Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton
Cotton (Gossypium hirsutum L.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynt...
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| Veröffentlicht in: | Plant physiology and biochemistry 2013-12, Vol.73, p.302-308 |
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| creator | Huang, Xinzheng Xiao, Yutao Köllner, Tobias G. Zhang, Wanna Wu, Junxiang Wu, Juan Guo, Yuyuan Zhang, Yongjun |
| description | Cotton (Gossypium hirsutum L.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, GhTPS1 and GhTPS2, were heterologously expressed and characterized. Recombinant GhTPS1 accepted farnesyl pyrophosphate as substrate and produced (E)-β-caryophyllene and α-humulene. GhTPS2 was characterized as a monoterpene synthase which formed α-pinene and β-pinene using geranyl pyrophosphate as substrate. Quantitative real-time PCR analysis revealed that GhTPS1 and GhTPS2 gene expression was elevated after methyl jasmonate (MeJA) treatment in cotton leaves. Moreover, feeding of the green plant bug Apolygus lucorum, a major cotton pest in northern China, resulted in increased GhTPS2 expression in young leaves, suggesting that GhTPS2 might be involved in plant defense in cotton.
[Display omitted]
•Two terpene synthases (GhTPS1 and GhTPS2) from cotton were characterized.•GhTPS1 accepted FPP as substrate and produced (E)-β-caryophyllene/α-humulene.•GhTPS2 formed α/β-pinene using GPP as substrate.•Their expressions in different organs as well as after herbivory were analyzed. |
| doi_str_mv | 10.1016/j.plaphy.2013.10.017 |
| format | Article |
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[Display omitted]
•Two terpene synthases (GhTPS1 and GhTPS2) from cotton were characterized.•GhTPS1 accepted FPP as substrate and produced (E)-β-caryophyllene/α-humulene.•GhTPS2 formed α/β-pinene using GPP as substrate.•Their expressions in different organs as well as after herbivory were analyzed.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2013.10.017</identifier><identifier>PMID: 24184450</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Acetates - metabolism ; Acetates - pharmacology ; Adaptation, Physiological ; Alkyl and Aryl Transferases - genetics ; Alkyl and Aryl Transferases - metabolism ; Animals ; Apolygus lucorum ; Biological and medical sciences ; Bridged Bicyclo Compounds - metabolism ; Carbon-Oxygen Lyases - genetics ; Carbon-Oxygen Lyases - metabolism ; China ; Cyclopentanes - metabolism ; Cyclopentanes - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Genes, Plant ; Gossypium - enzymology ; Gossypium - genetics ; Gossypium - metabolism ; Gossypium hirsutum ; Herbivory ; Insecta ; Methyl jasmonate ; Monoterpenes - metabolism ; Oxylipins - metabolism ; Oxylipins - pharmacology ; Plant defense ; Plant Diseases ; Plant Leaves - metabolism ; Plant physiology and development ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Polyisoprenyl Phosphates - metabolism ; Quantitative real-time PCR ; Sesquiterpenes - metabolism ; Terpenes - metabolism ; α-Pinene ; β-Caryophyllene</subject><ispartof>Plant physiology and biochemistry, 2013-12, Vol.73, p.302-308</ispartof><rights>2013 Elsevier Masson SAS</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-256719dd8832af144975bb67423300045143fd8aa832b3dfd1b0e0487acc6aa93</citedby><cites>FETCH-LOGICAL-c425t-256719dd8832af144975bb67423300045143fd8aa832b3dfd1b0e0487acc6aa93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plaphy.2013.10.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28024949$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24184450$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Xinzheng</creatorcontrib><creatorcontrib>Xiao, Yutao</creatorcontrib><creatorcontrib>Köllner, Tobias G.</creatorcontrib><creatorcontrib>Zhang, Wanna</creatorcontrib><creatorcontrib>Wu, Junxiang</creatorcontrib><creatorcontrib>Wu, Juan</creatorcontrib><creatorcontrib>Guo, Yuyuan</creatorcontrib><creatorcontrib>Zhang, Yongjun</creatorcontrib><title>Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Cotton (Gossypium hirsutum L.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, GhTPS1 and GhTPS2, were heterologously expressed and characterized. Recombinant GhTPS1 accepted farnesyl pyrophosphate as substrate and produced (E)-β-caryophyllene and α-humulene. GhTPS2 was characterized as a monoterpene synthase which formed α-pinene and β-pinene using geranyl pyrophosphate as substrate. Quantitative real-time PCR analysis revealed that GhTPS1 and GhTPS2 gene expression was elevated after methyl jasmonate (MeJA) treatment in cotton leaves. Moreover, feeding of the green plant bug Apolygus lucorum, a major cotton pest in northern China, resulted in increased GhTPS2 expression in young leaves, suggesting that GhTPS2 might be involved in plant defense in cotton.
