaYuoqfEMfH9vevJW-p598A_kY2EKTe74C16vMfRZR3-0fne-duX7NmAY6JX53rJfn3e_9xdFzffv3zdbW8KC6WYixaRqKlVK0CXWqi-6aq-s7Lu1CA00ICAJYDCoWyExPxjJZXsG9WIiioAuGRvT7ohzc4k62ayBxu8JzubSmohRZmh9yfoGMPvhdJsJpcsjWN-LSzJSC0hK4oMqhNoY0gp0mCO0U0YH4wUZs3C_MvC5CzM3yzy0puz-tJN1D-unM3P83fnOSaL45ANtS49YisEsMpcnbCEt2TuwhJ9Nu5_h_8AvJudfA</recordid><startdate>198705</startdate><enddate>198705</enddate><creator>Metcalf, Jordan P.</creator><creator>Casey, Carol A.</creator><creator>Sorrell, Michael F.</creator><creator>Tuma, Dean J.</creator><general>SAGE Publications</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>OTOTI</scope></search><sort><creationdate>198705</creationdate><title>Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding</title><author>Metcalf, Jordan P. ; Casey, Carol A. ; Sorrell, Michael F. ; Tuma, Dean J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-9aaee87290354502d8b6dbc17b2f053efa3a4332af4801a9726121d82806e6333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>560300 - Chemicals Metabolism & Toxicology</topic><topic>AGGLUTININS</topic><topic>Alcoholism - immunology</topic><topic>Alcoholism - pathology</topic><topic>Alcoholism and acute alcohol poisoning</topic><topic>ALCOHOLS</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>ANTIBODIES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>Biological and medical sciences</topic><topic>BODY</topic><topic>CARBOHYDRATES</topic><topic>CELL CONSTITUENTS</topic><topic>Cell Membrane - immunology</topic><topic>Cell Membrane - ultrastructure</topic><topic>CELL MEMBRANES</topic><topic>CHRONIC INTAKE</topic><topic>CONCANAVALIN</topic><topic>DIGESTIVE SYSTEM</topic><topic>DISEASES</topic><topic>ETHANOL</topic><topic>Ethanol - pharmacology</topic><topic>GLANDS</topic><topic>GLUCOPROTEINS</topic><topic>HEMAGGLUTININS</topic><topic>HYDROXY COMPOUNDS</topic><topic>INTAKE</topic><topic>KINETICS</topic><topic>LECTINS</topic><topic>LIVER</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>LIVER CELLS</topic><topic>Male</topic><topic>MAMMALS</topic><topic>Medical sciences</topic><topic>MEMBRANES</topic><topic>METABOLIC DISEASES</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PATHOGENESIS</topic><topic>PROTEINS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>REACTION KINETICS</topic><topic>Receptors, Concanavalin A - metabolism</topic><topic>RODENTS</topic><topic>SACCHARIDES</topic><topic>SOMATIC CELLS</topic><topic>TOXICITY</topic><topic>Toxicology</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Metcalf, Jordan P.</creatorcontrib><creatorcontrib>Casey, Carol A.</creatorcontrib><creatorcontrib>Sorrell, Michael F.</creatorcontrib><creatorcontrib>Tuma, Dean J.</creatorcontrib><creatorcontrib>Veterans Administration Medical Center, Omaha, NE</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>OSTI.GOV</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metcalf, Jordan P.</au><au>Casey, Carol A.</au><au>Sorrell, Michael F.</au><au>Tuma, Dean J.</au><aucorp>Veterans Administration Medical Center, Omaha, NE</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1987-05</date><risdate>1987</risdate><volume>185</volume><issue>1</issue><spage>1</spage><epage>5</epage><pages>1-5</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><eissn>1525-1373</eissn><coden>PSEBAA</coden><abstract>Abstract
The effects of chronic ethanol administration on the hepatic surface membrane were examined. The binding of the lectin, concanavalin A (Con A), to isolated hepatocytes was used to ascertain changes in the hepatic plasma membrane, especially in regard to glycoprotein composition, due to chronic ethanol feeding. Hepatocytes, isolated from rats fed ethanol for 5 to 7 weeks, had a decreased ability to bind Con A when compared to hepatocytes from either the pair-fed controls or ad libitum chow-fed rats. Since decreased Con A binding was more apparent at high Con A concentrations, reduced lectin binding likely reflected changes in the composition of surface membrane glycoproteins in the livers of the ethanol-fed rats. When ethanol (50 mM) was added to the incubation medium containing hepatocytes from ethanol-fed rats, pair-fed controls, or chow-fed rats, no effects on Con A binding were observed. These results indicate that chronic ethanol administration induces changes in the oligosaccharide chains of plasma membrane glycoproteins in the liver. Such alterations may play a role in the pathogenesis of alcoholic liver disease.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>3575324</pmid><doi>10.3181/00379727-185-42508</doi><tpages>5</tpages></addata></record> |
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| subjects | 560300 - Chemicals Metabolism & Toxicology AGGLUTININS Alcoholism - immunology Alcoholism - pathology Alcoholism and acute alcohol poisoning ALCOHOLS ANIMAL CELLS ANIMALS ANTIBODIES BIOCHEMICAL REACTION KINETICS Biological and medical sciences BODY CARBOHYDRATES CELL CONSTITUENTS Cell Membrane - immunology Cell Membrane - ultrastructure CELL MEMBRANES CHRONIC INTAKE CONCANAVALIN DIGESTIVE SYSTEM DISEASES ETHANOL Ethanol - pharmacology GLANDS GLUCOPROTEINS HEMAGGLUTININS HYDROXY COMPOUNDS INTAKE KINETICS LECTINS LIVER Liver - drug effects Liver - pathology LIVER CELLS Male MAMMALS Medical sciences MEMBRANES METABOLIC DISEASES ORGANIC COMPOUNDS ORGANS PATHOGENESIS PROTEINS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RATS Rats, Inbred Strains REACTION KINETICS Receptors, Concanavalin A - metabolism RODENTS SACCHARIDES SOMATIC CELLS TOXICITY Toxicology VERTEBRATES |
| title | Chronic Ethanol Administration Alters Hepatic Surface Membranes as Evidenced by Decreased Concanavalin A Binding |
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