Comparative transcriptome pairwise analysis of spontaneously transformed multipotent stromal cells from human adipose tissue
Potential markers and the mechanism of the spontaneous transformation of multipotent stromal cells (MSC) with perivascular immunophenotype have been determined. A transcriptome comparative study was performed involving six paired specimens of normal and spontaneously transformed MSC with perivascula...
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| Veröffentlicht in: | Russian journal of genetics 2014, Vol.50 (1), p.96-104 |
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| creator | Omelchenko, D. O Rzhaninova, A. A Goldshtein, D. V |
| description | Potential markers and the mechanism of the spontaneous transformation of multipotent stromal cells (MSC) with perivascular immunophenotype have been determined. A transcriptome comparative study was performed involving six paired specimens of normal and spontaneously transformed MSC with perivascular immunophenotype, obtained in the first passages following the isolation of adipose tissue of six healthy donors. According to the results obtained using the microarray Illumina HT-12 v4 with the Significance Analysis of Microarrays software, differentially expressed transcripts were revealed and a statistical analysis using Gene Ontology and Molecular Signatures Databases was conducted. The association of the spontaneous transformation of isolated MSC with perivascular immunophenotype with previously identified oncogenic cell transformation pathways (E2F, ATR/ATM, RAS, and RHOA) is suggested and further aims for more detailed study are set. Potential transformation markers, including largely unknown genes and those previously not associated with cancer genes (HSPB6, PLAC9, FEZ1, DTWD1, APH1A, and ATP5L), are described. |
| doi_str_mv | 10.1134/S1022795414010098 |
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O ; Rzhaninova, A. A ; Goldshtein, D. V</creator><creatorcontrib>Omelchenko, D. O ; Rzhaninova, A. A ; Goldshtein, D. V</creatorcontrib><description>Potential markers and the mechanism of the spontaneous transformation of multipotent stromal cells (MSC) with perivascular immunophenotype have been determined. A transcriptome comparative study was performed involving six paired specimens of normal and spontaneously transformed MSC with perivascular immunophenotype, obtained in the first passages following the isolation of adipose tissue of six healthy donors. According to the results obtained using the microarray Illumina HT-12 v4 with the Significance Analysis of Microarrays software, differentially expressed transcripts were revealed and a statistical analysis using Gene Ontology and Molecular Signatures Databases was conducted. The association of the spontaneous transformation of isolated MSC with perivascular immunophenotype with previously identified oncogenic cell transformation pathways (E2F, ATR/ATM, RAS, and RHOA) is suggested and further aims for more detailed study are set. Potential transformation markers, including largely unknown genes and those previously not associated with cancer genes (HSPB6, PLAC9, FEZ1, DTWD1, APH1A, and ATP5L), are described.</description><identifier>ISSN: 1022-7954</identifier><identifier>EISSN: 1608-3369</identifier><identifier>DOI: 10.1134/S1022795414010098</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>adipose tissue ; Animal Genetics and Genomics ; Biomedical and Life Sciences ; Biomedicine ; computer software ; gene expression regulation ; genes ; Human Genetics ; humans ; microarray technology ; Microbial Genetics and Genomics ; statistical analysis ; stromal cells ; transcriptome</subject><ispartof>Russian journal of genetics, 2014, Vol.50 (1), p.96-104</ispartof><rights>Pleiades Publishing, Inc. 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-b49445394afd12c901819d17f93b6c1536b8fdeb0996064200a58590b1dcff583</citedby><cites>FETCH-LOGICAL-c345t-b49445394afd12c901819d17f93b6c1536b8fdeb0996064200a58590b1dcff583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1022795414010098$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1022795414010098$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,4010,27904,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Omelchenko, D. 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According to the results obtained using the microarray Illumina HT-12 v4 with the Significance Analysis of Microarrays software, differentially expressed transcripts were revealed and a statistical analysis using Gene Ontology and Molecular Signatures Databases was conducted. The association of the spontaneous transformation of isolated MSC with perivascular immunophenotype with previously identified oncogenic cell transformation pathways (E2F, ATR/ATM, RAS, and RHOA) is suggested and further aims for more detailed study are set. Potential transformation markers, including largely unknown genes and those previously not associated with cancer genes (HSPB6, PLAC9, FEZ1, DTWD1, APH1A, and ATP5L), are described.</description><subject>adipose tissue</subject><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>computer software</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>Human Genetics</subject><subject>humans</subject><subject>microarray technology</subject><subject>Microbial Genetics and Genomics</subject><subject>statistical analysis</subject><subject>stromal cells</subject><subject>transcriptome</subject><issn>1022-7954</issn><issn>1608-3369</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kc1OxSAQhYnRRL36AK5k6abKAK1laW78S0xcqGtCW1BMWypDNTfx4eWm7kxcATnnO5k5EHIC7BxAyIsnYJxfqlKCZMCYqnfIAVSsLoSo1G6-Z7nY6vvkEPGdbU2VOCDf6zBMJprkPy1N0YzYRj-lMFg6GR-_PFpqRtNv0CMNjuIUxmRGG2bsNwvgQhxsR4e5T34KyY6JYophMD1tbd8jdflB3-bBjNR02ZIjk0ec7RHZc6ZHe_x7rsjLzfXz-q54eLy9X189FK2QZSoaqaQshZLGdcBbxaAG1cGlU6KpWihF1dSusw1TqmKV5IyZsi4Va6BrnStrsSJnS-4Uw8dsMenB43a2ZRENJXDB6zo3uSKwWNsYEKN1eop-MHGjgelt0_pP05nhC4PZO77aqN_DHHNp-C90ukDOBG1eo0f98sRZ1vPfCJ6pH5FujCA</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Omelchenko, D. 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According to the results obtained using the microarray Illumina HT-12 v4 with the Significance Analysis of Microarrays software, differentially expressed transcripts were revealed and a statistical analysis using Gene Ontology and Molecular Signatures Databases was conducted. The association of the spontaneous transformation of isolated MSC with perivascular immunophenotype with previously identified oncogenic cell transformation pathways (E2F, ATR/ATM, RAS, and RHOA) is suggested and further aims for more detailed study are set. Potential transformation markers, including largely unknown genes and those previously not associated with cancer genes (HSPB6, PLAC9, FEZ1, DTWD1, APH1A, and ATP5L), are described.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><doi>10.1134/S1022795414010098</doi><tpages>9</tpages></addata></record> |
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| subjects | adipose tissue Animal Genetics and Genomics Biomedical and Life Sciences Biomedicine computer software gene expression regulation genes Human Genetics humans microarray technology Microbial Genetics and Genomics statistical analysis stromal cells transcriptome |
| title | Comparative transcriptome pairwise analysis of spontaneously transformed multipotent stromal cells from human adipose tissue |
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