[Display omitted]
•Two terpene synthases (GhTPS1 and GhTPS2) from cotton were characterized.•GhTPS1 accepted FPP as substrate and produced (E)-β-caryophyllene/α-humulene.•GhTPS2 formed α/β-pinene using GPP as substrate.•Their expressions in different organs as well as after herbivory were analyzed.</description><subject>Acetates - metabolism</subject><subject>Acetates - pharmacology</subject><subject>Adaptation, Physiological</subject><subject>Alkyl and Aryl Transferases - genetics</subject><subject>Alkyl and Aryl Transferases - metabolism</subject><subject>Animals</subject><subject>Apolygus lucorum</subject><subject>Biological and medical sciences</subject><subject>Bridged Bicyclo Compounds - metabolism</subject><subject>Carbon-Oxygen Lyases - genetics</subject><subject>Carbon-Oxygen Lyases - metabolism</subject><subject>China</subject><subject>Cyclopentanes - metabolism</subject><subject>Cyclopentanes - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>Genes, Plant</subject><subject>Gossypium - enzymology</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>Gossypium hirsutum</subject><subject>Herbivory</subject><subject>Insecta</subject><subject>Methyl jasmonate</subject><subject>Monoterpenes - metabolism</subject><subject>Oxylipins - metabolism</subject><subject>Oxylipins - pharmacology</subject><subject>Plant defense</subject><subject>Plant Diseases</subject><subject>Plant Leaves - metabolism</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Polyisoprenyl Phosphates - metabolism</subject><subject>Quantitative real-time PCR</subject><subject>Sesquiterpenes - metabolism</subject><subject>Terpenes - metabolism</subject><subject>α-Pinene</subject><subject>β-Caryophyllene</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGK1TAUhoMoznX0DUS6EcZF7yRN2qYbQYZRBwbc6DqkySk3l9ykJmnhzlsp-BrzTKbTq-JGVwkn33_Oyf8j9JLgLcGkudxvRyvH3XFbYUJzaYtJ-whtCG9pWTUdfow2uOOk7FjFz9CzGPcY44q19Ck6qxjhjNV4g37caHDJDEbJZLwrpNOF2skgVYJg7taiH4qL6zfl_fdSyXD0eai14KCIR5d2MsKD6v7bZQZG4_56GX1a-ktrj4Vxs7cz6HwplHcxmTQlM6_yHYTezD5AaZyeVKbyAuPSa_DhsO7xoEvJu-foySBthBen8xx9eX_9-epjefvpw83Vu9tSsapOZVU3Lem05pxWciCMdW3d903LKkqzGawmjA6aS5nfe6oHTXoMmPFWKtVI2dFzdLH2HYP_OkFM4mCiAmulAz9FQWqSW1Fe1_9HWcM47yhjGWUrqoKPMcAgxmAO2VlBsFiyFXuxZiuWbJdqzjbLXp0mTP0B9G_RrzAz8PoEyKikHYJ0ysQ_HM_xd2z51duVg2zdbCCIqAy47LkJoJLQ3vx7k59JEspj</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Huang, Xinzheng</creator><creator>Xiao, Yutao</creator><creator>Köllner, Tobias G.</creator><creator>Zhang, Wanna</creator><creator>Wu, Junxiang</creator><creator>Wu, Juan</creator><creator>Guo, Yuyuan</creator><creator>Zhang, Yongjun</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SS</scope></search><sort><creationdate>20131201</creationdate><title>Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton</title><author>Huang, Xinzheng ; Xiao, Yutao ; Köllner, Tobias G. ; Zhang, Wanna ; Wu, Junxiang ; Wu, Juan ; Guo, Yuyuan ; Zhang, Yongjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-256719dd8832af144975bb67423300045143fd8aa832b3dfd1b0e0487acc6aa93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acetates - metabolism</topic><topic>Acetates - pharmacology</topic><topic>Adaptation, Physiological</topic><topic>Alkyl and Aryl Transferases - genetics</topic><topic>Alkyl and Aryl Transferases - metabolism</topic><topic>Animals</topic><topic>Apolygus lucorum</topic><topic>Biological and medical sciences</topic><topic>Bridged Bicyclo Compounds - metabolism</topic><topic>Carbon-Oxygen Lyases - genetics</topic><topic>Carbon-Oxygen Lyases - metabolism</topic><topic>China</topic><topic>Cyclopentanes - metabolism</topic><topic>Cyclopentanes - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - drug effects</topic><topic>Genes, Plant</topic><topic>Gossypium - enzymology</topic><topic>Gossypium - genetics</topic><topic>Gossypium - metabolism</topic><topic>Gossypium hirsutum</topic><topic>Herbivory</topic><topic>Insecta</topic><topic>Methyl jasmonate</topic><topic>Monoterpenes - metabolism</topic><topic>Oxylipins - metabolism</topic><topic>Oxylipins - pharmacology</topic><topic>Plant defense</topic><topic>Plant Diseases</topic><topic>Plant Leaves - metabolism</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Polyisoprenyl Phosphates - metabolism</topic><topic>Quantitative real-time PCR</topic><topic>Sesquiterpenes - metabolism</topic><topic>Terpenes - metabolism</topic><topic>α-Pinene</topic><topic>β-Caryophyllene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Xinzheng</creatorcontrib><creatorcontrib>Xiao, Yutao</creatorcontrib><creatorcontrib>Köllner, Tobias G.</creatorcontrib><creatorcontrib>Zhang, Wanna</creatorcontrib><creatorcontrib>Wu, Junxiang</creatorcontrib><creatorcontrib>Wu, Juan</creatorcontrib><creatorcontrib>Guo, Yuyuan</creatorcontrib><creatorcontrib>Zhang, Yongjun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Xinzheng</au><au>Xiao, Yutao</au><au>Köllner, Tobias G.</au><au>Zhang, Wanna</au><au>Wu, Junxiang</au><au>Wu, Juan</au><au>Guo, Yuyuan</au><au>Zhang, Yongjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>73</volume><spage>302</spage><epage>308</epage><pages>302-308</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>Cotton (Gossypium hirsutum L.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, GhTPS1 and GhTPS2, were heterologously expressed and characterized. Recombinant GhTPS1 accepted farnesyl pyrophosphate as substrate and produced (E)-β-caryophyllene and α-humulene. GhTPS2 was characterized as a monoterpene synthase which formed α-pinene and β-pinene using geranyl pyrophosphate as substrate. Quantitative real-time PCR analysis revealed that GhTPS1 and GhTPS2 gene expression was elevated after methyl jasmonate (MeJA) treatment in cotton leaves. Moreover, feeding of the green plant bug Apolygus lucorum, a major cotton pest in northern China, resulted in increased GhTPS2 expression in young leaves, suggesting that GhTPS2 might be involved in plant defense in cotton.
[Display omitted]
•Two terpene synthases (GhTPS1 and GhTPS2) from cotton were characterized.•GhTPS1 accepted FPP as substrate and produced (E)-β-caryophyllene/α-humulene.•GhTPS2 formed α/β-pinene using GPP as substrate.•Their expressions in different organs as well as after herbivory were analyzed.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>24184450</pmid><doi>10.1016/j.plaphy.2013.10.017</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0981-9428 |
| ispartof | Plant physiology and biochemistry, 2013-12, Vol.73, p.302-308 |
| issn | 0981-9428 1873-2690 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acetates - metabolism Acetates - pharmacology Adaptation, Physiological Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism Animals Apolygus lucorum Biological and medical sciences Bridged Bicyclo Compounds - metabolism Carbon-Oxygen Lyases - genetics Carbon-Oxygen Lyases - metabolism China Cyclopentanes - metabolism Cyclopentanes - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Genes, Plant Gossypium - enzymology Gossypium - genetics Gossypium - metabolism Gossypium hirsutum Herbivory Insecta Methyl jasmonate Monoterpenes - metabolism Oxylipins - metabolism Oxylipins - pharmacology Plant defense Plant Diseases Plant Leaves - metabolism Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Polyisoprenyl Phosphates - metabolism Quantitative real-time PCR Sesquiterpenes - metabolism Terpenes - metabolism α-Pinene β-Caryophyllene |
| title | Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton |
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