Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer
•Stearyl-[D]-K6L9 is a novel type of antimicrobial peptide-based gene vector.•Stearyl-[D]-K6L9 delivers plasmids into cells by endocytosis.•Stearyl-[D]-K6L9 displays high endosome-lytic activity.•Stearyl-[D]-K6L9 displays high transfection efficiency. Stearyl-cell penetrating peptides (CPPs) have be...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2013-08, Vol.46, p.33-39 |
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| container_title | Peptides (New York, N.Y. : 1980) |
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| creator | Zhang, Wei Song, Jingjing Liang, Ranran Zheng, Xin Chen, Jianbo Li, Guolin Zhang, Bangzhi Wang, Kairong Yan, Xiang Wang, Rui |
| description | •Stearyl-[D]-K6L9 is a novel type of antimicrobial peptide-based gene vector.•Stearyl-[D]-K6L9 delivers plasmids into cells by endocytosis.•Stearyl-[D]-K6L9 displays high endosome-lytic activity.•Stearyl-[D]-K6L9 displays high transfection efficiency.
Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide [D]-K6L9. In this study, stearyl-[D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-[D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with [D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors. |
| doi_str_mv | 10.1016/j.peptides.2013.05.011 |
| format | Article |
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Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide [D]-K6L9. In this study, stearyl-[D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-[D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with [D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/j.peptides.2013.05.011</identifier><identifier>PMID: 23727033</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antimicrobial Cationic Peptides - genetics ; Antimicrobial Cationic Peptides - metabolism ; Antimicrobial peptide ; antimicrobial peptides ; Cell Line ; Cell penetrating peptide ; Cell-Penetrating Peptides - genetics ; Cell-Penetrating Peptides - metabolism ; Cercopithecus aethiops ; CHO Cells ; COS Cells ; Cricetulus ; Endocytosis ; Endocytosis - drug effects ; Endocytosis - physiology ; Gene Transfer Techniques ; genes ; Genetic Therapy - methods ; Genetic Vectors - genetics ; Green Fluorescent Proteins - genetics ; Lipids ; Nonviral gene vector ; peptides ; plasmids ; Stearylation ; transfection</subject><ispartof>Peptides (New York, N.Y. : 1980), 2013-08, Vol.46, p.33-39</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-d1a4d44349dea0b5ff74dfe1bd6f4b7a0d59bf4cbe5aadf1745b9e11756365793</citedby><cites>FETCH-LOGICAL-c425t-d1a4d44349dea0b5ff74dfe1bd6f4b7a0d59bf4cbe5aadf1745b9e11756365793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0196978113002027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23727033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Song, Jingjing</creatorcontrib><creatorcontrib>Liang, Ranran</creatorcontrib><creatorcontrib>Zheng, Xin</creatorcontrib><creatorcontrib>Chen, Jianbo</creatorcontrib><creatorcontrib>Li, Guolin</creatorcontrib><creatorcontrib>Zhang, Bangzhi</creatorcontrib><creatorcontrib>Wang, Kairong</creatorcontrib><creatorcontrib>Yan, Xiang</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><title>Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>•Stearyl-[D]-K6L9 is a novel type of antimicrobial peptide-based gene vector.•Stearyl-[D]-K6L9 delivers plasmids into cells by endocytosis.•Stearyl-[D]-K6L9 displays high endosome-lytic activity.•Stearyl-[D]-K6L9 displays high transfection efficiency.
Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide [D]-K6L9. In this study, stearyl-[D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-[D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with [D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors.</description><subject>Animals</subject><subject>Antimicrobial Cationic Peptides - genetics</subject><subject>Antimicrobial Cationic Peptides - metabolism</subject><subject>Antimicrobial peptide</subject><subject>antimicrobial peptides</subject><subject>Cell Line</subject><subject>Cell penetrating peptide</subject><subject>Cell-Penetrating Peptides - genetics</subject><subject>Cell-Penetrating Peptides - metabolism</subject><subject>Cercopithecus aethiops</subject><subject>CHO Cells</subject><subject>COS Cells</subject><subject>Cricetulus</subject><subject>Endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Endocytosis - physiology</subject><subject>Gene Transfer Techniques</subject><subject>genes</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors - genetics</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Lipids</subject><subject>Nonviral gene vector</subject><subject>peptides</subject><subject>plasmids</subject><subject>Stearylation</subject><subject>transfection</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERZeWv1B85JLgie04voFavsRKPbQ9IWQ58XjxajcJthfUf4-j3XLtyQc_M_PqeQm5AlYDg_b9tp5xzsFhqhsGvGayZgAvyAo6xSsJrX5JVgx0W2nVwTl5ndKWMSaE7l6R84arRjHOVwTvMtr4uLMZHbVjDvswxKkPdkdPB-iPm5_V93at6d-Qf9EBd8vXiDnaHMYNneM0Y8yP1E-RovdhCDhmuikILcyYPMZLcubtLuGb03tBHj5_ur_-Wq1vv3y7_riuBtHIXDmwwgnBhXZoWS-9V8J5hN61XvTKMid178XQo7TWeVBC9hoBlGx5K5XmF-TdcW8J9fuAKZt9SEtiO-J0SAYkNLwRDMTzqIAORNc1rKDtES1mUorozRzDvlgzwMzShtmapzbM0oZh0pQ2yuDV6cah36P7P_akvwBvj4C3k7GbGJJ5uCsb2lIV6zioQnw4Eli0_QkYTVr8DuhCxCEbN4XnUvwDzwapVQ</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Zhang, Wei</creator><creator>Song, Jingjing</creator><creator>Liang, Ranran</creator><creator>Zheng, Xin</creator><creator>Chen, Jianbo</creator><creator>Li, Guolin</creator><creator>Zhang, Bangzhi</creator><creator>Wang, Kairong</creator><creator>Yan, Xiang</creator><creator>Wang, Rui</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130801</creationdate><title>Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer</title><author>Zhang, Wei ; Song, Jingjing ; Liang, Ranran ; Zheng, Xin ; Chen, Jianbo ; Li, Guolin ; Zhang, Bangzhi ; Wang, Kairong ; Yan, Xiang ; Wang, Rui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-d1a4d44349dea0b5ff74dfe1bd6f4b7a0d59bf4cbe5aadf1745b9e11756365793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antimicrobial Cationic Peptides - genetics</topic><topic>Antimicrobial Cationic Peptides - metabolism</topic><topic>Antimicrobial peptide</topic><topic>antimicrobial peptides</topic><topic>Cell Line</topic><topic>Cell penetrating peptide</topic><topic>Cell-Penetrating Peptides - genetics</topic><topic>Cell-Penetrating Peptides - metabolism</topic><topic>Cercopithecus aethiops</topic><topic>CHO Cells</topic><topic>COS Cells</topic><topic>Cricetulus</topic><topic>Endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Endocytosis - physiology</topic><topic>Gene Transfer Techniques</topic><topic>genes</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors - genetics</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Lipids</topic><topic>Nonviral gene vector</topic><topic>peptides</topic><topic>plasmids</topic><topic>Stearylation</topic><topic>transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Song, Jingjing</creatorcontrib><creatorcontrib>Liang, Ranran</creatorcontrib><creatorcontrib>Zheng, Xin</creatorcontrib><creatorcontrib>Chen, Jianbo</creatorcontrib><creatorcontrib>Li, Guolin</creatorcontrib><creatorcontrib>Zhang, Bangzhi</creatorcontrib><creatorcontrib>Wang, Kairong</creatorcontrib><creatorcontrib>Yan, Xiang</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Wei</au><au>Song, Jingjing</au><au>Liang, Ranran</au><au>Zheng, Xin</au><au>Chen, Jianbo</au><au>Li, Guolin</au><au>Zhang, Bangzhi</au><au>Wang, Kairong</au><au>Yan, Xiang</au><au>Wang, Rui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>46</volume><spage>33</spage><epage>39</epage><pages>33-39</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><abstract>•Stearyl-[D]-K6L9 is a novel type of antimicrobial peptide-based gene vector.•Stearyl-[D]-K6L9 delivers plasmids into cells by endocytosis.•Stearyl-[D]-K6L9 displays high endosome-lytic activity.•Stearyl-[D]-K6L9 displays high transfection efficiency.
Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide [D]-K6L9. In this study, stearyl-[D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-[D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with [D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23727033</pmid><doi>10.1016/j.peptides.2013.05.011</doi><tpages>7</tpages></addata></record> |
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| ispartof | Peptides (New York, N.Y. : 1980), 2013-08, Vol.46, p.33-39 |
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| subjects | Animals Antimicrobial Cationic Peptides - genetics Antimicrobial Cationic Peptides - metabolism Antimicrobial peptide antimicrobial peptides Cell Line Cell penetrating peptide Cell-Penetrating Peptides - genetics Cell-Penetrating Peptides - metabolism Cercopithecus aethiops CHO Cells COS Cells Cricetulus Endocytosis Endocytosis - drug effects Endocytosis - physiology Gene Transfer Techniques genes Genetic Therapy - methods Genetic Vectors - genetics Green Fluorescent Proteins - genetics Lipids Nonviral gene vector peptides plasmids Stearylation transfection |
| title | Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer |
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