Expression of biologically active, mature human granulocyte-macrophage colony stimulating factor with an E. coli secretory expression system

Human granulocyte-macrophage colony stimulating factor (HuGM-CSF) was expressed periplasmically in Escherichia coli with the secretory vector, pINIIIompA2. HuGM-CSF protein thus expressed was shown to be faithfully cleaved and biologically active. This protein, however, could not be released by osmo...

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Veröffentlicht in:	Current microbiology 1988-11, Vol.17 (6), p.321-332
Hauptverfasser:	GREENBERG, R, LUNDELL, D, NARULA, S. K, KASTELEIN, R, VAN KIMMENADE, A, ALROY, Y, BONITZ, S, CONDON, R, FOSSETTA, J, FROMMER, B, GEWAIN, K, KATZ, M, LEIBOWITZ, P. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Other active biomolecules Production of active biomolecules
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